Role of ROS in Aβ42 mediated cell surface P-selectin expression and actin polymerization

Tsoy, Andrey; Askarova, Sholpan; Shalakhmetova, Tamara; Umbayev, Bauyrzhan; Adambekov, Shalkar; Zhumadilov, Z.

doi:10.1186/1750-1326-8-s1-p44

Cited by 1 publication

(3 citation statements)

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“…In cells that were incubated only with plant extract, the P-selectin content on the surface did not differ from the control level (see Figure 6b). We have previously reported that phosphorylation of ERK 1/2 in astrocytes and mobilization of P-selectin in CECs could be induced by ROS [25,30]. As a positive control, in the present study, we have exposed the cells to H 2 O 2 (see Figures 5 and 6).…”

Section: Pretreatment With L Gmelinii Extract Suppressed Induced By Tnf-α Pro-inflammatory Responses In Astrocytes and Cecsmentioning

confidence: 80%

“…Transmigration of immunocompetent blood cells is one of the earliest stages of the development of inflammation in the brain parenchyma [40]. It is regulated by cell adhesion receptors expressed on the surface of CECs, such as selectins [30,40]. It is known that ROS and TNF-α lead to the activation of CECs, followed by the mobilization of P-selectin on the luminal surface of the endothelium [30,41].…”

Section: Discussionmentioning

confidence: 99%

“…It is regulated by cell adhesion receptors expressed on the surface of CECs, such as selectins [30,40]. It is known that ROS and TNF-α lead to the activation of CECs, followed by the mobilization of P-selectin on the luminal surface of the endothelium [30,41]. In connection with this, one of the tasks of this study was to investigate the effect of polyphenols extract from L. gmelinii on the TNF-α-induced mobilization of P-selectin.…”

Section: Discussionmentioning

confidence: 99%

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Plant Extract of Limonium gmelinii Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion

et al. 2021

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There are numerous publications demonstrating that plant polyphenols can reduce oxidative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H2O2, and TNF-α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P-selectin expression, and activity of ERK1/2 were evaluated by quantitative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF-α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF-α induced oxidative stress and triggered the accumulation of P-selectin on the surface of the cells. In turn, pre-treatment of the cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro-inflammatory responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor activity in rats with MCAO.

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Section: Pretreatment With L Gmelinii Extract Suppressed Induced By Tnf-α Pro-inflammatory Responses In Astrocytes and Cecsmentioning

confidence: 80%