Role of Reactive Oxygen Species during Low-Intensity Pulsed Ultrasound Application in MC-3 T3 E1 Pre-osteoblast Cell Culture

Kaur, Harmanpreet; Siraki, Arno G.; Uludağ, Hasan; Dederich, Douglas N.; Flood, Patrick M.; El‐Bialy, Tarek

doi:10.1016/j.ultrasmedbio.2017.07.002

Cited by 8 publications

(6 citation statements)

References 49 publications

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“…These receptors further initiate the cascade of cellular and molecular events in the cell known as mechanotransduction. Several cellular signaling pathways, like focal adhesion kinase (FAK) [36], mitogen-activated protein kinase (MAPK) [37], and Rho pathways [38], have shown to be activated in the in-vitro studies with LIPUS application. Through these mechanisms, LIPUS has shown to enhance bone formation and osteoblast differentiation in fracture healing cases.…”

Section: Discussionmentioning

confidence: 99%

Shortening of Overall Orthodontic Treatment Duration with Low-Intensity Pulsed Ultrasound (LIPUS)

Kaur

El‐Bialy

2020

JCM

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The aim of this retrospective clinical study was to determine if there is a reduction in the overall treatment duration in orthodontic patients using low-intensity pulsed ultrasound (LIPUS) and Invisalign SmartTrack® clear aligners. Data were collected from the first thirty-four patients (9 males, 25 females; average age 41.37 ± 15.02) who finished their orthodontic treatment using an intraoral LIPUS device and Invisalign clear aligners in a private clinic. The LIPUS parameters used by patients at home for 20 min/day were: ultrasonic frequency 1.5 MHz, pulse duration 200µs, pulse repetition rate 1 kHz, and spatial average-temporal average intensity 30mW/cm2. A control group (11 males, 23 females; average age 31.36 ± 14.41) matching for the same malocclusions was randomly selected from finished treatment cases of the same clinician. The date of first Invisalign attachment placement and first use of LIPUS application was recorded as T0, and the date of retainer delivery was recorded as T1. The treatment duration (T1–T0) and treatment reduction percentage with LIPUS device were collected and analyzed using two-sample t-test in Microsoft Excel. Treatment duration was significantly reduced in the LIPUS group (541.44 ± 192.23 days) compared to control group (1061.05 ± 455.64 days) (p < 0.05). The LIPUS group showed on average 49% reduction in the overall treatment time as compared to the control group. The average compliance of the patients using LIPUS was 66.02%. Patients who used LIPUS showed a clinically significant reduction in the overall orthodontic treatment duration compared to the control group who used Invisalign clear aligners only.

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Section: Discussionmentioning

confidence: 99%

Shortening of Overall Orthodontic Treatment Duration with Low-Intensity Pulsed Ultrasound (LIPUS)

Kaur

El‐Bialy

2020

JCM

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“…Osteocytes express NO transporters and a few minutes after a mechanical stimulus, release NO which suppresses bone resorption ( Chambers et al, 1999 ). Increased ROS levels and osteogenic gene expression in MC3T3-E1 osteoblasts, through MAPK activation mediated by ROS, were described after stimulation by low-intensity pulsed ultrasounds; inhibition of ROS attenuated osteogenic gene expression, including Runx2, osteocalcin, and osteopontin ( Kaur et al, 2017 ). It is also recognized that osteocytes transduce mechanical load signals to activate Nox2, producing ROS signals; this response is accompanied by a rapid ROS-mediated decrease in sclerostin levels by lysosomes, allowing activation of the Wnt/β-catenin signaling pathway, and bone formation ( Schröder, 2015 ; Gould et al, 2021 ).…”

Section: Oxidative Stress and Bone Mechanobiologymentioning

confidence: 99%

In Sickness and in Health: The Oxygen Reactive Species and the Bone

Reis

Ramos

2021

Front. Bioeng. Biotechnol.

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Oxidative stress plays a central role in physiological and pathological bone conditions. Its role in signalment and control of bone cell population differentiation, activity, and fate is increasingly recognized. The possibilities of its use and manipulation with therapeutic goals are virtually unending. However, how redox balance interplays with the response to mechanical stimuli is yet to be fully understood. The present work summarizes current knowledge on these aspects, in an integrative and broad introductory perspective.

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“…29,40 In addition, LIPUS stimulation could stimulate MC3T3-E1 preosteoblasts to produce reactive oxygen species to activate the mitogen-activated protein kinase pathway, resulting in a significant increase in cell viability. 19 Furthermore, LIPUS could accelerate the uptake of calcium and phosphate by osteoblast precursor cells to promote their mineralization. Tabuchi et al 33 confirmed that many genes in MC3T3-E1 preosteoblasts respond to LIPUS stimulation, such as Dmp1 , Fbn1 , Igf2 , Lum , Mmp13 , and Thbs1 , and are related to osteoblast differentiation and bone morphology.…”

Section: Discussionmentioning

confidence: 99%

Effect of Low-Intensity Pulsed Ultrasound on the Graft-Bone Healing of Artificial Ligaments: An In Vitro and In Vivo Study

Liu

Sun

et al. 2022

Am J Sports Med

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Background: As many researchers have focused on promoting the graft-bone healing of artificial ligaments, even with numerous chemical coatings, identifying a biosafe, effective, and immediately usable method is still important clinically. Purpose: (1) To determine whether a low-intensity pulsed ultrasound system (LIPUS) promotes in vitro cell viability and osteogenic differentiation and (2) to assess the applicability and effectiveness of LIPUS in promoting the graft-bone healing of artificial ligaments in vivo. Study Design: Controlled laboratory study. Methods: Polyethylene terephthalate (PET) sheets and grafts were randomly assigned to control and LIPUS groups. MC3T3-E1 preosteoblasts were cultured on PET sheets. Cell viability and morphology were evaluated using a live/dead viability assay and scanning electron microscopy. Alkaline phosphatase activity, calcium nodule formation, and Western blot were evaluated for osteogenic differentiation. For in vivo experiments, the effect of LIPUS was evaluated via an extra-articular graft-bone healing model in 48 rabbits: the osteointegration and new bone formation were tested by micro–computed tomography and histological staining, and the graft-bone bonding was tested by biomechanical testing. Results: Cell viability was significantly higher in the LIPUS group as compared with control (living and dead compared between control and LIPUS groups, P = .0489 vs P = .0489). Better adherence of cells and greater development of extracellular matrix were observed in the LIPUS group. Furthermore, LIPUS promoted alkaline phosphatase activity, calcium nodule formation, and the protein expression of collagen 1 ( P = .0002) and osteocalcin ( P = .0006) in vitro. Micro–computed tomography revealed higher surrounding bone mass at 4 weeks and newly formed bone mass at 8 weeks in the LIPUS group ( P = .0014 and P = .0018). Histological analysis showed a narrower interface and direct graft-bone contact in the LIPUS group; the surrounding bone area at 4 weeks and the mass of newly formed bone at 4 and 8 weeks in the LIPUS group were also significantly higher as compared with control (surrounding bone, P < .0001; newly formed bone, P = .0016 at 4 weeks and P = .005 at 8 weeks). The ultimate failure load in the LIPUS group was significantly higher than in the control group ( P < .0001 at 4 weeks; P = .0008 at 8 weeks). Conclusion: LIPUS promoted the viability and osteogenic differentiation of MC3T3-E1 preosteoblasts in vitro and enhanced the graft-bone healing of PET artificial ligament in vivo. Clinical Relevance: LIPUS is an effective physical stimulation to enhance graft-bone healing after artificial ligament implantation.

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Role of Reactive Oxygen Species during Low-Intensity Pulsed Ultrasound Application in MC-3 T3 E1 Pre-osteoblast Cell Culture

Cited by 8 publications

References 49 publications

Shortening of Overall Orthodontic Treatment Duration with Low-Intensity Pulsed Ultrasound (LIPUS)

Shortening of Overall Orthodontic Treatment Duration with Low-Intensity Pulsed Ultrasound (LIPUS)

In Sickness and in Health: The Oxygen Reactive Species and the Bone

Effect of Low-Intensity Pulsed Ultrasound on the Graft-Bone Healing of Artificial Ligaments: An In Vitro and In Vivo Study

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