In Sickness and in Health: The Oxygen Reactive Species and the Bone

Reis, Joana; Ramos, António

doi:10.3389/fbioe.2021.745911

Cited by 10 publications

(10 citation statements)

References 135 publications

(176 reference statements)

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“…Overall, our data indicate that Dpp3 preserves bone health, which sounds reasonable based on evidence of reduced Nrf2 and HO-1 expression and increased lipid peroxidation in the bone tissue lacking Dpp3 and on the well-known detrimental effect of sustained inflammation and oxidative stress on bone homeostasis [17].…”

Section: Discussionsupporting

confidence: 78%

Dipeptidyl Peptidase 3 Activity as a Promising Biomarker of Bone Fragility in Postmenopausal Women

et al. 2022

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The dipeptidyl peptidase 3 (Dpp3) is a ubiquitous zinc-dependent aminopeptidase, participating in the activation or degradation of signaling peptides and in the Keap1–Nrf2 antioxidant pathway. The absence of Dpp3 in the Dpp3 knockout mouse model causes increased osteoclast activity, altered osteogenic function, sustained oxidative stress in the bone tissue, and bone loss. We aimed to assess the association of Dpp3 activity with bone fragility in postmenopausal osteoporosis and the impact of denosumab on enzymatic activity. We conducted a two-phase study including 69 postmenopausal women with severe osteoporosis and 36 postmenopausal women without osteometabolic conditions, as controls (cross-sectional phase). Subjects with severe osteoporosis were assessed at baseline and 14 days after the first denosumab administration (prospective phase). The results showed significant reduction in serum Dpp3 activity (expressed as nmoles of formed product/mg proteins/min) in patients vs. controls (0.791 ± 0.232 vs. 1.195 ± 0.338; p < 0.001), and significant association with bone mass at the femoral neck (r = 0.28, p = 0.02) in patients prior to treatment. We found a negative correlation between C-terminal telopeptide (CTX) or N-terminal pro-peptide of type 1 procollagen (P1NP) levels and Dpp3 activity (respectively, r = −0.29, p = 0.012; and r = −0.2572, p = 0.033). Dpp3 activity did not change after denosumab injection. Our findings support a critical role played by Dpp3 in bone homeostasis as a potential bone protective factor. Additional clinical studies in larger cohorts might explore the implementation of Dpp3 assessment as a biomarker of bone health status.

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Section: Discussionsupporting

confidence: 78%

Dipeptidyl Peptidase 3 Activity as a Promising Biomarker of Bone Fragility in Postmenopausal Women

et al. 2022

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“…Since the progression of GIOP is inextricably linked to oxidative stress, we managed to evaluate oxidative stress markers 16 (Fig. 4 b).…”

Section: Resultsmentioning

confidence: 99%

“…Administration of AcE and AcCF suppresses oxidative stress in GIOP mice. Since the progression of GIOP is inextricably linked to oxidative stress, we managed to evaluate oxidative stress markers 16 (Fig. 4b).…”

Section: Determination Of Calcium Content In Ace Andmentioning

confidence: 99%

“…Because inflammation and oxidative stress are intricately connected to osteoporosis progression, Allolobophora calignosa coelomic fluid (AcCF) and Allolobophora calignosa extract (AcE) are hypothesized to have antiosteoporosis capabilities 16 . So, this study aimed to investigate the possible anti-osteoporotic effect of AcE and AcCF against GIOP in male mice.…”

Section: Introductionmentioning

confidence: 99%

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Allolobophora caliginosa coelomic fluid and extract alleviate glucocorticoid-induced osteoporosis in mice by suppressing oxidative stress and regulating osteoblastic/osteoclastic-related markers

Abdelfattah

Mohamed

Ibrahim

et al. 2023

Sci Rep

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Allolobophora calignosa (Ac) is a folk medicine for millennia, as it possesses many biological activities. This study aimed to investigate the chemo-preventive activity of A.calignosa coelomic fluid (AcCF) and A.calignosa extract (AcE) on glucocorticoid-induced osteoporosis (GIOP) in mice. Characterization and in vitro biological activity of AcE and AcCF has been assessed. Male CD-1 mice were subcutaneously received dexamethasone (DEX) (1 mg/kg, 5 times/week) and concurrently intraperitoneally treated with either AcCF (20 mg/kg) or AcE (45 mg/kg) every other day for 28 days. Serum and bone homogenates were subjected for qPCR and biochemical analysis. AcE and AcCF treatment significantly increased bone mineral density (BMD), bone mineral content (BMC), calcium (Ca), phosphorus (P), and calcitonin levels, whereas activity of serum alkaline phosphatase (ALP), bone alkaline phosphatase (BALP), serum acidic phosphatase (ACP), bone acidic phosphatase (BACP) and parathyroid hormone (PTH) levels were significantly reduced compare with untreated GIOP mice. Treatment with AcE and AcCF modulates oxidative stress and downregulated Rank and Mmp9 expression, as well as increased glycosaminoglycan content in the organic bone matrix, resulting in osteoclastogenesis inhibition. Overall, AcCF and AcE show a chemo-preventive activity against GIOP by inhibiting oxidative stress and regulating expression and/or activity of osteoblast/osteoclast-related markers.

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“…Oxidative stress has been linked to many bone-related diseases in humans and mammals ( Reis and Ramos, 2021 ). In broilers, at least 30% of birds showed poor locomotion during the fast growth period ( Wideman, 2016 ; Zhang et al, 2020 ), which interfered chicken’ accessibility to feed and water, predominantly reducing the growth and causing an economic loss in production.…”

Section: Discussionmentioning

confidence: 99%

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Tompkins¹,

Liu²,

Kim³

2023

Front. Physiol.

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The effects of hydrogen peroxide (H2O2) on the osteogenic differentiation of primary chicken mesenchymal stem cells (MSCs) were investigated. MSCs were subjected to an osteogenic program and exposed to various concentrations of H2O2 for 14 days. Results showed that high concentrations of H2O2 (200 and 400 nM) significantly increased pro-apoptotic marker CASP8 expression and impaired osteogenic differentiation, as indicated by decreased mRNA expression levels of osteogenesis-related genes and reduced in vitro mineralization. In contrast, long-term H2O2 exposure promoted basal expression of adipogenic markers at the expense of osteogenesis in MSCs during osteogenic differentiation, and increased intracellular reactive oxygen species (ROS) production, as well as altered antioxidant enzyme gene expression. These findings suggest that long-term H2O2-induced ROS production impairs osteogenic differentiation in chicken MSCs under an osteogenic program.

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In Sickness and in Health: The Oxygen Reactive Species and the Bone

Cited by 10 publications

References 135 publications

Dipeptidyl Peptidase 3 Activity as a Promising Biomarker of Bone Fragility in Postmenopausal Women

Dipeptidyl Peptidase 3 Activity as a Promising Biomarker of Bone Fragility in Postmenopausal Women

Allolobophora caliginosa coelomic fluid and extract alleviate glucocorticoid-induced osteoporosis in mice by suppressing oxidative stress and regulating osteoblastic/osteoclastic-related markers

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

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