Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin

Roy, Debashis

doi:10.1136/hrt.2004.049643

Cited by 206 publications

(159 citation statements)

References 6 publications

(5 reference statements)

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…1 Though the exact mechanism/stress mediator leading to the formation of IMA could be varied, in the laboratory the effect can be consistently repeated via exposure of albumin to hydroxyl radicals. 18 The significance of this assay in determining periods of ischemia is evident in that it has recently been licensed by the U.S. Food and Drug Administration as a rule-out test for cardiac ischemia. 25 Though the presence of IMA is not diagnostic of a cardiac infarct, as any regional ischemia has the potential to generate IMA, its absence indicates that it is most unlikely and patients with a low IMA score can be discharged.…”

Section: Discussionmentioning

confidence: 99%

“…There are several sites on the protein that have the ability to bind metals, though most interest to date has focused on the N-terminal region. 13,18 IMA was determined according to the cobalt-binding assay method of Bhagavan et al 13 Briefly, plasma was incubated with a cobalt chloride (1 g/L, 10 minutes) and dithiothreitol (1.5 g/L, 2 minutes) before dilution in saline prior to measurement at 470 nm in a spectrophotometer (Agilent 8453 Diode Array, Agilent, UK). IMA was calculated from the difference between samples measured with and without dithiothreitol.…”

Section: Measurement Of Albumin Functionmentioning

confidence: 99%

See 1 more Smart Citation

Alterations in the functional capacity of albumin in patients with decompensated cirrhosis is associated with increased mortality

Schnurr²,

et al. 2009

View full text Add to dashboard Cite

Albumin concentration is diminished in patients with liver failure. Albumin infusion improves survival of cirrhotic patients with spontaneous bacterial peritonitis, and it is hypothesized that this may be due in part to its detoxifying capabilities. The aim of this study was to perform detailed quantitative and qualitative assessment of albumin function in patients with cirrhosis. Healthy controls and patients with acute deterioration of cirrhosis requiring hospital admission (n ‫؍‬ 34) were included. Albumin function was assessed using affinity of the fatty acid binding sites using a spin label (16 doxyl-stearate) titration and electron paramagnetic resonance spectroscopy and ischemia-modified albumin (IMA) was measured. Twenty-two patients developed acute-on-chronic liver failure. Twelve were treated with the Molecular Adsorbents Recirculating System (MARS) and 10 with standard medical therapy. For each parameter measured, the patients' albumin had reduced functional ability, which worsened with disease severity. Fifteen patients died, and IMA, expressed as an albumin ratio (IMAR), was significantly higher in nonsurvivors compared with survivors (P < 0.001; area under the receiver operating curve ‫؍‬ A lbumin is the major plasma protein and constitutes around 50% of the cell free protein in healthy individuals. It is produced exclusively in the liver, and therefore its concentration is reduced during hepatic dysfunction. 1 Following the Cochrane meta-analysis describing potential harmfully effect of albumin infusion in critically ill patients, there has been a reexamination of the use of albumin infusions for volume replacement. However, the results of the recently published SAFE study have provided new data confirming the safety of albumin infusion in critically ill patients. 2,3 Liver failure results in multiple organ dysfunction, and mortality rates without liver transplantation remain unacceptably high. 4 However, recovery is associated with complete reversal of multiorgan dysfunction. At present, in patients with cirrhosis, albumin is used mainly to replenish the circulating volume. With increasing severity of cirrhosis, there is a progressive increase in cardiac output, which is associated with a progressive reduction in individual organ blood flow. This peculiar circulatory disturbance is thought to occur as a result of splanchnic

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Measurement Of Albumin Functionmentioning

confidence: 99%

Alterations in the functional capacity of albumin in patients with decompensated cirrhosis is associated with increased mortality

Schnurr²,

et al. 2009

View full text Add to dashboard Cite

show abstract

“…IMA levels are higher in many inflammatory and oxidative stress-associated diseases (Roy et al, 2006). Also, serum IMA levels increased in patients with gastric, prostate, soft tissue cancer and neuroblastoma (Mastella et al, 2009;Stachowicz-Stencel et al, 2011;Fidan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The generation of reactive oxygen species (ROS) and free radicals can transiently modify the N-terminal region of albumin and produce an increase in the concentration of IMA (Christenson et al, 2001;Roy et al, 2006). IMA may show a sensitive biochemical marker, especially for the diagnosis of myocardial ischemia (Sinha et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Ischemia Modified Albumin Levels and Oxidative Stress in Patients with Bladder Cancer

Elli̇dağ¹,

Eren²,

Aydın³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: Impaired oxidative/antioxidative status plays an important role in the pathogenesis of many diseases like cancer. The aim of this study was to evaluate the levels of the novel marker ischemia modified albumin (IMA) and albumin adjusted-IMA (Adj-IMA) in patients with bladder cancer (BC) as well as its association with total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI). Materials and Methods: Forty male patients with BC (mean age, 67.4±12 years) and forty age-sex matched healthy persons (mean age 56.0±1.7 years) were included in this study. Serum levels of IMA, TAS, TOS were analyzed and Adj-IMA and OSI was calculated. Results: Serum IMA, TOS and OSI values were significantly higher in patients with BC compared to controls (p<0.0001, p=0.01 and p=0.01, respectively), whereas TAS was significantly lower in BC patients (p=0.04). There was no significant difference for serum albumin-adjusted IMA levels between groups (p=0.4). Conclusions: In this study, it was found that there was an impaired oxidative/antioxidant status in favor of oxidative stress in BC patients. This observation was not confirmed by Adj-IMA calculation. There is no published report about serum concentrations of IMA in patients with BC. Further studies are needed to establish the relationship of IMA and oxidative stress parameters in BC and the significance of IMA to other cancers.

show abstract

“…It has been shown that the concentration of IMA increases within a couple of minutes from the onset of ischemia and remains elevated until 6-12 h and returns to its normal range after 24 h [11]. Recently, in vitro studies have shown that ROS, specifically the hydroxyl radical, may chemically modify human serum albumin, resulting in IMA formation [12].…”

Section: Introductionmentioning

confidence: 99%

Estimation of Ischemia Modified Albumin (IMA) Levels in Patients with Acute Coronary Syndrome

Gurumurthy¹,

Borra²,

Yeruva³

et al. 2013

Ind J Clin Biochem

View full text Add to dashboard Cite

Myocardial ischemia produces free radicals that catalyze a series of oxidative reactions that damage healthy tissues. The N-terminal sequence of albumin is one of the proteins modified by these highly reactive oxygen species and forms the ischemia modified albumin (IMA). This study involves investigations undertaken in different study groups to assess the levels of IMA. Mean serum IMA levels (U/mL) in patients with ST-segment elevated myocardial infarction (92.1 ± 10.6), non-ST-segment elevated myocardial infarction (87.3 ± 5.95) and unstable angina (UA) (88.9 ± 6.16) were significantly higher than noncardiac chest pain (77.9 ± 6.69) and also healthy subjects (54.7 ± 17.2) (p \ 0.001). IMA is a highly sensitive marker and has a high predictive value, which might prove the usefulness of this biomarker for early detection of myocardial ischemia. These data indicate a possible role of the IMA test in the early triage of patients with chest pain.

show abstract

Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin

Cited by 206 publications

References 6 publications

Alterations in the functional capacity of albumin in patients with decompensated cirrhosis is associated with increased mortality

Alterations in the functional capacity of albumin in patients with decompensated cirrhosis is associated with increased mortality

Ischemia Modified Albumin Levels and Oxidative Stress in Patients with Bladder Cancer

Estimation of Ischemia Modified Albumin (IMA) Levels in Patients with Acute Coronary Syndrome

Contact Info

Product

Resources

About