Role of Rabbit Lysozyme in In Vitro Serum and Plasma Serum Bactericidal Reactions Against
            <i>Bacillus subtilis</i>

Carroll, Stephen F.; Martı́nez, Rafael

doi:10.1128/iai.25.3.810-819.1979

Cited by 19 publications

(20 citation statements)

References 25 publications

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“…It should be noted that the effect of lysozyme in vivo is most probably potentiated by the presence of other factors, implying that the present data may tend to underestimate the importance of lysozyme. Variations in antibacterial activity of lysozymes from different sources have been reported elsewhere (Carroll & Martinez 1979;Iacono et al 1980). Such variations may indicate that some species have evolved more effective antimicrobial mechanisms than others.…”

Section: Discussionmentioning

confidence: 77%

Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens

Grinde¹

1989

Journal of Fish Diseases

211

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The antibacterial effect of two lysozyme variants purified from rainbow trout, Salmo gairdneri Riehardson, kidney was investigated as part of a project directed towards increasing the disease resistance of fish by the transgenie technique. Seven bacterial strains from five Gram-negative species, of which one was considered non-pathogenic, were examined. One of the rainbow trout lysozymes was surprisingly potent., having substantial antibacterial activity on all strains tested. Hen egg-white lysozyme was bactericidal only against the one species considered non-pathogenic. The data suggest that lysozyme docs play a role in the disease defence of rainbow trout and that the gene for the most aetive tysozyme may be suitable for testing the transgenie strategy.

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Section: Discussionmentioning

confidence: 77%

Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens

Grinde¹

1989

Journal of Fish Diseases

211

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“…Obtained results revealed that before infection with Salmonella, birds in T1 and T2 groups showed increase in lysozyme activity compared with control group. This finding may be due to increasing in intestinal lactic acid bacteria as mentioned by Carroll and Martinez (1979).…”

Section: Discussionmentioning

confidence: 82%

Influence of Feed Sanitation on Zootechnical Performance, Prevalence, Immune Status and Carcass Trait of Salmonella typhimurium Infected Broiler Chickens

El-Gha¹,

Tony²,

Mohamed³

2015

Asian J. of Animal Sciences

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An experiment was conducted to determine the effects of a commercial acidifier feed additive product containing formaldehyde on performance, Salmonella typhimurium prevalence and immune status of broiler chickens. Three hundreds, day-old chicks (Hubbard breed) were randomly distributed into three treatment groups (4 replicates each) using 25 chicks per replicate on floor pens. Control (C) birds were offered non-supplemented basal diets. Treatment groups 1 and 2 (T1 and T2) were fed diets containing product at 250 and 500 g tG 1 feed, respectively. Feed and water were offered ad-libitum for 35 days experimental period. Feed consumption and body weight were recorded weekly to calculate body weight gain and feed conversion. Blood samples were collected by time intervals to evaluate the immune status of the birds against some vaccines. At day 21 of age,20 birds were chosen randomly from each group (5 from each replicate) and were challenged orally with 1 mL containing 10 6 colony forming unit (CFU mLG 1) Salmonella typhimurium and were kept under observation for two weeks. At the end of the experimental period, 20 birds were chosen randomly from each group (5 from each replicate) to compare carcass yield. The results revealed that body weight gain was significantly (p<0.05) improved in chicks fed on diets containing product compared with the control one. The best feed conversions were recorded in T2. The results of the Salmonella typhimurium challenge experiment showed that both doses of the products significantly (p#0.05) reduced the signs, mortalities, gross lesions, shedding rate and re-isolation of Salmonella typhimurium. Dressing percentage and liver weight were non-significantly different between groups. Supplementation of the broiler diets significantly enhanced the immune responses measured against the vaccines used. It can be concluded that, using acidifiers and formaldehyde as feed additives for feed sanitation reflected positively on the zootechnical performance of broiler chickens, reduced the incidence of salmonellosis and enhanced the immune status of broiler chickens.

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“…Both approaches are fraught with difficulties. Bentonite is far from specific for the quantitative and sole removal of lysozyme (7,11,32), and the specific activities of different lysozymes are highly variable in the bacteriolytic and bactericidal reactions (3,30). To our knowledge, the only other reports using anti-human lysozyme antibody as a specific inhibitor for the human serum enzyme in these reactions were those of Glynn and Milne (11,12).…”

Section: Discussionmentioning

confidence: 99%

Sequential metabolic expressions of the lethal process in human serum-treated Escherichia coli: role of lysozyme

Martı́nez¹,

Carroll²

1980

Infect Immun

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Several metabolic parameters indicative of Escherichia coli function and integrity were kinetically examined in response to treatment with normal human serum in the presence and absence of functional human lysozyme. Specific inhibition of this enzyme in bacteriolytic and bactericidal reactions was accomplished by using purified rabbit anti-human lysozyme immunoglobulin G. Initiation of the complement-mediated alterations of cytoplasmic membrane integrity, as judged by the leakage of 86Rb from prelabeled cells or the hydrolysis of o-nitrophenyl-beta-D-galactopyranoside by a cryptic strain, was found to be independent of lysozyme action. Furthermore, inhibition of macromolecular synthesis by E. coli in response to serum treatment occurred at the same time regardless of the functional state of lysozyme. Although the rate and extent of bacteriolysis were reduced in the absence of lysozyme, the bactericidal kinetics was unaffected. These results demonstrate that the lethal events associated with the action of antibody and complement on gram-negative bacteria are independent of lysozyme, suggesting an accessory role for this enzyme in immune reactions. A possible temporal sequence of complement-induced effects occurring at the cell surface is presented.

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Role of Rabbit Lysozyme in In Vitro Serum and Plasma Serum Bactericidal Reactions Against Bacillus subtilis

Cited by 19 publications

References 25 publications

Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens

Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens

Influence of Feed Sanitation on Zootechnical Performance, Prevalence, Immune Status and Carcass Trait of Salmonella typhimurium Infected Broiler Chickens

Sequential metabolic expressions of the lethal process in human serum-treated Escherichia coli: role of lysozyme

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