Lysozyme from rainbow trout, Salmo gairdneri Richardson, as an antibacterial agent against fish pathogens

Abstract

The antibacterial effect of two lysozyme variants purified from rainbow trout, Salmo gairdneri Riehardson, kidney was investigated as part of a project directed towards increasing the disease resistance of fish by the transgenie technique. Seven bacterial strains from five Gram-negative species, of which one was considered non-pathogenic, were examined. One of the rainbow trout lysozymes was surprisingly potent., having substantial antibacterial activity on all strains tested. Hen egg-white lysozyme was bactericidal only against the one species considered non-pathogenic. The data suggest that lysozyme docs play a role in the disease defence of rainbow trout and that the gene for the most aetive tysozyme may be suitable for testing the transgenie strategy.

Effects of the “all-fish” growth hormone transgene expression on non-specific immune functions of common carp, Cyprinus carpio L.

“…Similarly, GH transgene expression also stimulated the phagocytosis of macrophages in head kidney (Table 1). As was mentioned previously, macrophages can produce lysozyme to kill pathogens, including the A. hydrophila used in the phagocytic assay (Grinde, 1989). Therefore, it can be inferred that the phagocytic activity promoted in the transgenics may correlate with their increased lysozyme activity.…”

Effect of Black Mustard (Brassica nigra) on the Interaction between Immune and Biotransformation Systems of Nile Tilapia (Oreochromis niloticus) Exposed to Benzo-a-Pyrene

“…In fish, lysozyme is recognized to be an opsonin and activates the complement system and phagocytes (Grinde, 1989). Thus, it plays an important role in the host defense mechanisms against infectious diseases (Lundblad et al, 1979;Lindsay, 1986;Lie et al, 1989).The results of this study revealed a significant increase in lysozyme activity in mustard extract group than the other groups.…”

Probiotics in Aquaculture of Kuwait - Current State and Prospect

“…Lysozyme has an antibiotic ability and is released by leukocytes. It can damage bacterial cell walls, especially of Grampositive and some Gram-negative bacteria (Grinde, 1989). Lysozyme activity varies between species of fish, genetic strains and different pathogens.…”