Role of Purine Metabolism Enzymes in Immune Pathogenesis of Fibro-Cavernous Pulmonary Tuberculosis

Евгеньевна, Дьякова Марина; Zhuravlev, Viacheslav; Эсмедляева, Д. С.; Perova, Tatiana

doi:10.15789/1563-0625-2016-1-85-90

Cited by 1 publication

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References 12 publications

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“…A total of 29 metabolites with between-group altered abundances were identi ed, including 41.8% In accordance with our ndings, Vitamin D combined with one amino acid, L-arginine, is reported to be a potential adjunctive immunotherapy in tuberculosis [28], and the administered dose mattered [29]. Purine metabolism has been correlated with cytokine production in patients with bro-cavernous pulmonary tuberculosis [30]. Fatty acid metabolism was reported to be in uenced by Vitamin D de ciency and consequently increased the risk of tuberculosis infection [31].…”

Section: Discussionsupporting

confidence: 74%

Calcitriol Exerts Prophylactic Anti-Mycobacterium Effect In A Dose-Dependent Manner

Gao

et al. 2021

Preprint

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Vitamin D was empirically applied for Tuberculosis (TB) treatment in the past, and is currently used as an adjuvant for TB therapy. Although an increasing pile of evidences suggests that vitamin D has no therapeutic effect against TB infection, the prophylactic effect of vitamin D in preventing TB remains largely undetermined. To experimentally valuate the potential prophylactic effect of calcitriol (the active form of vitamin D) against mycobacterium infection, we performed dose-gradient calcitriol soaking in 30-day-old zebrafish before Mycobacterium marinum (M. marinum) challenge through tail vein injection. 1H-NMR metabolomics analysis was further performed for illustration of potential mechanisms underlying the prophylactic effect of calcitriol against M. marinum. The results suggested that calcitriol exerts dose-dependent prophylactic anti-mycobacterium effects, i.e., the bacterial load and the corresponding inflammatory factors (IL-1β, TNF-α, and IFN-γ) expressions in M. marinum challenged zebrafish were reduced by low-dose (25 µg/L) or high-dose (2500 µg/L) calcitriol soaking, rather than by moderate-dose (250 µg/L) calcitriol soaking. Body weight of the M. marinum challenged zebrafish was recovered by high-dose prophylactic calcitriol soaking rather than by low-dose or moderate-dose calcitriol. The 1H-NMR metabolomic profiling identified 29 metabolites with altered abundance among the dose-gradient calcitriol groups, among which 22 metabolites were co-varied with the dose of calcitriol, the rest 7 metabolites were co-varied with the bacterial load and the inflammatory response in term of cytokine expression. Further pathway analysis indicated that the glycine, serine, and threonine metabolism pathway was the activated in both of the two metabolite groups, indicating that the pathway was altered by dose-gradient of calcitriol and was in response to M. marinum infection in zebrafish. The results of the present study suggested that the activation of glycine, serine and threonine metabolism pathway may play a potential role for the dose-dependent anti-mycobacterium effect induced by prophylactic calcitriol soaking.

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Section: Discussionsupporting

confidence: 74%

Calcitriol Exerts Prophylactic Anti-Mycobacterium Effect In A Dose-Dependent Manner

Gao

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Role of Purine Metabolism Enzymes in Immune Pathogenesis of Fibro-Cavernous Pulmonary Tuberculosis

Cited by 1 publication

References 12 publications

Calcitriol Exerts Prophylactic Anti-Mycobacterium Effect In A Dose-Dependent Manner

Calcitriol Exerts Prophylactic Anti-Mycobacterium Effect In A Dose-Dependent Manner

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