Role of protein synthesis in the protection conferred by ozone-oxidative-preconditioning in hepatic ischaemia/reperfusion

Abstract

Summary The liver is damaged by sustained ischaemia during liver transplantation, and the reperfusion after ischaemia results in further functional impairment. Ozone oxidative preconditioning (OzoneOP) protected the liver against ischaemia/reperfusion (I/R) injury through different mechanisms. The aim of this study was to investigate the influence of the inhibition of protein synthesis on the protective actions conferred by OzoneOP in hepatic I/R. Rats were treated with cycloheximide (CHX) in order to promote protein synthesis inhibition after OzoneOP treatment. Plasma transaminases, malondialdehyde and 4‐hydroxyalkenals and morphological characteristics were measured as an index of hepatocellular damage; Cu/Zn‐superoxide dismutase (SOD), Mn‐SOD, catalase, total hydroperoxides and glutathione levels as markers of endogenous antioxidant system. OzoneOP increased Mn‐SOD isoform and ameliorated mitochondrial damage. CHX abrogated the protection conferred by OzonoOP and decreased Mn‐SOD activity. Cellular redox balance disappeared when CHX was introduced. Protein synthesis is involved in the protective mechanisms mediated by OzoneOP. Ozone treatment preserved mitochondrial functions and cellular redox balance.

Preconditioning with ozone/oxygen mixture induces reversion of some indicators of oxidative stress and prevents organic damage in rats with fecal peritonitis

“…Recently, it was reported that OOP is able to afford protection against injury associated with hepatic ischemiareperfusion and cellular damage mediated by free radicals [19][20][21][22]. These authors have provided evidence that ozone may induce an adaptation to oxidative stress and also prepare the host to face physiopathological conditions mediated by ROS.…”

Ozone Oxidative Preconditioning Protects the Rat Kidney from Reperfusion Injury: The Role of Nitric Oxide

“…Moreover, ozone could prepare the host to face physiopathological events mediated by reactive oxygen species (ROS). It has been demonstrated that ozone induced protective effects in renal and hepatic Ischemia/reperfusion (I/R) injury [3][4][5][6][7]. This phenomenon was called ozone oxidative preconditioning (OzoneOP).…”

Protective effects of medical ozone combined with traditional Chinese medicine against chemically-induced hepatic injury in dogs

“…It has been used in an empirical fashion in recent years for therapy of viral hepatitis. Rectal insufflation with ozone can reduce hepatic and renal ischemia-reperfusion injury [10][11][12][13][14][15] . Yigan Fuzheng Paidu Capsules (YC), an empirical formula, is a compound preparation of traditional Chinese medicine (TCM) for treating chronic viral hepatitis.…”