Role of protein deimination in cardiovascular diseases: potential new avenues for diagnostic and prognostic biomarkers

Li-qun, Mao; Mostafa, Rowann; Ibili, Esra; Fert-Bober, Justyna

doi:10.1080/14789450.2021.2018303

Cited by 6 publications

(4 citation statements)

References 167 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous results have shown that H3cit is not able to cause cellular damage associated ETosis events [ 39 ]. In order to know the molecular mechanisms activated by citrullinated histones in those patients, an in vitro model of endothelial cells was used, since the endothelium is the first tissue in contact with histones when they are released into the bloodstream.…”

Section: Resultsmentioning

confidence: 99%

Histone Citrullination Mediates a Protective Role in Endothelium and Modulates Inflammation

Osca-Verdegal

Beltrán-García²,

Paes

et al. 2022

Cells

View full text Add to dashboard Cite

NETosis is a key host immune process against a pathogenic infection during innate immune activation, consisting of a neutrophil “explosion” and, consequently, NET formation, containing mainly DNA, histones, and other nuclear proteins. During sepsis, an exacerbated immune host response to an infection occurs, activating the innate immunity and NETosis events, which requires histone H3 citrullination. Our group compared the circulating histone levels with those citrullinated H3 levels in plasma samples of septic patients. In addition, we demonstrated that citrullinated histones were less cytotoxic for endothelial cells than histones without this post-translational modification. Citrullinated histones did not affect cell viability and did not activate oxidative stress. Nevertheless, citrullinated histones induced an inflammatory response, as well as regulatory endothelial mechanisms. Furthermore, septic patients showed elevated levels of circulating citrullinated histone H3, indicating that the histone citrullination is produced during the first stages of sepsis, probably due to the NETosis process.

show abstract

Section: Resultsmentioning

confidence: 99%

Histone Citrullination Mediates a Protective Role in Endothelium and Modulates Inflammation

Osca-Verdegal

Beltrán-García²,

Paes

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…Citrullinated histones appear to be a good option as they are directly involved in NETosis. Citrullinated histone H3 in combination with DNA and other NETosis-related markers has been shown to be a suitable analyte for prognosis in cancer, sepsis, or cardiovascular conditions [ 213 , 214 ]. In this context, the formulation and continuous refinement of a guideline based on consensus, comparable to the MISEV guidelines for EVs, would be a valuable aid for the further development of NET-based diagnostics [ 215 ].…”

Section: Net- and Neutrophil Ev-based Diagnosticsmentioning

confidence: 99%

Neutrophil Extracellular Traps and Neutrophil-Derived Extracellular Vesicles: Common Players in Neutrophil Effector Functions

Pfister

2022

Diagnostics

View full text Add to dashboard Cite

Neutrophil granulocytes are a central component of the innate immune system. In recent years, they have gained considerable attention due to newly discovered biological effector functions and their involvement in various pathological conditions. They have been shown to trigger mechanisms that can either promote or inhibit the development of autoimmunity, thrombosis, and cancer. One mechanism for their modulatory effect is the release of extracellular vesicles (EVs), that trigger appropriate signaling pathways in immune cells and other target cells. In addition, activated neutrophils can release bactericidal DNA fibers decorated with proteins from neutrophil granules (neutrophil extracellular traps, NETs). While NETs are very effective in limiting pathogens, they can also cause severe damage if released in excess or cleared inefficiently. Since NETs and EVs share a variety of neutrophil molecules and initially act in the same microenvironment, differential biochemical and functional analysis is particularly challenging. This review focuses on the biochemical and functional parallels and the extent to which the overlapping spectrum of effector molecules has an impact on biological and pathological effects.

show abstract

“…Hypercitrullination of MMP-9 results in a higher affinity for MMP-9 gelatin compared to control MMP-9 [211]. Further, the association of PAD-mediated citrullination of fibronectin, an important constituent of ECM, with CVDs, fibrosis, carcinogenesis, rheumatoid arthritis, alteration of integrin clustering, and focal adhesion stability suggests its role in regulating vascular remodeling because fibronectin-mediated inflammatory signaling through integrin α5 is important for vascular remodeling [212][213][214].…”

Section: Regulation Of Ecm Components By Ncrnasmentioning

confidence: 99%

Non-Coding RNAs in Regulating Plaque Progression and Remodeling of Extracellular Matrix in Atherosclerosis

Singh

Rai

Agrawal

2022

IJMS

View full text Add to dashboard Cite

Non-coding RNAs (ncRNAs) regulate cell proliferation, migration, differentiation, inflammation, metabolism of clinically important biomolecules, and other cellular processes. They do not encode proteins but are involved in the regulatory network of various proteins that are directly related to the pathogenesis of diseases. Little is known about the ncRNA-associated mechanisms of atherosclerosis and related cardiovascular disorders. Remodeling of the extracellular matrix (ECM) is critical in the pathogenesis of atherosclerosis and related disorders; however, its regulatory proteins are the potential subjects to explore with special emphasis on epigenetic regulatory components. The activity of regulatory proteins involved in ECM remodeling is regulated by various ncRNA molecules, as evident from recent research. Thus, it is important to critically evaluate the existing literature to enhance the understanding of nc-RNAs-regulated molecular mechanisms regulating ECM components, remodeling, and progression of atherosclerosis. This is crucial since deregulated ECM remodeling contributes to atherosclerosis. Thus, an in-depth understanding of ncRNA-associated ECM remodeling may identify novel targets for the treatment of atherosclerosis and other cardiovascular diseases.

show abstract

Role of protein deimination in cardiovascular diseases: potential new avenues for diagnostic and prognostic biomarkers

Cited by 6 publications

References 167 publications

Histone Citrullination Mediates a Protective Role in Endothelium and Modulates Inflammation

Histone Citrullination Mediates a Protective Role in Endothelium and Modulates Inflammation

Neutrophil Extracellular Traps and Neutrophil-Derived Extracellular Vesicles: Common Players in Neutrophil Effector Functions

Non-Coding RNAs in Regulating Plaque Progression and Remodeling of Extracellular Matrix in Atherosclerosis

Contact Info

Product

Resources

About