Role of Potassium Channels in the Effects of Hydrogen Sulfide on Contractility of Gastric Smooth Muscle Cells in Rats

Shaidullov, Ilnar; Shafigullin, M. U.; Gabitova, Liliia; Ситдиков, Ф. Г.; Зефиров, А. Л.; Ситдикова, Г. Ф.

doi:10.1134/s0022093018050083

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…The colon segments 5–7 mm length were fixed along the mesenteric border with stainless clips and placed in an organ chamber. The contractile power of isolated colon segments was studied under isometric conditions using a 4-channel isolated organ bath system (Biopac Inc., Goleta, CA, USA) according to our previous study [ 30 , 31 ]. The specimen was suspended upright in the bath (20 ml) with the upper part connected to the tensometric force transducer (TSD125C, Biopac Inc., Goleta, CA, USA) and the lower part fixed on a fastened hook.…”

Section: Methodsmentioning

confidence: 99%

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

et al. 2021

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Background Irritable bowel syndrome (IBS) is defined as a multifactorial disorder associated with visceral hypersensitivity, altered gut motility and dysfunction of the brain-gut axis. Gut microbiota and its metabolites are proposed as possible etiological factors of IBS. Short chain fatty acids (SCFAs) induce both inhibitory and stimulatory action on colon motility, however, their effects on the IBS model were not investigated. The aim of our study was to investigate the level of SFCAs in feces and their effects on colon motility in a mouse model of IBS. Methods IBS model was induced in mice by intracolonic infusion of 1% acetic acid during the early postnatal period. Mice colon hypersensitivity was assessed by the threshold of the abdominal withdrawal reflex in response to colorectal distention. Colon contractility was studied using proximal colon specimens in isometric conditions. Transit rates were assessed by the pellet propulsion in the isolated colon. Concentrations of SCFAs in feces were measured using gas–liquid chromatography. Results The concentration of SCFAs in feces of IBS model mice was higher compared to the control group. Visceral sensitivity to colorectal distension and colonic transit rate were increased indicating IBS with predominant diarrhea. The frequency and amplitude of spontaneous contractions of proximal colon segments from IBS mice were higher, but carbachol induced contractions were lower compared to control. During acute application of SCFAs (sodium propionate, sodium acetate or butyric acid) dose-dependently (0.5–30 mM) decreased tonic tension, frequency and amplitude of spontaneous and carbachol-evoked contractions. In the mouse IBS group the inhibitory effects SCFAs on spontaneous and carbachol-evoked contractions were less pronounced. At the same time intraluminal administration of butyrate (5 mM) increased the transit rate in the colon of both groups, but its stimulatory effect was more pronounced in mouse IBS model group. Conclusion Our data indicate that the increased transit rate in the mouse IBS model group is associated with a disbalance of activating and inhibiting action of SCFAs due to chronically elevated SCFA levels, which may impact the pathogenesis of IBS with predominant diarrhea syndrome.

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Section: Methodsmentioning

confidence: 99%

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

et al. 2021

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“…Anti-inflammatory decreased serum level of TNF-α and IL-1β and expression of mRNA in gastric mucosa [5,15] inhibited production of TNF-α, IL-1β in LPS-stimulated macrophages in vivo and in vitro [44] reduced mRNA and protein expression of HIF-1α in gastric mucosa [5,15] increased IL-10 expression in macrophages via activation p38MAPK [87] supressed NF-κB pathway in gastric mucosa [21] decreased ERK1/2 kinase activity in T cells [88] induced activation of AnxA1 pathway [21] re reduced mRNA and protein expression of HIF-1α in gastric mucosa and supressed NF-κB pathway in gastric mucosa [15] involved in regulation of Th1, Th2, and Th17 lymphocyte differentiation, decrease of IL-17A content [50] Anti-oxidative caused Nrf-2 /HMOX-1pathway upregulation [11,18] inhibited the lipid peroxidation [2] decreased level of MDA and increased production of glutathione (GSH) [7,56] decreased level of MDA and modulated SOD activity [56,89] Vasodilatation increased gastric microcirculation via sGC on endogenous NO and CO biosynthesis-dependent manner [53,54,56] Increased gastric microcirculation via sGC with contribution of NO biosynthesis pathway and independently on endogenous H 2 S activity [40,42,43,54,56] dependent on activation of K ATP channels [90] dependent on activation of K ATP channels [91] HCO 3 secretion in duodenum increased [64] increased [62,64] Impact on gut microbiota caused the reconstitution of microbiota biofilm dysbiosis [69,72] found to be involved in CO/HMOX-1 pathway in cross-talk between the microbiota and the mucosal immune compartment [49]…”

Section: Beneficial Effects Of H 2 S and Comentioning

confidence: 99%

Synergisms, Discrepancies and Interactions between Hydrogen Sulfide and Carbon Monoxide in the Gastrointestinal and Digestive System Physiology, Pathophysiology and Pharmacology

2020

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Endogenous gas transmitters, hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) are important signaling molecules known to exert multiple biological functions. In recent years, the role of H2S, CO and NO in regulation of cardiovascular, neuronal and digestive systems physiology and pathophysiology has been emphasized. Possible link between these gaseous mediators and multiple diseases as well as potential therapeutic applications has attracted great attention from biomedical scientists working in many fields of biomedicine. Thus, various pharmacological tools with ability to release CO or H2S were developed and implemented in experimental animal in vivo and in vitro models of many disorders and preliminary human studies. This review was designed to review signaling functions, similarities, dissimilarities and a possible cross-talk between H2S and CO produced endogenously or released from chemical donors, with special emphasis on gastrointestinal digestive system pathologies prevention and treatment.

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Nitric oxide in the mechanisms of inhibitory effects of sodium butyrate on colon contractions in a mouse model of irritable bowel syndrome

Shaidullov,

Bouchareb,

Sorokina

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Role of Potassium Channels in the Effects of Hydrogen Sulfide on Contractility of Gastric Smooth Muscle Cells in Rats

Cited by 7 publications

References 30 publications

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

Synergisms, Discrepancies and Interactions between Hydrogen Sulfide and Carbon Monoxide in the Gastrointestinal and Digestive System Physiology, Pathophysiology and Pharmacology

Nitric oxide in the mechanisms of inhibitory effects of sodium butyrate on colon contractions in a mouse model of irritable bowel syndrome

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