Role of Polyamines in the Control of Cell Proliferation and Differentiation

Heby, Olle

doi:10.1111/j.1432-0436.1981.tb01123.x

Cited by 914 publications

(393 citation statements)

References 145 publications

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“…As polyamines are required for growth [30], and as both replicating stages of T. cruzi (the epimastigote in the insect gut and the amastigote in mammalian cells) are normally bathed in micromolar levels of diamines and polyamines, it could be argued that these stages do not require a capacity for de no o synthesis when they can simply take them up from their surroundings. Perhaps then the activity of these transporters plays a central role in the overall control of intracellular polyamine levels in T. cruzi in a similar fashion to that observed with mammalian ODC, the dominant controlling factor of their entire polyamine pathway [31].…”

Section: Discussionmentioning

confidence: 99%

Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes

Quesne

Fairlamb

1996

Biochemical Journal

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Trypanosoma cruzi epimastigotes take up exogenous [3H]putrescine and [3H]cadaverine by a rapid, high-affinity, transport system that exhibits saturable kinetics (putrescine Km 2.0 μM, Vmax 3.3 nmol/min per 108 cells; cadaverine Km 13.4 μM, Vmax 3.9 nmol/min per 108 cells). Putrescine transport is temperature dependent and requires the presence of a membrane potential and thiol groups for activity. Its activity is altered in response to extracellular putrescine levels and as the cells proceed through the growth cycle. This transporter shows high specificity for the diamines putrescine and cadaverine, but low specificity for the polyamines spermidine and spermine. The existence of rapid diamine/polyamine transport systems whose activity can be adjusted in response to the growth conditions is of particular importance, as they seem unable to synthesize their own putrescine [Hunter, Le Quesne and Fairlamb (1994) Eur. J. Biochem. 226, 1019–1027].

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Section: Discussionmentioning

confidence: 99%

Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes

Quesne

Fairlamb

1996

Biochemical Journal

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“…When cultures of wild type CHO or of mouse NIH3T3 fibroblast cells grow to stationary phase, ODC expression decreases to low, even undetectable levels [2]. Stimulation of quiescent cultures by serum or TPA causes ODC expression to increase.…”

Section: Resultsmentioning

confidence: 99%

“…Their function in cells, however, is not known. The level of ODC activity is finely tuned and directly correlated with cell proliferation [2]. Thus promotion of cell growth by mitogens, growth factors and oncogenic transformation induces ODC activity, whereas slowing of cell growth by contact inhibition or terminal differentiation decreases it.…”

Section: Introductionmentioning

confidence: 99%

Regulation of mouse ornithine decarboxylase activity by cell growth, serum and tetradecanoyl phorbol acetate is governed primarily by sequences within the coding region of the gene

1989

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To determine the genetic elements required for modulation of ornithine decarboxylase (ODC) activity in response to cell growth or treatment with serum or with tetradecanoyl phorbol acetate, ODCdeficient cells were transfected with a series of recombinant DNAs encoding mouse ODC. All of the transfected cells expressing an intact mouse ODC protein displayed regulation of ODC activity, including those expressing a construct deprived of all ODC-specific sequence information except the protein-coding region. ODC mRNA changed much less than enzymatic activity. A mutation of the protein-coding region that converted ODC from an unstable to a stable intracellular protein attenuated the regulatory response. We conclude that post-transcriptional events associated with ODC degradation dominate the response to these stimuli.

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“…This has led some researchers to suggest that polyamines mediate the action of plant hormones or are part of their signal response pathways [13]. In animal systems polyamine biosynthesis increases in response to hormones, and the polyamines produced are necessary for hormone action [14,15]. Since the early work of Slankis [16], phytohormones produced by the plant partner have been suspected to be involved in some of the morphological and/or anatomical modifications characteristic of the ectomycorrhizal association.…”

Section: Introductionmentioning

confidence: 99%

First‐report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment

et al. 1996

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DFMO (~-DL-difluoromethylornithine), a specific irreversible inhibitor of ornithine decarboxylase (ODC), a polyamine biosynthetic pathway enzyme, strongly inhibits root growth and arbuscular mycorrhizal infection of Pisum sativum (P56 myc +, isogenic mutant of cv. Frisson). This inhibition is reversed when exogenous polyamine (putrescine) is included in the DFMO treatment, showing that the effect of DFMO on arbuscular mycorrhizal infection is indeed due to putrescine limitation and suggesting that ODC may have a role in root growth and mycorrhizal infection. However, treatment with gibbereHic acid (GA3) which increased root titers of polyamines strongly inhibited arbuscular mycorrhizal development. The possible role of polyamines in the regulation of the development of arbuscular mycorrhizal infection is discussed.

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Role of Polyamines in the Control of Cell Proliferation and Differentiation

Cited by 914 publications

References 145 publications

Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes

Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes

Regulation of mouse ornithine decarboxylase activity by cell growth, serum and tetradecanoyl phorbol acetate is governed primarily by sequences within the coding region of the gene

First‐report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment

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