Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles

Ovais, Muhammad; Khalil, Ali Talha; Islam, Nazar Ul; Ahmad, Irshad; Ayaz, Muhammad; Saravanan, Muthupandian; Shinwari, Zabta Khan; Mukherjee, Sudip

doi:10.1007/s00253-018-9146-7

Cited by 280 publications

(120 citation statements)

References 112 publications

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“…5 In contrast, the biological method for the synthesis of AgNPs, called "green synthesis", is the most advantageous given that it is cost effective, ecologically correct, reproducible, and energy efficient. 6 In this context, the use of fungi in the green synthesis of NPs has been highlighted, as the process to synthesize and culture them is easier, and large scale synthesis is possible using a small quantity of biomass, making it economically feasible. 7,8 Additionally, filamentous fungi produce a large quantity of extracellular enzymes and organic compounds needed for NP green synthesis.…”

Section: Introductionmentioning

confidence: 99%

Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans

Barbosa¹,

Silva²,

Ferraz³

et al. 2019

IJN

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Background: Helminth parasites cause morbidity and mortality in both humans and animals. Most anthelmintic drugs used in the treatment of parasitic nematode infections act on target proteins or regulate the electrical activity of neurons and muscles. In this way, it can lead to paralysis, starvation, immune attack, and expulsion of the worm. However, current anthelmintics have some limitations that include a limited spectrum of activity across species and the threat of drug resistance, which highlights the need for new drugs for human and veterinary medicine. Purpose: Present study has been conducted to determine the anthelmintic activity of silver nanoparticles (AgNPs) synthesized from the extract of nematophagous fungus, Duddingtonia flagrans, on the infecting larvae of Ancylostoma caninum (L 3 ). Methods: The nanoparticles were characterized by visual, ultraviolet, Fourier-transform infrared spectroscopy, transmission electron microscopy (TEM) analysis, and X-ray diffraction. The in vitro study was based on experiments to inhibit the motility of infective larvae (L 3 ), and the ultrastructural analysis of the nematode was performed by images obtained by TEM. Results: The XRD studies revealed the crystalline nature of the nanoparticles, and FTIR results implied that AgNPs were successfully synthesized and capped with compounds present in the extract. The results showed that the green synthesis of AgNPs exhibited nematicidal activity, being the only ones capable of penetrating the cuticle of the larvae, causing changes in the tegmentum, and consequently, the death of the nematode. Conclusion: The extract of the fungus D. flagrans is able to synthesize AgNP and these have a nematicidal action.

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Section: Introductionmentioning

confidence: 99%

Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans

Barbosa¹,

Silva²,

Ferraz³

et al. 2019

IJN

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“…On the other hand, physical methods for synthesis of NPs are energy intensive. To counteract, green and environmentally friendly methods are proposed for the synthesis of NPs …”

Section: Introductionmentioning

confidence: 99%

Physiochemical properties and novel biological applications of Callistemon viminalis‐mediated α‐Cr₂O₃ nanoparticles

Hassan

Khalil

Solangi

et al. 2019

Applied Organom Chemis

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We report an eco-friendly synthesis of α-Cr 2 O 3 nanoparticles (NPs) using Callistemon viminalis (Bottle Brush) flower extracts as an efficient reducing and capping agent. NPs of sizes 15 nm and 17 nm were synthesized by annealing them at 400°C and 500°C, respectively, which were characterized by X-ray diffraction, UV-Vis, Fourier transform-infrared, high-resolution-transmission electron microscopy/scanning electron microscopy, SAED, energy-dispersive X-ray spectroscopy and SQUID. Microplate-based assay was used for examining antibacterial potential against 12 pathogenic bacterial strains, and their minimum inhibitory concentrations were calculated. MTT cytotoxic assay was accomplished on Leishmania tropica amastigotes and promastigotes, which revealed IC 50 values of 44 μg/ml and 10.56 μg/ml, respectively. An IC 50 value of 46.32 μg/ml was obtained for HepG2 cancer cells. Enzyme inhibition studies indicated good acetylcholinesterase, moderate butyrylcholinesterase and low alpha-glucosidase inhibition. Hemolytic assay indicated hemocompatibility at low concentration. In addition, good DPPH radical scavenging and moderate reducing power and total antioxidant potential was revealed by α-Cr 2 O 3 NPs.

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“…Medicinal plants are playing a significant role in drug discovery and development against numerous disorders [40][41][42][43][44]. The main advantage of medicinal plants as a source of new drugs is the molecular diversity of secondary metabolites in these herbs [45,46].…”

Section: Resultsmentioning

confidence: 99%

Benzoic Acid Derivatives of Ifloga spicata (Forssk.) Sch.Bip. as Potential Anti-Leishmanial against Leishmania tropica

et al. 2019

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This study aimed to appraise the anti-leishmanial potentials of benzoic acid derivatives, including methyl 3,4-dihydroxybenzoate (compound 1) and octadecyl benzoate (compound 2), isolated from the ethnomedicinally important plant Ifloga spicata (I. spicata). Chemical structures were elucidated via FT-IR, mass spectrometry, and multinuclear (1H and 13C) NMR spectroscopy. Anti-leishmanial potentials of the compounds were assessed using Leishmania tropica promastigotes. Moreover, acridine orange fluorescent staining was performed to visualize the apoptosis-associated changes in promastigotes under a fluorescent microscope. A SYTOX assay was used to check rupturing of Leishmania promastigote cell membranes using 0.1% Triton X-100 as positive control. A DNA interaction assay was carried out to assess DNA attachment potential. AutoDock software was used to check the binding affinity of compounds with surface enzyme leishmanolysin gp63 (1LML). Both compounds exhibited considerable anti-leishmanial potential, with LD50 values of 10.40 ± 0.09 and 14.11 ± 0.11 μg/mL for compound 1 and compound 2, respectively. Both compounds showed higher binding affinity with the leishmanolysin (gp63) receptor/protease of Leishmania, as assessed using computational analysis. The binding scores of compounds 1 and 2 with target gp63 were −5.3 and −5.6, respectively. The attachment of compounds with this receptor resulted in their entry into the cell where they bound with Leishmania DNA, causing apoptosis. The results confirmed that the investigated compounds have anti-leishmanial potential and are potential substitutes as natural anti-leishmanial agents against L. tropica.

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Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles

Cited by 280 publications

References 112 publications

<p>Nematicidal activity of silver nanoparticles from the fungus <em>Duddingtonia flagrans</em></p>

<p>Nematicidal activity of silver nanoparticles from the fungus <em>Duddingtonia flagrans</em></p>

Physiochemical properties and novel biological applications of Callistemon viminalis‐mediated α‐Cr₂O₃ nanoparticles

Benzoic Acid Derivatives of Ifloga spicata (Forssk.) Sch.Bip. as Potential Anti-Leishmanial against Leishmania tropica

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Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles

Cited by 280 publications

References 112 publications

<p>Nematicidal activity of silver nanoparticles from the fungus <em>Duddingtonia flagrans</em></p>

<p>Nematicidal activity of silver nanoparticles from the fungus <em>Duddingtonia flagrans</em></p>

Physiochemical properties and novel biological applications of Callistemon viminalis‐mediated α‐Cr2O3 nanoparticles

Benzoic Acid Derivatives of Ifloga spicata (Forssk.) Sch.Bip. as Potential Anti-Leishmanial against Leishmania tropica

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Physiochemical properties and novel biological applications of Callistemon viminalis‐mediated α‐Cr₂O₃ nanoparticles