Role of MUC1 in lubrication of pleural mesothelial cells cultured on fibrine gel

Bodega, Francesca; Sironi, Chiara; Zocchi, Luciano; Porta, Cristina

doi:10.1016/j.tice.2021.101503

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…The mean value of µ was 0.0175 ± 0.001. This value was similar to those measured on pleural tissue specimens (approximately 0.025) [19], and on mesothelial cells in vitro (0.021 ± 0.001 and 0.032 ± 0.002 in 4/4 RM-4 and CARM-L1 TG3 cell line, respectively) [13]. Chen et al [14] found that the surface sliding friction of endothelial cell monolayers cultured on soft PNaSS gel was much greater than that exerted on the walls of blood vessels in vivo.…”

Section: Discussionsupporting

confidence: 88%

“…Aprotinin, a known serine protease inhibitor, can be used to prevent the breakdown of fibrin [17,24]. In a previous study, in which we measured the µ of mesothelial cells cultured on fibrin gel [13], we added aprotinin to the medium. Nevertheless, the present findings indicated that, since, for friction measurements, gels are generally used within 4-7 days, the addition of aprotinin to the medium is not necessary.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous studies, we performed such measurements on ex vivo tissue samples [9][10][11], using the apparatus built to measure frictional force described by D'Angelo et al [12]. This apparatus was adapted to perform measurements on cell monolayers [13]. When cells cultured on a hard substrate slide against each other under normal stress, they are easily destroyed due to stress concentration.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Bodega

Sironi

Zocchi

et al. 2022

IJMS

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To study the friction of cell monolayers avoiding damage due to stress concentration, cells can be cultured on fibrin gels, which have a structure and viscoelasticity similar to that of the extracellular matrix. In the present research, we studied different gel compositions and surface coatings in order to identify the best conditions to measure friction in vitro. We examined the adhesion and growth behavior of mesothelial cell line MET-5A on fibrin gels with different fibrinogen concentrations (15, 20, and 25 mg/mL) and with different adhesion coatings (5 μg/mL fibronectin, 10 μg/mL fibronectin, or 10 μg/mL fibronectin + 10 μg/mL collagen). We also investigated whether different substrates influenced the coefficient of friction and the ability of cells to stick to the gel during sliding. Finally, we studied the degradation rates of gels with and without cells. All substrates tested provided a suitable environment for the adherence and proliferation of mesothelial cells, and friction measurements did not cause significant cell damage or detachment. However, in gels with a lower fibrinogen concentration, cell viability was higher and cell detachment after friction measurement was lower. Fibrinolysis was negligible in all the substrates tested.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Bodega

Sironi

Zocchi

et al. 2022

IJMS

Self Cite

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“…An in vivo methodology was developed to investigate the tactile friction and sensory properties between human skin and hair, and effects of hair care products/shampoos were evaluated [766]. A wide range of tribological problems were examined, including in instruments [767], urinary catheter [768], rectal sexually transmitted infections [769], pleural mesothelial cells [770], gastroscope device-esophagus interface [771], cochlear [772], etc. The effective tribological mechanisms of animals and consequent bionics designs were investigated.…”

Section: Othersmentioning

confidence: 99%

A review of advances in tribology in 2020–2021

Meng

et al. 2022

Friction

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Around 1,000 peer-reviewed papers were selected from 3,450 articles published during 2020–2021, and reviewed as the representative advances in tribology research worldwide. The survey highlights the development in lubrication, wear and surface engineering, biotribology, high temperature tribology, and computational tribology, providing a show window of the achievements of recent fundamental and application researches in the field of tribology.

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Exploring the role of the MUC1 mucin in human oral lubrication by tribological in vitro studies

Ammam,

Pailler-Mattéi,

Ouillon

et al. 2024

Preprint

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In the context of the oral cavity, an organic layer known as the mucosal pellicle (MP) adheres to the surface of the oral epithelium, playing a pivotal role in lubricating and safeguarding oral tissues. The formation of the MP is driven by interactions between a transmembrane mucin known as MUC1, located on the oral epithelium, and salivary secreted mucin, namely MUC5B and MUC7. This study aimed to investigate the function of MUC1 and the influence of its structure on MP lubrication properties. We proposed a novel methodology to study oral lubrication based on four different models of oral epithelium on which we conducted in vitro tribological studies. These models expressed varying forms of MUC1, each possessing on of the distinct domain constituting the mucin. Mechanical parameters were used as indicators of lubrication efficiency and, consequently, of the role played by MUC1 in oral lubrication. The results from the tribological tests revealed that the presence of full MUC1 resulted in enhanced lubrication. Furthermore, the structure of MUC1 protein drive the lubrication. In conclusion, the mechanical tests conducted on our epithelium models demonstrated that MUC1 actively participates in epithelium lubrication by facilitating the formation of the MP.

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Role of MUC1 in lubrication of pleural mesothelial cells cultured on fibrine gel

Cited by 3 publications

References 33 publications

Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

A review of advances in tribology in 2020–2021

Exploring the role of the MUC1 mucin in human oral lubrication by tribological in vitro studies

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