Role of Msi1 and PYGO2 in esophageal squamous cell carcinoma depth of invasion

Moghbeli, Meysam; Sadrizadeh, Ali; Forghanifard, Mohammad Mahdi; Mozaffari, Hooman Mosannen; Golmakani, Ebrahim; Abbaszadegan, Mohammad Reza

doi:10.1007/s12079-015-0314-6

Cited by 27 publications

(20 citation statements)

References 26 publications

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“…The present study was in line with our previous projects about EGFR and MSI1 in ESCC patients to find a probable correlation between these markers and introduce a panel of diagnostic markers [14, 25, 26, 28–30]. We have recently reported a direct correlation between PYGO2, the main transcription factor of the WNT pathway, and EGFR, one of the target genes in the WNT pathway [25].…”

Section: Discussionsupporting

confidence: 87%

WNT and NOTCH signaling pathways as activators for epidermal growth factor receptor in esophageal squamous cell carcinoma

et al. 2018

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BackgroundEsophageal squamous cell carcinoma (ESCC) is the most common histological type of esophageal cancer, with a poor prognosis. Deregulation of WNT and NOTCH signaling pathways is important in ESCC progression, which can be due to either malfunction of their components or crosstalk with other pathways. Therefore, identification of new crosstalk between such pathways may be effective to introduce new strategies for targeted therapy of cancer. A correlation study was performed to assess the probable interaction between growth factor receptors and WNT/NOTCH pathways via the epidermal growth factor receptor (EGFR) and Musashi1 (MSI1), respectively.MethodsLevels of MSI1/EGFR mRNA expression in tumor tissues from 48 ESCC patients were compared to their corresponding normal tissues using real-time polymerase chain reaction.ResultsThere was a significant correlation between EGFR and MSI1 expression (p = 0.05). Moreover, there was a significant correlation between EGFR/MSI1 expression and grade of tumor differentiation (p = 0.02).ConclusionThis study confirms a direct correlation between MSI1 and EGFR and may support the important role of MSI1 in activation of EGFR through NOTCH/WNT pathways in ESCC.

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Section: Discussionsupporting

confidence: 87%

WNT and NOTCH signaling pathways as activators for epidermal growth factor receptor in esophageal squamous cell carcinoma

et al. 2018

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“…Relevant studies revealed that CD271, CD133 and CD90 affected CSC development in ESCC, however, we are the first to propose that Msi1, a well known molecule regulating cell division, plays a significant role in ESCC development by promoting proliferation and decreasing apoptosis of cancer cells. Firstly, as revealed in other studies, Msi1 had a higher expression in ESCC tumor tissues compared with adjacent non-cancerous tissues and its distribution was also more diffuse ( 32 , 33 ). As suggested in gastric cancer ( 34 ), due to the difference in proliferation patterns between cancer cells and normal cells, Msi1 had a different expression in cancer and normal matched tissues.…”

Section: Discussionmentioning

confidence: 62%

Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

et al. 2017

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Esophageal cancer ranks as the sixth leading cause of cancer-related deaths worldwide. Cancer stemness is mainly considered to be the key factor for cancer recurrence particularly in esophageal cancer. It is important to identify cancer stem cell markers as targets in future therapies. The present study aimed to investigate the expression of putative cancer stem cell-related marker musashi1 (Msi1) and assess the correlation with clinicopathologcal status of esophageal squamous cell carcinoma (ESCC) cases. We then clarified the role of Msi1 in esophageal cancer cells during proliferation, apoptosis, sphere formation and migration. Finally, we investigated the relationship of Msi1 with the prognosis of ESCC patients. ESCC tissue samples from 93 patients and 20 paired histologically normal tissues were procured for immunohistochemical analysis. We analyzed the characteristics of Msi1, using sphere formation and anchorage independent growth. Moreover, using flow cytometry and Cell Counting Kit-8 (CCK-8) assay, we investigated the role of Msi1 in cancer cell proliferation and apoptosis. Furthermore, we clarified the role of Msi1 in the process of sphere formation and migration of ESCC cells through knockdown of Msi1 expression by siRNA in ESCC cell lines. The results revealed that there was a higher expression of Msi1 in ESCC specimens compared with normal tissues. In addition, Msi1 expression was significantly associated with clinical stage and lymph node metastasis. Most importantly, the increased immunocytochemical staining of Msi1 in spheroid cells revealed the stemness characteristics of Msi1 in ESCC. In addition, we found that silencing of Msi1 decreased cell proliferation, migration and induced apoptosis in TE-7 and KYSE70 cells. Furthermore, downregulation of Msi1 attenuated the sphere formation ability of ESCC cells. Patients with higher expression of Msi1 had a shorter survival. In conclusion, Msi1 acts as a stemness-associated gene in esophageal cancer cell lines and could serve as a prognostic marker in patients with ESCC.

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“…36,37 PYG02 is closely related to the prognosis of glioma, esophageal squamous cell carcinoma, hepatocellular carcinoma and other tumors. [38][39][40] CTNNA1 is closely related to the prognosis of invasive breast cancer and renal cell carcinoma. 41,42 PTPRK is a marker of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

<p>Gene Expression Along with Genomic Copy Number Variation and Mutational Analysis Were Used to Develop a 9-Gene Signature for Estimating Prognosis of COAD</p>

Lu¹,

Wu²,

Cui³

et al. 2020

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This study aims to systematically analyze multi-omics data to explore new prognosis biomarkers in colon adenocarcinoma (COAD). Materials and Methods: Multi-omics data of COAD and clinical information were obtained from The Cancer Genome Atlas (TCGA). Univariate Cox analysis was used to select genes which significantly related to the overall survival. GISTIC 2.0 software was used to identify significant amplification or deletion. Mutsig 2.0 software was used to identify significant mutation genes. The 9-gene signature was screened by random forest algorithm and Cox regression analysis. GSE17538 dataset was used as an external dataset to verify the predictive ability of 9-gene signature. qPCR was used to detect the expression of 9 genes in clinical specimens. Results: A total of 71 candidate genes are obtained by integrating genomic variation, mutation and prognostic data. Then, 9-gene signature was established, which includes HOXD12, RNF25, CBLN3, DOCK3, DNAJB13, PYGO2, CTNNA1, PTPRK, and NAT1. The 9-gene signature is an independent prognostic risk factor for COAD patients. In addition, the signature shows good predicting performance and clinical practicality in training set, testing set and external verification set. The results of qPCR based on clinical samples showed that the expression of HOXD12, RNF25, CBLN3, DOCK3, DNAJB13, and PYGO2 was increased in colon cancer tissues and the expression of CTNNA1, PTPRK, NAT1 was decreased in colon cancer tissues. Conclusion: In this study, 9-gene signature is constructed as a new prognostic marker to predict the survival of COAD patients.

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Role of Msi1 and PYGO2 in esophageal squamous cell carcinoma depth of invasion

Cited by 27 publications

References 26 publications

WNT and NOTCH signaling pathways as activators for epidermal growth factor receptor in esophageal squamous cell carcinoma

WNT and NOTCH signaling pathways as activators for epidermal growth factor receptor in esophageal squamous cell carcinoma

Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

<p>Gene Expression Along with Genomic Copy Number Variation and Mutational Analysis Were Used to Develop a 9-Gene Signature for Estimating Prognosis of COAD</p>

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