Role of Msi1 and MAML1 in Regulation of Notch Signaling Pathway in Patients with Esophageal Squamous Cell Carcinoma

Moghbeli, Meysam; Forghanifard, Mohammad Mahdi; Sadrizadeh, Ali; Mozaffari, Hooman Mosannen; Golmakani, Ebrahim; Abbaszadegan, Mohammad Reza

doi:10.1007/s12029-015-9753-9

Cited by 29 publications

(15 citation statements)

References 28 publications

(37 reference statements)

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“…Relevant studies revealed that CD271, CD133 and CD90 affected CSC development in ESCC, however, we are the first to propose that Msi1, a well known molecule regulating cell division, plays a significant role in ESCC development by promoting proliferation and decreasing apoptosis of cancer cells. Firstly, as revealed in other studies, Msi1 had a higher expression in ESCC tumor tissues compared with adjacent non-cancerous tissues and its distribution was also more diffuse ( 32 , 33 ). As suggested in gastric cancer ( 34 ), due to the difference in proliferation patterns between cancer cells and normal cells, Msi1 had a different expression in cancer and normal matched tissues.…”

Section: Discussionmentioning

confidence: 62%

Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

et al. 2017

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Esophageal cancer ranks as the sixth leading cause of cancer-related deaths worldwide. Cancer stemness is mainly considered to be the key factor for cancer recurrence particularly in esophageal cancer. It is important to identify cancer stem cell markers as targets in future therapies. The present study aimed to investigate the expression of putative cancer stem cell-related marker musashi1 (Msi1) and assess the correlation with clinicopathologcal status of esophageal squamous cell carcinoma (ESCC) cases. We then clarified the role of Msi1 in esophageal cancer cells during proliferation, apoptosis, sphere formation and migration. Finally, we investigated the relationship of Msi1 with the prognosis of ESCC patients. ESCC tissue samples from 93 patients and 20 paired histologically normal tissues were procured for immunohistochemical analysis. We analyzed the characteristics of Msi1, using sphere formation and anchorage independent growth. Moreover, using flow cytometry and Cell Counting Kit-8 (CCK-8) assay, we investigated the role of Msi1 in cancer cell proliferation and apoptosis. Furthermore, we clarified the role of Msi1 in the process of sphere formation and migration of ESCC cells through knockdown of Msi1 expression by siRNA in ESCC cell lines. The results revealed that there was a higher expression of Msi1 in ESCC specimens compared with normal tissues. In addition, Msi1 expression was significantly associated with clinical stage and lymph node metastasis. Most importantly, the increased immunocytochemical staining of Msi1 in spheroid cells revealed the stemness characteristics of Msi1 in ESCC. In addition, we found that silencing of Msi1 decreased cell proliferation, migration and induced apoptosis in TE-7 and KYSE70 cells. Furthermore, downregulation of Msi1 attenuated the sphere formation ability of ESCC cells. Patients with higher expression of Msi1 had a shorter survival. In conclusion, Msi1 acts as a stemness-associated gene in esophageal cancer cell lines and could serve as a prognostic marker in patients with ESCC.

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Section: Discussionmentioning

confidence: 62%

Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

et al. 2017

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“…Reproduced from the Cancer Genome Atlas (TCGA) database, the network showed interactions between Notch genes calculated by genetic alteration. strongly associated with cell differentiation and cell fate determination indicated that increased MAML1 activity could promote the malignant potential of cells, such as in breast and esophageal cancer [29,30].…”

Section: Discussionmentioning

confidence: 99%

Genetic alteration in notch pathway is associated with better prognosis in renal cell carcinoma

et al. 2015

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Notch signaling was associated with a variety of cancers but was not comprehensively studied in clear‐cell renal cell carcinoma (ccRCC). We have in this study studied the genetic alteration (mutation and copy number variance) of Notch gene set in the Cancer Genome Atlas (TCGA) Kidney Renal Clear Cell Carcinoma (KIRC) database. We found that Notch pathway was frequently altered in ccRCC. The Notch gene set was genetically altered in 182 (44%) of the 415 ccRCC patients. CNV was the predominant type of alteration in most genes. Alterations in KAT2B and MAML1 occurred in 13% and 19% of patients, respectively, both of which were functionally active in ccRCC. Deletion of VHL was exclusively found in cases with Notch alteration. Overall survival was longer in ccRCC patients with altered‐Notch pathway. The median survival was 90.41 months in Notch‐altered cases and 69.15 in Notch‐unaltered cases (P = 0.0404). The median disease free time was 89.82 months in Notch‐altered cases and 77.27 months in in Notch‐unaltered cases (P = 0.935). Conclusively, Notch signaling was altered in almost half of the ccRCC patients and copy number variances in MAML1 and KAT2B were predominant changes. These findings broadened our understanding of the role of Notch in ccRCC. © 2015 BioFactors, 42(1):41–48, 2016

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“…This pathway is a cell to cell contact process which is activated via the ligand binding with a family of trans membrane receptors (NOTCH1-4). Ligand binding releases the intracellular domain of Notch (ICN) into the cytoplasm which eventually enters to the nucleus where it regulated the expression of NOTCH target genes [217]. Levels of NOTCH1 mRNA expression as one of the NOTCH receptors was assessed in BC patients and showed a significant increase in invasive ductal types compared with other histopathological types.…”

Section: Main Textmentioning

confidence: 99%

Genetic and molecular biology of breast cancer among Iranian patients

Moghbeli

2019

J Transl Med

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Background, Breast cancer (BC) is one of the leading causes of cancer related deaths in Iran. This high ratio of mortality had a rising trend during the recent years which is probably associated with late diagnosis. Main body Therefore it is critical to define a unique panel of genetic markers for the early detection among our population. In present review we summarized all of the reported significant genetic markers among Iranian BC patients for the first time, which are categorized based on their cellular functions. Conclusions This review paves the way of introducing a unique ethnic specific panel of diagnostic markers among Iranian BC patients. Indeed, this review can also clarify the genetic and molecular bases of BC progression among Iranians.

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Role of Msi1 and MAML1 in Regulation of Notch Signaling Pathway in Patients with Esophageal Squamous Cell Carcinoma

Abstract: We showed that there is not any direct correlation and feedback between the Msi1 and MAML1 in ESCC patients.

Cited by 29 publications

References 28 publications

Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

Musashi1, a potential prognostic marker in esophageal squamous cell carcinoma

Genetic alteration in notch pathway is associated with better prognosis in renal cell carcinoma

Genetic and molecular biology of breast cancer among Iranian patients

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