Role of miR-647 in human gastric cancer suppression

Cao, Wenlong; Wei, Weiyuan; Zhan, Zexu; Xie, Dongyi; Xie, Yubo; Xiao, Qiang

doi:10.3892/or.2017.5383

Cited by 35 publications

(33 citation statements)

References 40 publications

(39 reference statements)

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“…Moreover, we found that the cell invasion ability of glioma cells was suppressed by miR‐647 mimic. In summary, the results of the present study indicated that miR‐647 may function as a tumor suppressive role in glioma, which is consistent with its role in non‐small cell lung cancer or gastric cancer . A recent study reported that miR‐647 expression can be regulated by long non‐coding RNA PROX1‐AS1 and regulates gastric cancer progression and aggressiveness .…”

Section: Discussionsupporting

confidence: 88%

“…In summary, the results of the present study indicated that miR-647 may function as a tumor suppressive role in glioma, which is consistent with its role in non-small cell lung cancer or gastric cancer. 7,8 A recent study reported that miR-647 expression can be regulated by long non-coding RNA PROX1-AS1 and regulates gastric cancer progression and aggressiveness. 13 A limitation of the present study is that we did not explore the upstream regulator of miR-647 in glioma and hence further investigations are needed.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, miR-647 overexpression was found to inhibit gastric cancer progression by arresting the cell cycle and inducing cell apoptosis both in vitro and in vivo. 8 However, a recent study conducted by Zhang et al 9 showed that miR-647 was inversely correlated with tumor protein P73 expression in gastric cancer.…”

Section: Introductionmentioning

confidence: 97%

“…In addition, miR-647 was shown to inhibit non-small cell lung cancer cell proliferation by arresting cell cycle via regulation of the TRAF2 and NF-κB signaling pathway. 7 In gastric cancer, Cao et al 8 revealed that a reduced miR-647 level was correlated with a large tumor size and worsened metastasis. Moreover, miR-647 overexpression was found to inhibit gastric cancer progression by arresting the cell cycle and inducing cell apoptosis both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

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miR‐647 inhibits glioma cell proliferation, colony formation and invasion by regulating HOXA9

Qin

Tian

Chen

et al. 2020

The Journal of Gene Medicine

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Background MicroRNA‐647 (miR‐647) has been reported to regulate tumor development, although its role in glioma remains unclear. Methods miR‐647 expression in glioma cells and normal cells was measured using a quantitative real‐time polymerase chain reaction. The effects of miR‐647 expression on glioma cell proliferation, cell apoptosis, colony formation and cell invasion were measured using a cell counting kit‐8 assay, flow cytometry, a colony formation assay and a transwell invasion assay. Luciferase activity reporter and western blot assays were conducted to explore whether homeobox A9 (HOXA9) was a direct target of miR‐647. Results We found that miR‐647 expression was downregulated in glioma cell lines compared to the normal cell line. Overexpression of miR‐647 inhibits glioma cell proliferation, colony formation and cell invasion, although it promotes apoptosis in vitro. HOXA9 was validated a direct target of miR‐647 and the overexpression of HOXA9 reversed the effects of miR‐647 on glioma cell behavior. Conclusions The identification of the miR‐647/HOXA9 axis will advance our understanding underlying glioma progression and provide novel therapeutic targets for glioma treatment.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

miR‐647 inhibits glioma cell proliferation, colony formation and invasion by regulating HOXA9

Qin

Tian

Chen

et al. 2020

The Journal of Gene Medicine

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“…79 MiR-647 has two expression patterns in GC tissues and cell line, so that downregulation of this miRNA in GC tissues significantly associated with reduced metastasis and tumor size 80 also, overexpression of miR-647 inhibited cell proliferation, promoted cell cycle arrest and apoptosis of GC cell lines, and appreciably suppressed tumor growth in vivo. 80 Countless miRNAs also act as biological prognostic biomarkers for consequence of GC patients. Li et al reported that the high-risk miRNAs (miRNA-10b, miRNA-21, miRNA-223, and miRNA-338) signature was independent predictors of poor prognosis in GC.…”

Section: Mirnas As Diagnostic and Prognostic Biomarkers In Gcmentioning

confidence: 99%

Dysregulation of miRNAs as a signature for diagnosis and prognosis of gastric cancer and their involvement in the mechanism underlying gastric carcinogenesis and progression

Ahadi

2020

IUBMB Life

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Gastric cancer (GC) is the second main cause of cancer‐related mortality worldwide. The poor prognosis and survival of GC are due to diagnosis in an advanced, noncurable stage and with a restricted response to chemotherapy. GC is usually monitored in an advanced stage; therefore, the poor prognosis and lower level of survival rate with a restricted response to chemotherapy can be detected. Valuable and sensible biomarkers are urgently needed to display screen patients with a high risk of GC that can complement endoscopic diagnosis. Such biomarkers will enable the efficient prediction of the therapeutic response and prognosis of GC patients and prefer the establishment of an advantageous treatment method for each and every patient. Noninvasive diagnostic biomarkers may additionally make a contribution to the early identification of GC and enhance medical management. MicroRNAs (miRNAs) are a group of small noncoding RNAs that have displayed a strong association with GC. Accumulating evidence indicates that miRNAs are potential biomarkers with more than one diagnostic function for GC. Actually, miRNAs regulate cell proliferation, apoptosis, migration, invasion, and metastasis via many biological pathways through the repression of target mRNAs. The current review is accordingly to spotlight the multifaceted roles of miRNAs in GC, which would provide indications for future research. Therefore, we review right here the aberrant expression of miRNAs and underlying mechanisms, consequent effects due to miRNAs dysregulation, and accountable target genes in GC. Besides, potential clinical applications are also highlighted.

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CircFNTA promotes tumorigenesis and progression of gastric cancer via miR‐604/miR‐647/SCN8A axis

Yu,

Shen,

Wang

et al. 2023

J Biochem & Molecular Tox

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Gastric cancer (GC) is a major contributor to cancer‐related deaths and is characterized by high heterogeneity in epidemiology and histopathology worldwide. Increasing evidence indicates that circular RNAs (circRNAs) play multifaceted roles in cellular processes in human cancers. Here, we demonstrated that circFNTA high expression increases the proliferation, metastasis, and epithelial–mesenchymal transition process and tumorigenicity of GC cells. First, we found that circFNTA was upregulated in GC cells and tissues, and the high circFNTA levels were positively associated with the poor prognosis in GC patients. Using luciferase reporter and RNA‐pull down assays, we elucidated that circFNTA sponged two microRNAs, miR‐604 and miR‐647. In addition, the proliferation and metastatic ability of GC cell reduction caused by silencing circFNTA was hindered by inhibitors of miR‐604 and miR‐647. Moreover, SCN8A was predicted by miRDB as a common target gene of miR‐604 and miR‐647, which was then verified by the luciferase reporter assay. Knockdown of circFNTA causes messenger RNA and protein levels in SCN8A to be downregulated in GC cells. However, this effect was overturned by cotransfection miR‐604 and miR‐647. Also, we identified that SCN8A was downregulated in GC tissues, which was positively correlated with circFNTA expression. In rescue experiments, the attenuated cell proliferation and metastatic ability caused by circFNTA knockdown was reversed by miR‐604 and miR‐647 inhibitors and SCN8A overexpression. Collectively, our findings suggest an oncogenic role of circFNTA in GC progression and elucidate that circFNTA exerts its function by modulating the miR‐604/miR‐647/SCN8A axis.

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Role of miR-647 in human gastric cancer suppression

Cited by 35 publications

References 40 publications

miR‐647 inhibits glioma cell proliferation, colony formation and invasion by regulating HOXA9

miR‐647 inhibits glioma cell proliferation, colony formation and invasion by regulating HOXA9

Dysregulation of miRNAs as a signature for diagnosis and prognosis of gastric cancer and their involvement in the mechanism underlying gastric carcinogenesis and progression

CircFNTA promotes tumorigenesis and progression of gastric cancer via miR‐604/miR‐647/SCN8A axis

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