miR‐647 inhibits glioma cell proliferation, colony formation and invasion by regulating HOXA9

Abstract

Background MicroRNA‐647 (miR‐647) has been reported to regulate tumor development, although its role in glioma remains unclear. Methods miR‐647 expression in glioma cells and normal cells was measured using a quantitative real‐time polymerase chain reaction. The effects of miR‐647 expression on glioma cell proliferation, cell apoptosis, colony formation and cell invasion were measured using a cell counting kit‐8 assay, flow cytometry, a colony formation assay and a transwell invasion assay. Luciferase activity reporter and western blot assays were conducted to explore whether homeobox A9 (HOXA9) was a direct target of miR‐647. Results We found that miR‐647 expression was downregulated in glioma cell lines compared to the normal cell line. Overexpression of miR‐647 inhibits glioma cell proliferation, colony formation and cell invasion, although it promotes apoptosis in vitro. HOXA9 was validated a direct target of miR‐647 and the overexpression of HOXA9 reversed the effects of miR‐647 on glioma cell behavior. Conclusions The identification of the miR‐647/HOXA9 axis will advance our understanding underlying glioma progression and provide novel therapeutic targets for glioma treatment.

Circular RNA CircNOLC1, Upregulated by NF-KappaB, Promotes the Progression of Prostate Cancer via miR-647/PAQR4 Axis

“…Due to previous reports, miR-647 could retard tumor progression via inhibiting downstream oncogenes (Ye et al, 2017;Qin et al, 2020). We subsequently discovered that overexpression of miR-647 promoted PCa cells proliferation and migration, which indicated that miR-647 act as a tumor inhibitor role in PCa (Figures 3G,H).…”

“…Thus, we screened and validated the interaction between circNOLC1 and miRNAs by circRNA pulldown and dual-luciferase reporter assay, results demonstrated that circNOLC1 function as a sponge of miR-647. Meanwhile, miR-647 was identified as a tumor inhibitor in glioma and gastric cancer (Ye et al, 2017;Qin et al, 2020). Whereas, its role in PCa remains unclear.…”

HOXA9 Expression is Associated with Advanced Tumour Stage and Prognosis in Nasopharyngeal Carcinoma

“…As mentioned above, recent studies demonstrate abnormal HOXA9 expression in human AML, 9 colorectal cancer, 10 malignant glioma, 11 lung cancer, 12 and other tumours. This protein plays an important role in tumour progression (including metastasis), and is closely associated with prognosis.…”

“…Each cluster contains between nine and 13 gene loci, which are regulated by highly conserved transcription factors; stringent control is essential given that these genes represent major regulators of embryonic development and cell differentiation. 6 , 7 A member of the HOX gene family, HOXA9 has recently been demonstrated to exhibit increased expression in a variety of human malignant tumours (including acute myeloid leukaemia (AML), 8 colorectal cancer (CRC), 9 malignant glioma, 10 lung cancer, 11 and is closely associated with tumour progression, invasion, metastasis, and prognosis.…”

<p>ZFAS1 Exerts an Oncogenic Role via Suppressing miR-647 in an m⁶A-Dependent Manner in Cervical Cancer</p>

“…Among the top 30 miRNAs, only miR-647 has been previously reported to show involvement in tumor progression. [10][11][12] To validate this, we conducted luciferase reporter assays and found that transfection of miR-647 mimics reduced the luciferase activity of the wild-type ZFAS1 reporter gene, but not the mutant ZFAS1 (ZFAS1-mut) ( Figure 4B and C). Moreover, we carried out a MS2-binding protein (MS2bp)-MS2-binding sequences (MS2bs)-based RIP (MS2-RIP) according to the previous studies.…”