Role of minimal panel immunostaining in accurate diagnosis of lung cancer using small biopsies

Rahman, Manar Ahmed Abdel; Nada, Nadia; Zalata, Khaled; Badrawy, Mohammad Khairy El; Salkh, Iman Mohammed El; Hamid, Arif

doi:10.1016/j.ejcdt.2016.10.011

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…The most common type of cancer in our study was NSCLC, which accounted for 50 cases (87.7%), while SCC accounted for seven cases (12.2%). NSCC was also identified as the most common lung tumour in studies [ 28 ]. There were 33 cases (66%) of ADC, 13 cases (26%) of SqCC, and four cases (8%) of PDC among the 50 cases of NSCLC.…”

Section: Discussionmentioning

confidence: 99%

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Accuracy of Classifying Lung Carcinoma Using Immunohistochemical Markers on Limited Biopsy Material: A Two-Center Study

Hassan¹,

Alahmadi²,

Waqas³

et al. 2022

Cureus

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IntroductionAccurate classification of lung cancer into primary and metastatic carcinomas is critical for treatment approaches. Immunohistochemistry (IHC) has always been pivotal in unveiling the diverse cell differentiation lineages present in lung cancer by using specific biomarkers such as TTF1 and p63/p40, which closely reflect the relationship between genotype and phenotype.. MethodsA retrospective cross-sectional study was conducted to evaluate 57 Tru-Cut biopsies over two years, from 2020-2022. Tumour morphology was evaluated, and IHC for TTF-1, Napsin A, CK-7, P-63, P-40, and CD-56 was performed in two steps. ResultsOf the lung cancer cases, 58.5% were adenocarcinoma (ADC), 24.5% were squamous cell carcinoma (SCC), 9.4% were small cell carcinoma, and 7.5% were poorly differentiated carcinoma. TTF1 stain had sensitivity and specificity of 78.9% and 50% in 33 cases of ADC, respectively, while CK7 and Napsin A had 100% sensitivity. P63 stain had 77% sensitivity and 50% specificity in 15 cases of SCC, while P-40 had 100% sensitivity. The CD56 stain was 100% sensitive in five cases of small cell carcinoma. ConclusionIHC staining on small lung biopsies allows accurate sub-classification of poorly differentiated lung cancers; however, there is still significant variability. Surgical resection specimens can be further classified due to architectural features that biopsies lack. Morphological findings would be beneficial in the development of an algorithm for sub-classifying lung carcinoma using a variety of markers.

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Section: Discussionmentioning

confidence: 99%

“…There were 33 cases (66%) of ADC, 13 cases (26%) of SqCC, and four cases (8%) of PDC among the 50 cases of NSCLC. ADC was also identified as the most common NSCLC [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Accuracy of Classifying Lung Carcinoma Using Immunohistochemical Markers on Limited Biopsy Material: A Two-Center Study

Hassan¹,

Alahmadi²,

Waqas³

et al. 2022

Cureus

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Protein expression study of IMP3 in pancreatic and lung cancers: pathological and clinical correlations

et al. 2021

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An immunohistochemical expression of CK5/6, CK7, and CK20 on cell blocks in metastatic cervical lymphadenopathy

Singh¹,

Kaur²,

Devgan³

et al. 2022

Int J App Basic Med Res

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Background The neck is a common site of both primary and secondary malignancies. Many tumors from the head and neck (oral cavity, larynx, and pharynx), lung, and gastrointestinal tract metastasize to cervical lymph nodes. At most times, tumors are diagnosed by morphology, sometimes it is difficult to diagnose an unknown primary presenting as metastatic lymphadenopathy solely on the basis of morphology. Specific histological cell types can be confirmed by the use of immunohistochemistry. Aim The present study evaluated the utility of cell block over fine-needle aspiration cytology (FNAC) and immunohistochemical expression of CK5/6, CK7, and CK20 in metastatic cervical lymphadenopathy. Methods: This prospective study design was used on a total of 50 cases. FNAC smears and cell blocks were made in all the cases. All the cell blocks were compared with FNAC findings and further subjected to immunohistochemical (IHC) analysis. The necessary statistical analysis was done. Results and Conclusion Our study showed that the combined use of the cell block technique and FNAC was more useful and sensitive in diagnosing the metastatic cervical lymph nodes and the accuracy can be further improved by the use of IHC on the cell blocks. The combined use of CK5/6, CK7, and CK20 in metastatic cervical lymphadenopathy is helpful in diagnosing squamous cell carcinoma and adenocarcinoma with known/unknown primary sites.

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Role of minimal panel immunostaining in accurate diagnosis of lung cancer using small biopsies

Cited by 3 publications

References 17 publications

Accuracy of Classifying Lung Carcinoma Using Immunohistochemical Markers on Limited Biopsy Material: A Two-Center Study

Accuracy of Classifying Lung Carcinoma Using Immunohistochemical Markers on Limited Biopsy Material: A Two-Center Study

Protein expression study of IMP3 in pancreatic and lung cancers: pathological and clinical correlations

An immunohistochemical expression of CK5/6, CK7, and CK20 on cell blocks in metastatic cervical lymphadenopathy

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