Role of microRNAs and their Involvement in Preeclampsia

Selvaraj, Srivathchava; Lakshmanan, Ganesh; Kalimuthu, Kohila; Sekar, Durairaj

doi:10.2217/epi-2020-0281

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…At the same time, the selection of microRNAs as diagnostic markers for moyamoya disease is based on the following three points. First, many literature reports confirm that microRNAs play an important role in the progression of many diseases, including tumors, eclampsia, neurodegeneration, and heart failure, as well as the diagnosis and treatment of various diseases [ [15] , [16] , [17] , [18] ]. Second, the stability of microRNAs is significantly higher than that of mRNA.…”

Section: Discussionmentioning

confidence: 99%

Exosomal MicroRNAs: Biomarkers of moyamoya disease and involvement in vascular cytoskeleton reconstruction

Wang,

Zhang,

Jin

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Exosomal MicroRNAs: Biomarkers of moyamoya disease and involvement in vascular cytoskeleton reconstruction

Wang,

Zhang,

Jin

et al. 2024

Heliyon

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“…Further studies are required to investigate the functions of other miRs. Third, PE is a complex pathological process involving several miRs and target genes ( 52 ). The present study focused exclusively on the miR-424 and APC/Wnt/β-catenin signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

miR‑424‑5p is downregulated in the placentas of patients with preeclampsia and affects trophoblast migration and invasion

Zhu

Chen

2023

Exp Ther Med

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Insufficient invasion of trophoblast cells has been reported to be closely associated with the pathogenesis of preeclampsia (PE). MicroRNAs (miRs) have essential roles in the trophoblasts invasion via targeting specific genes with diverse functions. However, the underlying mechanism remains largely unclear and requires further investigation. The present study aimed to identify and evaluate the potential functions of miRs in trophoblasts invasion and to reveal the underlying mechanisms. In the present study, differentially expressed miRs that were screened based on previously published microarray data (GSE96985) and a significantly downregulated miR-424-5p (miR-424) was chosen for further investigation. Subsequently, reverse transcription-quantitative PCR, CCK-8, apoptosis, wound healing and Transwell assays were performed to determine the cell viability, apoptotic rate, cell migration and invasion of trophoblast cells. The results showed that miR-424 was decreased in placenta specimens from patients with PE. Upregulation of miR-424 promoted cell viability, suppressed cell apoptosis and improved the invasion and migration of trophoblasts, whereas inhibition of miR-424 had opposite results. Adenomatous polyposis coli (APC), a key mediator of Wnt/β-catenin signaling pathway, was identified as a functional target of miR-424 and an inverse relationship was observed between APC and miR-424 in placenta specimens. Further investigations revealed that APC overexpression efficiently suppressed the effect of miR-424 in trophoblast cells. In addition, the miR-424-mediated effects on trophoblast cells were dependent on the promotion of Wnt/β-catenin signaling pathway. The present findings revealed that miR-424 regulates the trophoblast cell invasion by regulating Wnt/β-catenin pathway through targeting APC, indicating miR-424 as a potential candidate for the treatment of PE.

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“…Non-coding RNAs called miRNAs are 16-27 nucleotides in length and play a crucial role in gene expression predominantly by post-transcriptional silencing of target genes (Selvaraj et al, 2020;Li et al, 2014). MicroRNA biogenesis is classified into two pathways namely, canonical and non-canonical pathways.…”

Section: Overview Of Micrornasmentioning

confidence: 99%

Role of microRNAs and their Involvement in Preeclampsia

Cited by 5 publications

References 9 publications

Exosomal MicroRNAs: Biomarkers of moyamoya disease and involvement in vascular cytoskeleton reconstruction

Exosomal MicroRNAs: Biomarkers of moyamoya disease and involvement in vascular cytoskeleton reconstruction

miR‑424‑5p is downregulated in the placentas of patients with preeclampsia and affects trophoblast migration and invasion

Therapeutic aspect of microRNA inhibition in various types of hypertension and hypertensive complications

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