Role of microRNAs and their target genes in salinity response in plants

Lotfi, Abolfazl; Pervaiz, Tariq; Jiu, Songtao; Faghihi, Faezeh; Jahanbakhshian, Zahra; Khorzoghi, Effat Ghadirzadeh; Fang, Jinggui; Seyedi, Seyed Mahdi

doi:10.1007/s10725-017-0277-0

Cited by 25 publications

(18 citation statements)

References 121 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results are also reported where the role of miR156 is well appreciated in the reduction of bio-recalcitrance and fermentable sugars yields(see table 1) [27,28,139,153]. The main endogenous natural cell wall hydrolyzing proteins e.g., laccases also synthesized in the high expressing miR156 Spartina alterniflora and switchgrass plants afflicted with water-stress [154]. Summarily, it is inferred that the recalcitrance of feedstocks can be reduced significantly by rewiring the miR156-SPL system via synthetic biology tools [155] in the bioenergy plants.…”

Section: The Role Of Mir156 In the Reduction Of Bio-recalcitrancesupporting

confidence: 80%

See 1 more Smart Citation

The miR156: A Master Regulator to Harmonize Complex Biofuel Traits in Lignocellulosic Biomass Crops

Singh¹

2020

Preprint

View full text Add to dashboard Cite

Currently, energy security and environmental degradation are the two biggest challenges before humanity that can be surmounted with the use of green and sustainable biofuels produced from lignocellulosic crops. In the future, to ensure adequate and cost-effective supply of biofuels, it requires a sufficient amount of amenable and quality lignocellulosic feedstocks. Therefore, agricultural yields of lignocellulosic biomass crops should be substantially increased by intense genetic maneuvering of key gene regulatory mechanisms and signaling pathways that control plant biomass yield. Recently, numerous miRNAs families are identified, characterized, and validated across the plant kingdom. Plant microRNAs (miRNAs) are 21 to 24 nucleotides long, non-coding small RNAs, act as regulators of their target genes via inducing modifications in transcription, translation, and epigenome. MiRNAs represent many hallmark characteristics like sequence-specific regulation, tissue, and species-specific expression, evolutionary conservation, and functional diversity. They coordinate well physiological and life cycle processes in plants under adverse environmental conditions. Hence, miRNAs offer accurate, precise, and efficient regulatory switches in the miRNA-targeted genetic networks. It is evident from the study of the miR156 family and its target SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes network that controls highly significant agronomic traits in crop plants. The miR156/SPL module acts as a master circuit that synchronizes many intricate complex biological functions such as growth and development, and metabolic processes by sensing internal and external environmental signals in plants. Therefore, miR156 can prove a potential target for miRNAs based plant biotechnology to harmonize complex biofuel traits and improve biomass yield in lignocellulosic biomass crops.

show abstract

Section: The Role Of Mir156 In the Reduction Of Bio-recalcitrancesupporting

confidence: 80%

“…The response of salinity on the miR156/SPL module was studied in a grass halophyte (Spartina alterniflora Loisel), a model plant that shows a high level of salinity resistance [77,78]. In the same plant, the miR156 was the most abundant among the most conserved miRNA species along with miR397 that regulate abiotic stress [154,199].…”

Section: Salinity Stressmentioning

confidence: 99%

The miR156: A Master Regulator to Harmonize Complex Biofuel Traits in Lignocellulosic Biomass Crops

Singh¹

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…RNA mediated gene silencing plays pivotal roles in orchestrating the expression, stability and inheritance of plant genomes, as well as in defenses against biotic and abiotic stressors. Numerous investigations demonstrated that RNAi can function as a master regulator of the regulatory networks associated with the expression of stress related genes (Sunkar et al 2012;Lotfi et al 2017). Selected salt responsive plant miRNAs, their targets and the traits regulated by the respective miRNAs are presented in Figure 2.…”

Section: Rna Interference (Rnai) Technologies For the Production Ofmentioning

confidence: 99%

Engineering salinity tolerance in plants: progress and prospects

Wani

Kumar

Khare

et al. 2020

Planta

149

View full text Add to dashboard Cite

Soil salinity exerts significant constraints on global crop production, posing a serious challenge for plant breeders and biotechnologists. The classical transgenic approach for enhancing salinity tolerance in plants revolves by boosting endogenous defence mechanisms, often via a single gene 2 approach, and usually involves the enhanced synthesis of compatible osmolytes, antioxidants, polyamines, maintenance of hormone homeostasis, modification of transporters and/or regulatory proteins, including transcription factors (TFs) and alternative splicing events. Occasionally, genetic manipulation of regulatory proteins or phytohormone levels confers salinity-tolerance, but all these may cause undesired reduction in plant growth and/or yields. In this review, we present and evaluate novel and cutting-edge approaches for engineering salt tolerance in crop plants. First, we cover recent findings regarding the importance of regulatory proteins and transporters, and how they can be used to enhance salt tolerance in crop plants. We also evaluate the importance of halobiomes as a reservoir of genes that can be used for engineering salt-tolerance in glycophytic crops. Additionally, the role of microRNAs as critical post-transcriptional regulators in plant adaptive responses to salt stress are reviewed and their use for engineering salt-tolerant crop plants is critically assessed. The potentials of alternative splicing mechanisms and targeted gene-editing technologies in understanding plant salt-stress responses and developing salt-tolerant crop plants is also discussed.

show abstract

“…MiRNAs have been found to play their biological roles by regulating expression of target genes [14]. Forkhead box protein O (FOXO) is a broad-ranging transcription factor [15].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation

Guo

Wei

et al. 2020

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Background: MicroRNAs (miRNAs) are critical regulators in osteogenesis and cartilage formation. This study was designed to investigate whether miR-532-5p plays a role in the regulation of osteoporosis. Methods: Osteoporotic fractures (OP group, n = 10) or osteoarthritis without osteoporosis (control group, n = 10) were selected as subjects in this study. Quantitative analysis of gene expression was performed by RT-PCR. Western blot was used to determine the expression levels of protein forkhead O1 (FOXO1). Bioinformatics analyses and luciferase reporter assay were used to verify the downstream target of miR-532-5p. Results: Compared with the non-osteoporotic controls, miR-532-5p was upregulated in osteoporotic samples, and expression of miR-532-5p was downregulated in the osteogenic C2C12 cell model. Overexpression of miR-532-5p resulted in decreased expression levels of key osteoblast markers, including alkaline phosphatase (ALP), osteocalcin (OC), and collagen type I alpha 1 (COL1A1). The inhibitory results of miR-532-5p were reversed. MiR-532-5p contained a putative FOXO1 binding site. Moreover, miR-532-5p inhibited the expression of FOXO1, and overexpression of FOXO1 inhibited the effect of miR-532-5p on osteoblast markers. Conclusions: MiR-532-5p can provide references to osteoporosis by regulating the expression of FOXO1 and osteoblast differentiation. MiR-532-5p might serve as a therapeutic target for osteoporosis.

show abstract

Role of microRNAs and their target genes in salinity response in plants

Cited by 25 publications

References 121 publications

The miR156: A Master Regulator to Harmonize Complex Biofuel Traits in Lignocellulosic Biomass Crops

The miR156: A Master Regulator to Harmonize Complex Biofuel Traits in Lignocellulosic Biomass Crops

Engineering salinity tolerance in plants: progress and prospects

MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation

Contact Info

Product

Resources

About