MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation

Abstract

Background: MicroRNAs (miRNAs) are critical regulators in osteogenesis and cartilage formation. This study was designed to investigate whether miR-532-5p plays a role in the regulation of osteoporosis. Methods: Osteoporotic fractures (OP group, n = 10) or osteoarthritis without osteoporosis (control group, n = 10) were selected as subjects in this study. Quantitative analysis of gene expression was performed by RT-PCR. Western blot was used to determine the expression levels of protein forkhead O1 (FOXO1). Bioinformatics analyses and luciferase reporter assay were used to verify the downstream target of miR-532-5p. Results: Compared with the non-osteoporotic controls, miR-532-5p was upregulated in osteoporotic samples, and expression of miR-532-5p was downregulated in the osteogenic C2C12 cell model. Overexpression of miR-532-5p resulted in decreased expression levels of key osteoblast markers, including alkaline phosphatase (ALP), osteocalcin (OC), and collagen type I alpha 1 (COL1A1). The inhibitory results of miR-532-5p were reversed. MiR-532-5p contained a putative FOXO1 binding site. Moreover, miR-532-5p inhibited the expression of FOXO1, and overexpression of FOXO1 inhibited the effect of miR-532-5p on osteoblast markers. Conclusions: MiR-532-5p can provide references to osteoporosis by regulating the expression of FOXO1 and osteoblast differentiation. MiR-532-5p might serve as a therapeutic target for osteoporosis.

Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway

“…Furthermore, with an extension of immobilization, osteoblast activity continued to decrease, as reflected by the reduction of OCN expression, suggesting that the balance between bone resorption of osteoclasts and bone formation of osteoblasts was broken in immobilization-induced KOA rabbits. A previous study has indicated that low expression of OCN is observed in KOA subchondral bone with severe bone loss, resulting in a reduction of bone formation [52], while increasing the content of OCN can effectively promote osteoblast activity in damaged bone [53]. Our study demonstrated that acupotomy and electroacupuncture effectively promoted bone formation by activating OCN expression.…”

Visceral Adipose Tissue of Prediabetic and Diabetic Females Shares a Set of Similarly Upregulated microRNAs Functionally Annotated to Inflammation, Oxidative Stress and Insulin Signaling

“…Some data also links expression changes of selected miRNAs with insulin sensitivity. For instance, miR-532-5p was associated with IR in females via regression analysis [ 44 ] as well as confirmed to target FoxO1 [ 45 ]. Further, let-7 family is a well-known regulator of glucose homeostasis and IR [ 46 ].…”

Biomarkers of Frailty: miRNAs as Common Signatures of Impairment in Cognitive and Physical Domains

“…By reviewing the literature on phenotypes related to physical frailty, we identified a total of 57 miRNAs associated with physical phenotypes, resumed in Table S1 [ 67 , 68 , 69 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 ]. An inspection of their characteristics revealed that they belong to three main groups: muscle-related miRNAs, inflamm-miRNAs and mitochondrial miRNAs (mitomiRNA).…”