Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway

Wang, Tong; Guo, Yan; Shi, Xiaowei; Gao, Yang; Zhang, Jiayi; Wang, Chunjiu; Yang, Xue; Shu, Qi; Chen, Xilin; Fu, Xiaoyi; Xie, Wen-Shan; Zhang, Yi; Li, Bin; Guo, Chang-Qing

doi:10.1155/2021/8168657

Cited by 5 publications

(8 citation statements)

References 57 publications

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“…31 Acupotomy could release the muscle and tendon around the knee joint of rabbits to restore the biomechanical characteristics of the joint based on the Jingjin theory of traditional Chinese medicine and the theory of biomechanics and anatomy in modern medicine. 32 This study applied the modified Videman method for KOA modeling, which may be more suitable to explore the related mechanism of acupotomy in the treatment of KOA.…”

Section: Discussionmentioning

confidence: 99%

Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits

Guo¹,

Xu²,

He³

et al. 2023

JPR

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Knee osteoarthritis (KOA) is a chronic inflammatory disease highly associated with intra-articular hypertension, hypoxia and angiogenesis of synovial tissue. Our previous studies showed that acupotomy could treat KOA in a variety of ways, including reducing cartilage deterioration and enhancing biomechanical qualities. However, the mechanism of hypoxia and angiogenesis induced by acupotomy in KOA synovium remains unclear. This study looked for the benign intervention of acupotomy in synovial pathology. Methods: The rabbits were divided into 3 groups, Normal group, KOA group, and KOA + Acupotomy (Apo) group, with 11 rabbits in each group. The KOA rabbit model was established by the modified Videman method with six weeks. The KOA + Apo group performed the intervention. The tendon insertion of vastus medialis, vastus lateralis, rectus femoris, biceps femoris, and anserine bursa were selected as treatment points in rabbits. Rabbits were treated once every 7 days for 3 weeks. We observed the intra-articular pressure and oxygen partial pressure (BOLD MRI). The synovial morphology was monitored by Hematoxylin-Eosin Staining (HE Staining). The expression of hypoxia-inducible transcription factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) was detected using Immunohistochemical (IHC), Western Blot and Enzyme-Linked Immunosorbent Assay (ELISA). Results: Acupotomy reduced intra-articular hypertension and improved the synovial oxygen situation, synovial inflammatory and angiogenesis. HIF-1α, VEGF, IL-1β and TNF-α expression were downregulated by acupotomy. Conclusion: Acupotomy may reduce inflammation and angiogenesis in KOA rabbit by reducing abnormally elevated intra-articular pressure and improving synovial oxygen environment. The above may provide a new theoretical foundation for acupotomy treatment of KOA.

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Section: Discussionmentioning

confidence: 99%

Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits

Guo¹,

Xu²,

He³

et al. 2023

JPR

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“…Knee osteoarthritis (KOA) induces a persistent abnormal load within the joint, and cartilage degeneration has always been the focus of KOA research [ 9 – 11 ]. The morphological observation by Safranin O staining showed that KOA causes the defect of cartilage surface and disorder of the chondrocyte arrangement, which is regarded as symptoms of cartilage degeneration [ 5 ]. Similar to the previous study, we also found that the surface of KOA cartilage was rough and the arrangement of chondrocytes was disordered ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, Wang et al (2019) reported that the application of acupotomy clearly alleviated the symptoms of KOA in rabbits by changing the arrangement of fibrous tissue [ 8 ]. Besides, the application of acupotomy in rabbits significant prevented both cartilage surface erosion and subchondral bone loss by histological observation [ 5 ]. In clinical treatment, Cho et al (2019) reported that the combined therapeutic effect of miniscalpel acupuncture and splint therapy on osteoarthritis of the hand seems to have positive results [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…As the common methods of traditional Chinese medicine (TCM) used for the treatment of KOA, acupotomy intervention (AI) was widely used to relieve cartilage tissue pain and restore the movement of the knee joint [ 4 ]. In rabbits, acupotomy could alleviate cartilage deterioration by regulating the OPG/RANKL signaling pathway to inhibit subchondral bone resorption [ 5 ]. Similarly, Huang et al (2020) reported that activating the PI3K/Akt signaling pathway to inhibit the chondrocyte apoptosis in KOA rabbits might be a possible mechanism of acupotomy in treating KOA [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits

Liu

Zeng

Lin

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Acupotomy intervention (AI) is an available treatment for knee osteoarthritis (KOA) in China, which is a common health problem over the world. However, the underlying mechanism of AI on the KOA treatment is still unknown. To further understand the mechanism of acupotomy in treating KOA, the morphological observation and TMT proteomic analyses were conducted in rabbits. By using X-ray and MRI, we found that the space of the knee joint was bigger in AI than in KOA. Moreover, the chondrocytes were neatly arranged in AI but disordered in KOA. With proteomic analyses in chondrocytes, 68 differently accumulated proteins (DAPs) were identified in AI vs. KOA and DAPs related to energy metabolism and the TCA cycle were suggested to play a central role in response to AI. Furthermore, AIFM1 was proposed to be an important regulator in controlling the energy production in mitochondrial. Besides, FN1, VIM, COL12A1, COL14A1, MYBPH, and DPYSL3 were suggested to play crucial roles in AI for the treatment of KOA. Our study was systematically elucidating the regulation mechanism of acupotomy intervention in the treatment of KOA.

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“…Acupotomy combines traditional Chinese medicine meridian theory and modern surgical principles [11]. Acupotomy could alleviate the pain and reinstate the function of the joint by reducing the tension of local soft tissue and releasing the surrounding tissue adhesions [12][13][14]. Meanwhile, several studies indicated that acupotomy can reduce the expression of local inflammatory factors [15,16].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness and safety of acupotomy for knee osteoarthritis: study protocol for a randomized controlled trial

Guo

et al. 2021

Trials

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Background Knee osteoarthritis (KOA) is one of the most common musculoskeletal disorders. Acupotomy may be effective for KOA, but the evidence is limited. This trial aims to determine the effectiveness and safety of acupotomy for KOA. Methods/design This is a parallel-group, assessor-blinded randomized controlled trial. Two hundred patients with KOA will be recruited and randomly assigned to two groups (group A or group D) in a 1:1 ratio. Patients in group A will receive acupotomy and topical diclofenac diethylamine for 4 weeks, while patients in group D will receive topical diclofenac diethylamine alone for 4 weeks. The primary outcome will be the response rate—the proportion of patients who achieve the minimal clinically important improvement in pain and function at week 4 compared with baseline. Secondary outcomes will include pain, function, quality of life, the use of rescue medicine (loxoprofen sodium), and adverse events at weeks 4, 8, and 24 after randomization. Besides, joint fluid and serum will be collected to assess the level of inflammatory cytokines, like TNF-α, IL-1β, and MMP-3. Discussion This study will contribute to a better understanding of the effectiveness and safety of acupotomy in combination with topical nonsteroidal anti-inflammatory drugs. If the hypothesis is confirmed, acupotomy may be recommended as adjunctive therapy for patients with KOA. Results of the study will be of great importance for the guidelines of clinical therapy. Trial registration Chinese Clinical Trial Registry ChiCTR2100043005 Registered on 4 February 2021.

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Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway

Cited by 5 publications

References 57 publications

Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits

Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits

Proteomic Analyses Reveals the Mechanism of Acupotomy Intervention on the Treatment of Knee Osteoarthritis in Rabbits

Effectiveness and safety of acupotomy for knee osteoarthritis: study protocol for a randomized controlled trial

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