Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2

Wu, Ning; Zhao, Xiangzhong; Liu, Ming; Liu, Haizhou; Yao, Weicheng; Zhang, Yuyan; Cao, Shousong; Lin, Xiukun

doi:10.1371/journal.pone.0016264

Cited by 110 publications

(115 citation statements)

References 38 publications

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“…miR-26b inhibits glioma tumor cell proliferation, survival, and migration by directly targeting EPHA2 (43). Further evidence of the tumor-suppressive nature of miR-26b include its ability to induce apoptosis via repression of SLC7A11 and the decreased expression of miR-26b in breast carcinoma patient samples (44).…”

Section: Glyceollins Alter the Mirnome Of Triple-negative Breast Carcmentioning

confidence: 99%

Glyceollins as novel targeted therapeutic for the treatment of triple-negative breast cancer

et al. 2011

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Abstract. The purpose of this study was to investigate the effects of glyceollins on the suppression of tumorigenesis in triple-negative breast carcinoma cell lines. We further explored the effects of glyceollins on microRNA and protein expression in MDA-MB-231 cells. Triple-negative (ER-, PgR-and Her2/ neu-) breast carcinoma cells were used to test the effects of glyceollins on tumorigenesis in vivo. Following this procedure, unbiased microarray analysis of microRNA expression was performed. Additionally, we examined the changes in the proteome induced by glyceollins in the MDA-MB-231 cells. Tumorigenesis studies revealed a modest suppression of MDA-MB-231 and MDA-MB-468 cell tumor growth in vivo. In response to glyceollins we observed a distinct change in microRNA expression profiles and proteomes of the triple-negative breast carcinoma cell line, MDA-MB-231. Our results demonstrated that the glyceollins, previously described as anti-estrogenic agents, also exert antitumor activity in triple-negative breast carcinoma cell systems. This activity correlates with the glyceollin alteration of microRNA and proteomic expression profiles.

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Section: Glyceollins Alter the Mirnome Of Triple-negative Breast Carcmentioning

confidence: 99%

Glyceollins as novel targeted therapeutic for the treatment of triple-negative breast cancer

et al. 2011

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“…Agresif özellik gösteren glioma hücrelerine miR26b transfeksiyonu yapıldığında glioma gelişiminde azalma belirlenmiş olup, bu hücreler için artmış ekspresyonun anti-tümör etkiye sahip olabileceği belirtilmiştir [43]. …”

Section: Genetik Varyasyonların Değerlendirilmesiunclassified

DNA Metiltransferaz-3b ve Metil Bağlayan Bölge Proteini-1’i Hedefleyen Mirna Gen Polimorfizmlerinin Akciğer Kanseri İle İlişkisinin Belirlenmesi

Özbayer¹,

Değirmenci²,

Üstüner³

et al. 2015

Osmangazi̇ Journal of Medicine

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ÖZET: Akciğer kanseri, akciğer dokusundaki hücrelerin kontrolsüz olarak çoğalması ile meydana gelir ve kanser kaynaklı ölümlerin en yaygın nedenidir. En önemli nedenleri uzun süreli sigara dumanı maruziyeti, genetik faktörler, radon gazı ve asbest maruziyetidir. miRNA'lar kendi nükleotid dizilerinin tamamlayıcısı olan hedef mRNA'lara bağlanıp translasyonel baskılama veya mRNA yıkımı ile transkripsiyon sonrası gen ekspresyonunun düzenlenmesini gerçekleştirirler. Çeşitli çalışmalarda malignant tümörlerin gelişimi ve ilerlemesi ile miRNA ekspresyon düzensizliklerinin ilişkili olduğu, miRNA'ların onkogen veya tümör süpresör (baskılayıcı) olarak hareket edebileceği belirtilmiştir. Ayrıca tümör baskılayıcı genlerin downregülasyonu ve CpG dinükleotidlerindeki promotör hipermetilasyonu tümörogenezis için önemli bir mekanizmadır. Bir diğer epigenetik düzenleme olan miRNA'ların, genlerin promotör bölgelerindeki anormal metilasyon ve histon modifikasyonları gibi epigenetik mekanizmaları düzenleyebileceği bildirilmiştir. Bu amaçla biz de güncel veri tabanları ile DNMT3b ve MBD1'i hedefleyen 2 miRNA genine ait iki SNP belirleyerek bu SNP'lerin akciğer kanseri ile ilişkisini araştırmayı amaçladık. Gereç ve Yöntem: Çalışmamıza 90 sağlıklı kontrol birey ve 90 akciğer kanseri hastası dahil edildi (onay no: 2012/45). EDTA'lı tüplere alınan kan örneklerinden DNA izole edildi ve bu DNA'lar Sequenom MassARRAY sistemi ile genotiplendirildi. Hastaların genetik varyasyon tipleri ve demografik özellikleri uygun istatistiksel yöntemler ile değerlendirildi. Bulgular: DNMT3b'yi hedefleyen miR339 geni rs72631831 varyantı tüm bireyler için atasal GG genotipine, MBD1'i hedefleyen miR15b geni rs146020563 varyantı için tüm bireyler atasal AA genotipine sahip olarak belirlendi. Sonuç: Araştırmamızda rs72631831 ve rs146020563 varyasyonları ile akciğer kanseri arasında ilişki bulunamamıştır ancak araştırmamız DNA metilasyon mekanizmasını hedefleyen miRNA gen varyantlarının da fonksiyonel karakterizasyonunun yapıldığı ilk çalışma olması nedeniyle önemlidir. ANAHTAR KELİMELER: Akciğer Kanseri, DNA metilasyonu, DNA Metiltransferaz-3b, Metil Bağlayan Protein-1, miRNA, SNP. SUMMARY: Aim: Lung cancer (LC) is a disease that develops by uncontrolled cell growth in tissues of the lung and the most common cause of cancer-related death. Most significant risk factors for LC are tobacco smoke, genetic factors, radon gas and asbestos.miRNAs bind target mRNAs which are complementary to their sequences and post-transcriptionally regulate gene expression through translational repression or mRNA degradation. Several observations link dysregulation of miRNA expression to the development and progression of tumors and miRNAs can act as oncogenes or tumor suppressors. The hypermethylation of CpG-dinucleotides in the promoter of genes and downregulation of tumor suppressors are important for tumorogenesis. It has been reported that being a type of epigenetic modifier, miRNAs, may regulate epigenetic mechanism including abnormal methylation of the promoter regions or his...

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“…Real-time RT-PCR analysis was performed as described previously [21]. The sequences of primers for miR-125b, let-7a and let-7c are listed in Supplementary Table 2, while the primers of miR-638 and miR-149 are purchased from Genepharma (Shanghai, China).…”

Section: Quantitative Real-time Reverse Transcriptation Pcr (Qrt-pcr)mentioning

confidence: 99%

“…At certain time points (12,24,36,48 and 72 h, respectively) after transfection, MTT assay was performed to analyze the cell proliferation as described previously [21]. To assess the effect of miR-125b on the CD133 positive GSCs cell cycle, cells were transfected with 125b-DP or NC-DP at the final concentration of 100 nM/ml.…”

Section: Cell Proliferation and Cell Cycle Analysismentioning

confidence: 99%

miR‐125b regulates the proliferation of glioblastoma stem cells by targeting E2F2

Xiao

Zhao

et al. 2012

FEBS Letters

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Edited by Tamas DalmayKeywords: microRNA Glioblastoma stem cell Brain tumor microRNA-125b E2F2 a b s t r a c t microRNAs (miRNAs) play important role in regulating cancer stem cell self-renewal and differentiation, but the expression prolife of miRNAs in glioma stem cells (GSCs) has not been addressed. Here, we found that CD133 positive GSCs possess a unique miRNAs profile compared to CD133 negative glioblastoma cells. miR-125b, as one of neuronal miRNAs, is the most significantly down-regulated miRNAs and overexpression of miR-125b inhibits the proliferation of CD133 positive GSCs and reduces the expression of ''stem'' marker. Furthermore, two binding sites for miR-125b are identified in the 3 0 UTR of E2F2 and overexpression of miR-125b in CD133 positive GSCs represses the endogenous level of E2F2 protein. This study demonstrated that miR-125b plays important roles in regulating the proliferation of GSCs by directly targeting E2F2.

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Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2

Cited by 110 publications

References 38 publications

Glyceollins as novel targeted therapeutic for the treatment of triple-negative breast cancer

Glyceollins as novel targeted therapeutic for the treatment of triple-negative breast cancer

DNA Metiltransferaz-3b ve Metil Bağlayan Bölge Proteini-1’i Hedefleyen Mirna Gen Polimorfizmlerinin Akciğer Kanseri İle İlişkisinin Belirlenmesi

miR‐125b regulates the proliferation of glioblastoma stem cells by targeting E2F2

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