miR‐125b regulates the proliferation of glioblastoma stem cells by targeting E2F2

Wu, Ning; Xiao, Lin; Zhao, Xiangzhong; Zhao, Jianmin; Wang, Jianpeng; Wang, Fengxia; Cao, Shousong; Lin, Xiukun

doi:10.1016/j.febslet.2012.08.023

Cited by 79 publications

(42 citation statements)

References 35 publications

(61 reference statements)

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“…In lens epithelial cell, miR-125b was confirmed to directly target p53 [42] which plays important roles in cell apoptosis. In glioblastoma stem cells, miR-125b-5p is capable of regulating the E2F2 expression and affects cellular proliferation [43]. In osteosarcoma cells, miR-125b suppresses the cellular proliferation and migration through down-regulation of STAT3 [39].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-125b-5p inhibits proliferation and promotes adipogenic differentiation in 3T3-L1 preadipocytes

Ouyang

Guo

et al. 2015

ABBS

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Previous evidence has indicated that the microRNA-125b (miR-125b) family plays important roles in the regulation of cancer cell growth, development, differentiation, and apoptosis. However, whether they contribute to the process of adipocyte differentiation remains unclear. In the present study, we revealed that the expression level of miR-125b-5p, a member of miR-125b family, was dramatically up-regulated during differentiation of 3T3-L1 preadipocyte into mature adipocyte. Supplement of miR-125b-5p into 3T3-L1 cells promoted adipogenic differentiation as evidenced by increased lipid droplets and mRNA levels of adipocyte-specific molecular markers, including peroxisome proliferators-activated receptor γ, CCAAT/enhancer-binding protein α, fatty acid-binding protein 4, and lipoprotein lipase, and by triglyceride accumulation. CCK-8 assay showed that miR-125b-5p supplementation significantly inhibited cell proliferation. Flow cytometry analysis showed that miR-125b-5p impaired G1/S phase transition as well as the mRNA and protein expression of G1/S-related genes, such as Cyclin D2, Cyclin D3, and CDK4. Nevertheless, it had no effect on apoptosis. Additionally, by target gene prediction, we demonstrated that smad4 may be a potential target of miR-125b-5p in mouse 3T3-L1 preadipocytes, accounting for some of miR-125b-5p's functions. Taken together, these data indicated that miR-125b-5p may serve as an important positive regulator in adipocyte differentiation, at least partially through down-regulating smad4.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-125b-5p inhibits proliferation and promotes adipogenic differentiation in 3T3-L1 preadipocytes

Ouyang

Guo

et al. 2015

ABBS

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“…A GSC line, CD133 + GSCs, was established from human glioblastoma tissues as described in our previous study [23]. Briefly, the cells were derived from glioblastoma tissues of consenting patients, and strictly followed the protocol approved by the Research Ethics Board at the Affiliated Hospital of Qingdao Medical University of China.…”

Section: Culture and Treatment Of Glioblastoma Stem Cells (Gscs)mentioning

confidence: 99%

“…The CD133 + GSCs neurosphere formation and differentiation assays were performed as described previously [23]. Briefly, CD133 + GSCs were seeded in 96-well plates at a density of 1000 cells per well and treated with DMSO (control) or cardamonin (Sigma-Aldrich, St. Louis, MO, USA) at the concentration of 20, 30, or 40 μM.…”

Section: Analysis Of Neurosphere Formation and Differentiationmentioning

confidence: 99%

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Cardamonin induces apoptosis by suppressing STAT3 signaling pathway in glioblastoma stem cells

Liu

Zhao

et al. 2015

Tumor Biol.

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Glioblastoma stem cells (GSCs) are the initiating cells in glioblastoma multiforme (GBM) and contribute to the resistance of GBM to chemotherapy and radiation. In the present study, we investigated the effects of cardamonin (3,4,2,4-tetrahydroxychalcone) on the self-renewal and apoptosis of GSCs, and if its action is associated with signal transducer and activator of transcription 3 (STAT3) pathway. CD133(+) GSCs, a kind of GSCs line, was established from human glioblastoma tissues. Cardamonin inhibited the proliferation and induced apoptosis in CD133+ GSCs. The proapoptotic effects of temozolomide (TMZ) were further enhanced by cardamonin in CD133+ GSCs and U87 cells in vitro. For in vivo study, injection of 5 × 10(5) cells of CD133+ GSCs subcutaneously (s.c.) into nude mice, 100 % of large tumors were developed within 8 weeks in all mice; in contrast, only one out of five mice developed a small tumor when 5 × 10(5) cells of CD133(-) GMBs cells were injected. Cardamonin also inhibited STAT3 activation by luciferase assay and suppressed the expression of the downstream genes of STAT3, such as Bcl-XL, Bcl-2, Mcl-1, survivin, and VEGF. Furthermore, cardamonin locked nuclear translocation and dimerization of STAT3 in CD133(+) GSCs. Docking analysis confirmed that cardamonin molecule was successfully docked into the active sites of STAT3 with a highly favorable binding energy of -10.78 kcal/mol. The study provides evidence that cardamonin is a novel inhibitor of STAT3 and has the potential to be developed as a new anticancer agent targeting GSCs. This study also reveals that targeting STAT3 signal pathway is an important strategy for the treatment of human GBM.

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“…25 We found its expression was downregulated in B cells from Em/miR-125b-Tg mice (supplemental Figure 5G). In addition, the expression of E2F2, a potential miR-125b targeting gene that is critical for cell cycle transition, 26 was also reduced in the B cells from Em/miR-125b-Tg mice. Downregulation of these genes might have an important role in B-cell survival and proliferation in the periphery of Em/miR-125b-Tg mice, especially when peripheral B cells encounter antigens.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic silencing of miR-125b is required for normal B-cell development

Sookram

et al. 2018

Blood

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miR‐125b regulates the proliferation of glioblastoma stem cells by targeting E2F2

Cited by 79 publications

References 35 publications

MicroRNA-125b-5p inhibits proliferation and promotes adipogenic differentiation in 3T3-L1 preadipocytes

MicroRNA-125b-5p inhibits proliferation and promotes adipogenic differentiation in 3T3-L1 preadipocytes

Cardamonin induces apoptosis by suppressing STAT3 signaling pathway in glioblastoma stem cells

Epigenetic silencing of miR-125b is required for normal B-cell development

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