Role of microRNA‑129 in cancer and non‑cancerous diseases (Review)

Deng, Bingpeng; Tang, Xuan; Wang, Yong

doi:10.3892/etm.2021.10350

Cited by 13 publications

(6 citation statements)

References 143 publications

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“…The functional relevance of miR-129-5p downregulation in FTD was investigated in vitro using mixed neuron-glia cultures and astrocyte cultures. Previous studies have shown that miR-129-5p plays a role in modulating in ammation in various contexts [20,36,37]. Consistent with these ndings, our study revealed that miR-129-5p knockdown led to increased neuroin ammation, reduced synaptic plasticity, and increased synaptic pruning.…”

Section: Discussionsupporting

confidence: 92%

Inhibition of MicroRNA-129-5p Promotes Neuroinflammation and Cognitive Impairment

Kaurani,

Zhou,

Pradhan

et al. 2024

Preprint

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Frontotemporal dementia (FTD) is a neurodegenerative disorder that causes the frontal and temporal lobes of the brain to deteriorate over time. This leads to changes in behavior, language problems, and a loss of mental abilities. Dysregulation of microRNAs (miRNAs) has been linked to several neurodegenerative diseases, including FTD, which suggests that they could be used as both biomarkers and therapeutic targets. We looked at smallRNA sequencing data from the frontal and temporal brain tissue of FTD patients with mutations in the MAPT, GRN, or C9orf72 genes, as well as brain tissue from people without dementia. The objective of this study was to find out what role miR-129-5p plays in FTD. We discovered that miR-129-5p was abundant in neurons, and knocking it out in a neuron-glia mixed culture increased neuroinflammation and was linked to astrocyte activation. When astrocytes without miR-129-5p were cultured with primary neurons, it had a negative effect on synaptic plasticity. Inhibiting miR-129-5p in mice resulted in hippocampus-dependent learning memory impairment and cognitive dysfunction. This study emphasizes the role of miR-129-5p in regulating neuronal activity and its potential as a therapeutic target for FTD. The results show that miR-129-5p plays a crucial role in controlling neuroinflammation and synaptic plasticity in FTD. The research sheds light on the molecular mechanisms underlying FTD and reveals potential therapeutic targets for this devastating disease.

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Section: Discussionsupporting

confidence: 92%

Inhibition of MicroRNA-129-5p Promotes Neuroinflammation and Cognitive Impairment

Kaurani,

Zhou,

Pradhan

et al. 2024

Preprint

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“…KSRP plays a crucial role in processing of a subset of miRNAs, particularly those containing a GC-rich stemloop structure in their immature precursor transcript [36]. Among those are miR-155 [37], let-7a [38] and miR-129 [39], which exert important functions in the regulation of immune processes. Ruggiero and colleagues demonstrated miRNA-mediated degradation of IL-1β and CCL5 mRNA in a mouse macrophage cell line [33].…”

Section: Discussionmentioning

confidence: 99%

The mRNA-Binding Protein KSRP Limits the Inflammatory Response of Macrophages

Bolduan,

Palzer,

Hieber

et al. 2024

IJMS

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KH-type splicing regulatory protein (KSRP) is a single-stranded nucleic acid-binding protein with multiple functions. It is known to bind AU-rich motifs within the 3′-untranslated region of mRNA species, which in many cases encode dynamically regulated proteins like cytokines. In the present study, we investigated the role of KSRP for the immunophenotype of macrophages using bone marrow-derived macrophages (BMDM) from wild-type (WT) and KSRP−/− mice. RNA sequencing revealed that KSRP−/− BMDM displayed significantly higher mRNA expression levels of genes involved in inflammatory and immune responses, particularly type I interferon responses, following LPS stimulation. In line, time kinetics studies revealed increased levels of interferon-γ (IFN-γ), interleukin (IL)-1β and IL-6 mRNA in KSRP−/− macrophages after 6 h subsequent to LPS stimulation as compared to WT cultures. At the protein level, KSRP−/− BMDM displayed higher levels of these cytokines after overnight stimulation. Matching results were observed for primary peritoneal macrophages of KSRP−/− mice. These showed higher IL-6, tumor necrosis factor-α (TNF-α), C-X-C motif chemokine 1 (CXCL1) and CC-chemokine ligand 5 (CCL5) protein levels in response to LPS stimulation than the WT controls. As macrophages play a key role in sepsis, the in vivo relevance of KSRP deficiency for cytokine/chemokine production was analyzed in an acute inflammation model. In agreement with our in vitro findings, KSRP-deficient animals showed higher cytokine production upon LPS administration in comparison to WT mice. Taken together, these findings demonstrate that KSRP constitutes an important negative regulator of cytokine expression in macrophages.

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“…Small, noncoding RNAs impede translation, lowering target gene expression. In this study, we investigated the effects of miR-129 on gene expression [50]. The study found that BC patients had less miR-129 and miR-145 expression than those in controls.…”

Section: Discussionmentioning

confidence: 99%

Relapse and Survival in Bladder Cancer Patients Undergoing microRNA-129 and microRNA-145 Assays

ELfieky,

Abd El Rahman,

Fayed

et al. 2024

Asian Pac J Cancer Prev

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Objective: The lack of indicators to measure tumor’s invasive biological behavior is an important issue. The aim of this study was to examine the effect of miRNAs 129 and 145 on tumor progression as well as patient survival. Method: Seventy five breast cancer (BC) patients and 75 controls were included in this research. Two miRNA expressions were estimated using real-time PCR. Biomarkers for BC detection was tested using ROC curves and AUC. Result: miR-129 and miR-145 expressions were significant. miR-129 and miR-145 classifiers (AUC = 0.943 and 0.748, respectively) help diagnose BC. Unlike miR-145 , miR-129 did not affect the Kaplan–Meier survival curve analysis for progression-free survival at the end of the trial. The development of transitional cell carcinoma disease was found to have a strong correlation with miR-145 in both univariate and multivariate Cox regression analyses. Additionally, infiltrating + invasive urothelial carcinoma was also found to be correlated with miR-145. Conversely, elevated miR-129 expression in BC patients did not lead to an increase in cancer-specific recurrence or mortality, as observed in both univariate and multivariate Cox regression studies. Conclusion: The miRNA signature can help detect survival-associated miRNAs and develop BC miRNA therapeutics.

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Role of microRNA‑129 in cancer and non‑cancerous diseases (Review)

Cited by 13 publications

References 143 publications

Inhibition of MicroRNA-129-5p Promotes Neuroinflammation and Cognitive Impairment

Inhibition of MicroRNA-129-5p Promotes Neuroinflammation and Cognitive Impairment

The mRNA-Binding Protein KSRP Limits the Inflammatory Response of Macrophages

Relapse and Survival in Bladder Cancer Patients Undergoing microRNA-129 and microRNA-145 Assays

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