Role of MicroRNA 126 in screening, diagnosis, and prognosis of diabetic patients in Egypt

Rezk, Noha A.; Sabbah, Norhan A.; Saad, Mohamed S.S.

doi:10.1002/iub.1502

Cited by 57 publications

(51 citation statements)

References 25 publications

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“…The finding of PDR‐specific miRNAs in our study was consistent with other studies (as shown in Table ). Recently, miR‐150‐5p was found to be strongly down‐regulated in the extracellular vesicles of blood from DR subjects using miRNA array detection and suppression of the pathological neovascularization in mice using oxygen‐induced proliferative retinopathy .…”

Section: Discussionsupporting

confidence: 93%

“…25 In our previous study, we also used NGS to explore the miRNA profile in the AH of glaucoma patients, and we found glaucoma specific miRNAs profile and related to glaucoma progression. 19 The finding of PDR-specific miRNAs in our study was consistent with other studies [26][27][28] (as shown in Table 3). Recently, miR-150-5p was found to be strongly downregulated in the extracellular vesicles of blood from DR subjects using miRNA array detection 13 and suppression of the pathological neovascularization in mice using oxygeninduced proliferative retinopathy.…”

Section: Discussionsupporting

confidence: 92%

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Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Chen

Yuan

Liu

et al. 2019

Clinical Exper Ophthalmology

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Background The microRNAs (miRNA) have been found to play an important role in the pathogenesis of diabetic retinopathy. We try to explore the miRNA and piwi‐interacting RNA (piRNA) profile in the aqueous humour of proliferative diabetic retinopathy (PDR) using next‐generation sequencing (NGS). Methods Aqueous humour samples were collected from nine PDR eyes and nine cataract control eyes, and NGS was performed. Quantitative polymerase chain reaction (qPCR) was used to validate the sequencing results. An oxygen‐induced retinopathy (OIR) model was used to validate the angiogenesis related miRNA. Results In total, 484 miRNAs were differently expressed between the PDR eyes and cataract control eyes, including 210 mature miRNAs and 274 novel miRNAs. Furthermore, eight miRNAs and 30 piRNAs were identified as the most differently expressed between the two groups (P > .85). This differential expression of miRNA was predicted to regulate Rho protein signal transduction, neurotransmitter uptake and histone lysine methylation. Relative expression patterns of miR‐184, ‐150‐5p and ‐93‐5p were confirmed by qPCR. A reduced expression of miR‐93‐5p was confirmed in the OIR model. Conclusions This study comprehensively demonstrated the miRNA and piRNA expression profile of the aqueous humour of PDR eyes, which may serve as a potential biomarker and involved in the pathogenesis of PDR.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Chen

Yuan

Liu

et al. 2019

Clinical Exper Ophthalmology

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“…Circulating miR-126 level has been negatively correlated with fasting blood glucose and hemoglobin A1c [27]. Interestingly, in contrast to blood miR-126, our meta-analyses showed that urinary miR-126 excretion was significantly increased in patients with DN, although there was a significant heterogeneity.…”

Section: Discussionmentioning

confidence: 57%

Urinary and Blood MicroRNA-126 and -770 are Potential Noninvasive Biomarker Candidates for Diabetic Nephropathy: a Meta-Analysis

Park

Moon

Lee

et al. 2018

Cell Physiol Biochem

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Background/Aims: Diabetic nephropathy (DN), a major diabetic microvascular complication, has a long and growing list of biomarkers, including microRNA biomarkers, which have not been consistent across preclinical and clinical studies. This meta-analysis aims to identify significant blood- and urine-incident microRNAs as diagnostic/prognostic biomarker candidates for DN. Methods: PubMed, Web of Science, and Cochrane Library were searched from their earliest records through 12th Dec 2016. Relevant publications for the meta-analysis included (1) human participants; (2) microRNAs in blood and urine; (3) DN studies; and (4) English language. Four reviewers, including two physicians, independently and blindly extracted published data regarding microRNA profiles in blood and/or urine from subjects with diabetic nephropathy. A random-effect model was used to pool the data. Statistical associations between diabetic nephropathy and urinary or blood microRNA expression levels were assessed. Results: Fourteen out of 327 studies (n=2,747 patients) were selected. Blood or urinary microRNA expression data of diabetic nephropathy were pooled for this analysis. The hsa-miR-126 family was significantly (OR: 0.57; 95% CI: 0.44-0.74; p-value < 0.0001) downregulated in blood from patients with diabetic kidney disease, while its urinary level was upregulated (OR: 2931.12; 95% CI: 9.96-862623.21; p-value = 0.0059). The hsa-miR-770 family microRNA were significantly (OR: 10.24; 95% CI: 2.37-44.25; p-value = 0.0018) upregulated in both blood and urine from patients with diabetic nephropathy. Conclusions: Our meta-analysis suggests that hsa-miR-126 and hsa-miR-770 family microRNA may have important diagnostic and pathogenetic implications for DN, which warrants further systematic clinical studies.

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“…The main characteristics extracted from various datasets are summarized in Table 1, which includes the number of patients investigated, the measurement platform, type of sample used and the identity of miRNAs in the study [23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81]. Comparing results from multiple tissues provides an overall view of the impact of miRNAs in T2DM pathology.…”

Section: Resultsmentioning

confidence: 99%

A Systematic Study of Dysregulated MicroRNA in Type 2 Diabetes Mellitus

Ding

Liang

et al. 2017

IJMS

107

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MicroRNAs (miRNAs) are small noncoding RNAs that modulate the cellular transcriptome at the post-transcriptional level. miRNA plays important roles in different disease manifestation, including type 2 diabetes mellitus (T2DM). Many studies have characterized the changes of miRNAs in T2DM, a complex systematic disease; however, few studies have integrated these findings and explored the functional effects of the dysregulated miRNAs identified. To investigate the involvement of miRNAs in T2DM, we obtained and analyzed all relevant studies published prior to 18 October 2016 from various literature databases. From 59 independent studies that met the inclusion criteria, we identified 158 dysregulated miRNAs in seven different major sample types. To understand the functional impact of these deregulated miRNAs, we performed targets prediction and pathway enrichment analysis. Results from our analysis suggested that the altered miRNAs are involved in the core processes associated with T2DM, such as carbohydrate and lipid metabolisms, insulin signaling pathway and the adipocytokine signaling pathway. This systematic survey of dysregulated miRNAs provides molecular insights on the effect of deregulated miRNAs in different tissues during the development of diabetes. Some of these miRNAs and their mRNA targets may have diagnostic and/or therapeutic utilities in T2DM.

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Role of MicroRNA 126 in screening, diagnosis, and prognosis of diabetic patients in Egypt

Cited by 57 publications

References 25 publications

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Urinary and Blood MicroRNA-126 and -770 are Potential Noninvasive Biomarker Candidates for Diabetic Nephropathy: a Meta-Analysis

A Systematic Study of Dysregulated MicroRNA in Type 2 Diabetes Mellitus

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