“…[21][22][23] Of the 57 miRNAs showing concentration differences between the progressor and non-progressor groups, 26 of them (miR-10b-5p, miR-144-3p, miR-451a-5p, miR-192-5p, miR-125b-5p, miR-101-5p, miR-122-5p, miR-150-5p, miR-17-3p, miR-193a-5p, miR-193b-3p, miR-29c-5p, miR-320a-3p, miR-320b-3p, miR-550a-3p, miR-100-5p, miR-10a-5p, miR-181a-3p, miR-190a-5p, miR-136-5p, miR-375-3p, miR-487b-3p, miR-7-5p, miR-210-3p, miR-378a-3p, and miR-99a-5p) have been previously described as being associated with T2DM based on our recent meta-analysis of the field. 10 We selected 14 miRNAs for qRT-PCR validation and were able to confirm the concentration changes for 10 of these miRNAs (miR-122-5p, miR-127-5p, miR-192-5p, miR-210-3p, miR-3200-3p, miR-375-3p, miR-376b-3p, miR-4532-5p, and miR-660-3p, and miR-7641-3p). Some miRNAs could not be verified with qRT-PCR, probably due to the specific qPCR platform used and high-false negative rate associated with qRT-PCR as previously reported.…”