Role of MAPK in mechanical force-induced up-regulation of type I collagen and osteopontin in human gingival fibroblasts

Jeon, Young-Mi; Kook, Sung‐Ho; Son, Young‐Ok; Kim, Eun Mi; Park, Soon-Sun; Kim, Jong-Ghee; Lee, Jeong-Chae

doi:10.1007/s11010-008-9897-z

Cited by 42 publications

(33 citation statements)

References 32 publications

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“…This seems to indicate a degradation of cytosolic proteins without secretion to the medium. Similar effect was reported regarding the level of β-actin mRNA in HPLF and HGF cultures after centrifugation (Redlich et al 2004a, b;Jeon et al 2009). In contrast, the levels of collagen type I and collagen type I mRNA increased in these cells after centrifugation (Redlich et al 2004a, b;Jeon et al 2009).…”

Section: Discussionsupporting

confidence: 81%

“…Similar effect was reported regarding the level of β-actin mRNA in HPLF and HGF cultures after centrifugation (Redlich et al 2004a, b;Jeon et al 2009). In contrast, the levels of collagen type I and collagen type I mRNA increased in these cells after centrifugation (Redlich et al 2004a, b;Jeon et al 2009). Unfortunately protein dosage in growth medium or cell lysates of cell cultures treated by mechanical forces is not usually reported.…”

Section: Discussionsupporting

confidence: 81%

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Matrix metalloproteinase 2 activity decreases in human periodontal ligament fibroblast cultures submitted to simulated orthodontic force

Lisboa

Santos

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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Orthodontic force compresses the periodontal ligament promoting the expression of pro-inflammatory mediators and matrix metalloproteinases responsible for tooth movement. The extent in time while periodontal cells are being treated and the increment in the amount of mechanical stress caused by the orthodontic force is thought to regulate the levels of metalloproteinases in the periodontal tissue. To study the possible regulation in the activity of metalloproteinases 2, 3, 7, 9, and 10 by simulated orthodontic force, human periodontal ligament fibroblast cultures were centrifuged (141 × g) for 30, 60, 90, and 120 min, simulating the orthodontic force. Cell viability, protein quantification, and activity of metalloproteinases by zymography were evaluated at 24, 48, and 72 h after centrifugation in both cell lysates and growth medium. The activity of the 72-kDa matrix metalloproteinase 2 was decreased at 24 h regardless of the duration of centrifugation and at 48 h in cells centrifuged for 30 min only. Decrease in the amount of total protein in lysates was seen at 48 and 72 h with no change in cell viability. The data seem to indicate that the amount of mechanical stress regulates the levels of secreted matrix metalloproteinase 2. In addition, the centrifugation as a model for simulated orthodontic force may be used as a simple and reliable method to study the role played by matrix metalloproteinases in periodontal ligament when submitted to mechanical force as occurring during tooth movement.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 81%

Matrix metalloproteinase 2 activity decreases in human periodontal ligament fibroblast cultures submitted to simulated orthodontic force

Lisboa

Santos

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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“…Mechanically activated ERK1/2 and/or JNK signals are transmitted into the nucleus where they activate the transcription factor AP1 and, thereby, upregulate expression of molecules that are crucial for tissue formation and remodeling, such as type I collagen and osteopontin in bone (Hong et al, 2010;Jeon et al, 2009;Kook et al, 2009). Mechanical strain and distortion of the cell shape also activate membrane-associated phospholipases and, thereby, increase the metabolism of inositol lipids and arachidonic acid in the cytoplasm, which results in the release of Ca 2+ from intracellular stores, activation of protein kinase C (PKC) and the remodeling of cardiomyocytes through activation of mechanosensitive transcription factors, such as EGR1 and AP1 (Fig.…”

Section: Mechanosensitive Transmembrane Receptorsmentioning

confidence: 99%

Mechanosensitive mechanisms in transcriptional regulation

Mammoto

Ingber

2012

Journal of Cell Science

342

291

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SummaryTranscriptional regulation contributes to the maintenance of pluripotency, self-renewal and differentiation in embryonic cells and in stem cells. Therefore, control of gene expression at the level of transcription is crucial for embryonic development, as well as for organogenesis, functional adaptation, and regeneration in adult tissues and organs. In the past, most work has focused on how transcriptional regulation results from the complex interplay between chemical cues, adhesion signals, transcription factors and their co-regulators during development. However, chemical signaling alone is not sufficient to explain how three-dimensional (3D) tissues and organs are constructed and maintained through the spatiotemporal control of transcriptional activities. Accumulated evidence indicates that mechanical cues, which include physical forces (e.g. tension, compression or shear stress), alterations in extracellular matrix (ECM) mechanics and changes in cell shape, are transmitted to the nucleus directly or indirectly to orchestrate transcriptional activities that are crucial for embryogenesis and organogenesis. In this Commentary, we review how the mechanical control of gene transcription contributes to the maintenance of pluripotency, determination of cell fate, pattern formation and organogenesis, as well as how it is involved in the control of cell and tissue function throughout embryogenesis and adult life. A deeper understanding of these mechanosensitive transcriptional control mechanisms should lead to new approaches to tissue engineering and regenerative medicine. IntroductionTissue and organ architecture are constructed as a result of the complex orchestration of various cell types that exhibit specific behaviors (e.g. growth, differentiation, migration and apoptosis) in specific locations at precise times during embryogenesis. Transcriptional programs confer and maintain cellular identity and functions that are necessary for the maturation of embryonic cells into terminally differentiated tissues or organs, as well as for stem cell fate switching throughout adult life. These behaviors are regulated by turning genes on and off in a highly spatiotemporally controlled manner, and this process is mediated at the level of gene transcription through the interplay between RNA polymerase II, various transcription factors and their co-regulators (Box 1). Whereas most studies on transcriptional regulation have focused on genetic or chemical control mechanisms of transcription, recent work suggests that mechanical forces (Box 2) are equally important regulators of transcriptional control in embryonic and adult tissues. In this Commentary, we review these new insights into cellular mechanotransduction, and discuss how mechanical cues influence transcriptional regulation to govern organogenesis and to ensure tissue maintenance throughout adult life.

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“…Primary cultures of hGF and hPLF were also used as control cells (20,21). This study was approved by the Review Board of Chonbuk National University Hospital (CNUH).…”

Section: Cell Cultures and Papu Treatmentmentioning

confidence: 99%

Role of MAPK in mechanical force-induced up-regulation of type I collagen and osteopontin in human gingival fibroblasts

Cited by 42 publications

References 32 publications

Matrix metalloproteinase 2 activity decreases in human periodontal ligament fibroblast cultures submitted to simulated orthodontic force

Matrix metalloproteinase 2 activity decreases in human periodontal ligament fibroblast cultures submitted to simulated orthodontic force

Mechanosensitive mechanisms in transcriptional regulation

Induction of caspase-dependent apoptosis in melanoma cells by the synthetic compound (E)-1-(3,4-dihydroxyphenethyl)-3-styrylurea

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