Role of &lt;i&gt;Pneumocysti&lt;/i&gt;&lt;i&gt;s&lt;/i&gt;&lt;i&gt;carinii&lt;/i&gt; DNA Amplification by PCR on the Diagnosis of &lt;i&gt;Pneumocystis&lt;/i&gt;&lt;i&gt;carinii&lt;/i&gt;&lt;i&gt;Pneumonia&lt;/i&gt; in Patients with Haematologic Malignant Diseases: Report of Four Cases

Antinori, Andrea; Pagano, Livio; Luca, Andrea De; Marra, R; Mencarini, Paola; Tamburrini, Enrica

doi:10.1159/000204002

Cited by 3 publications

(2 citation statements)

References 12 publications

(19 reference statements)

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“…• polymerase chain reaction (PCR), from induced sputum and BAL fluid, using primers for the human P. carinii mitochondrial large‐subunit (mtLSU) rRNA gene (Antinori et al , 1995) and/or…”

Section: Methodsmentioning

confidence: 99%

“…• visualization of P. carinii (PC) trophoozoites (trophic form) from sputum or bronchoalveolar (BAL) fluid, using either a modified Wright-Giemsa or a Papanicolaou stain, and cysts stained with toluidine blue O stain (Naimey & Wuerker, 1995) and/or • polymerase chain reaction (PCR), from induced sputum and BAL fluid, using primers for the human P. carinii mitochondrial large-subunit (mtLSU) rRNA gene (Antinori et al, 1995) and/or • immunostaining using monoclonal antibodies (mAbs) for human P. carinii in sputum, nasopharyngeal aspirates, BAL fluid or lung tissue obtained by transbronchial biopsy or open lung biopsy (Kovacs et al, 1988) and/or • histological documentation of tissue invasion by PC cysts or trophoozoites in lung tissue obtained by transbronchial biopsy or by open lung biopsy or at autopsy (Thomas & Limper, 1998).…”

Section: Methodsmentioning

confidence: 99%

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Pneumocystis carinii pneumonia in patients with malignant haematological diseases: 10 years' experience of infection in GIMEMA centres

et al. 2002

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Summary. A retrospective survey was conducted over a 10-year period (1990-99) among 52 haematology divisions in order to evaluate the clinical and laboratory characteristics and outcome of patients with proven Pneumocystis carinii pneumonia (PCP) complicating haematological diseases. The study included 55 patients (18 with non-Hodgkin's lymphoma, 10 with acute lymphoblastic leukaemia, eight with acute myeloid leukaemia, five with chronic myeloid leukaemia, four with chronic lymphocytic leukaemia, four with multiple myeloma, three with myelodysplastic syndrome, two with myelofibrosis and one with thalassemia) who developed PCP. Among these, 18 (33%) underwent stem cell transplantation; only two received an oral prophylaxis with trimethroprim/sulphamethoxazole. Twelve patients (22%) developed PCP despite protective isolation in a laminar airflow room. The most frequent symptoms were: fever (86%), dyspnoea (78%), non-productive cough (71%), thoracic pain (14%) and chills (5%); a severe hypoxaemia was present in 39 patients (71%). Chest radiography or computerized tomography showed interstitial infiltrates in 34 patients (62%), alveolar infiltrates in 12 patients (22%), and alveolar-interstitial infiltrates in nine patients (16%). Bronchoalveolar lavage was diagnostic in 47/48 patients, induced sputum in 9/18 patients and lung biopsy in 3/8 patients. The diagnosis was made in two patients at autopsy. All patients except one started a specific treatment (52 patients trimethroprim/sulphamethoxazole, one pentamidine and one dapsone). Sixteen patients (29%) died of PCP within 30 d of diagnosis. Multivariate analysis showed that prolonged steroid treatment (P < 0AE006) and a radiological picture of diffuse lung involvement (P < 0AE003) were negative diagnostic factors.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Pneumocystis carinii pneumonia in patients with malignant haematological diseases: 10 years' experience of infection in GIMEMA centres

et al. 2002

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Pulmonary fungal infection with yeasts andpneumocystisin patients with hematological malignancy

2005

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Invasive fungal infections are an important cause of morbidity and mortality in patients with hematological malignancies, and in particular fungal pneumonia is the main clinical manifestation in this category of patients. The fungal agents responsible for this complication are various, but Aspergillus spp. and other molds such as Zygomycetes or Fusarium spp. represent the most frequently isolated micro-organisms. Less commonly, pneumonia could be due to other 'no-molds' fungal agents such as Candida spp, Cryptococcus spp, or Pneumocystis jirovecii . This review mainly focuses on practical aspects relevant to epidemiology, diagnosis and therapeutic management of the rare cases of pneumonia due to no-molds agents in patients affected by hematological malignancies.

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The Role of Bronchoalveolar Lavage in the Microbiological Diagnosis of Pneumonia in Patients with Haematological Malignancies

et al. 1997

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In the aetiological diagnosis of pulmonary infections in patients affected by haematological malignancies we evaluated the utility of bronchoalveolar lavage (BAL). One hundred and twenty-seven BAL were performed in 119 patients. In our series, we identified the agent of pneumonia in 53.5% of episodes with the best results in aspergillosis, very common in these patients. The previous empirical anti-infective treatment was modified in 14 episodes (11%). The procedure was generally well tolerated and only one patient bled. We maintain that BAL is a useful diagnostic tool for detecting the agents of pulmonary infections in patients with haematological malignancies, especially when the routine microbiological procedures fail, and it also represents a good alternative to more invasive procedures.

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Role of <i>Pneumocysti</i><i>s</i><i>carinii</i> DNA Amplification by PCR on the Diagnosis of <i>Pneumocystis</i><i>carinii</i><i>Pneumonia</i> in Patients with Haematologic Malignant Diseases: Report of Four Cases

Cited by 3 publications

References 12 publications

Pneumocystis carinii pneumonia in patients with malignant haematological diseases: 10 years' experience of infection in GIMEMA centres

Pneumocystis carinii pneumonia in patients with malignant haematological diseases: 10 years' experience of infection in GIMEMA centres

Pulmonary fungal infection with yeasts andpneumocystisin patients with hematological malignancy

The Role of Bronchoalveolar Lavage in the Microbiological Diagnosis of Pneumonia in Patients with Haematological Malignancies

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