Role of leukocyte plugging and edema in skeletal muscle ischemia-reperfusion injury

Harris, A. G.; Steinbauer, Markus; Leiderer, Rosmarie; Meßmer, K.

doi:10.1152/ajpheart.1997.273.2.h989

Cited by 35 publications

(37 citation statements)

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“…The dorsal skinfold chamber is a widely used and well-accepted model in microcirculatory research in hamsters and mice. [12][13][14][15][16][17][18] Scaffold manufacturing and characteristics A block-copolymer polymer composed of alternating soft blocks of polyethylene glycol terephthalate (PEGT) and hard blocks of polybutylene terephthalate (PBT) was used. Polymer composition can be modified by varying the weight percentages of PEGT and PBT or by changing the molecular weight (MW) of the PEG.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Druecke

Langer

Lamme³

et al. 2003

J Biomedical Materials Res

169

138

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Poly(ether ester) block-copolymer scaffolds of different pore size were implanted into the dorsal skinfold chamber of balb/c mice. Using intravital fluorescent microscopy, the temporal course of neovascularization into these scaffolds was quantitatively analyzed. Three scaffold groups (diameter, 5 mm; 220 -260 thickness, m; n ϭ 30) were implanted. Different pore sizes were evaluated: small (20 -75 m), medium (75-212 m) and large pores (250 -300 m). Measurements were performed on days 8, 12, 16, and 20 in the surrounding normal tissue, in the border zone, and in the center of the scaffold. Standard microcirculatory parameters were assessed (plasma leakage, vessel diameter, red blood cell velocity, and functional vessel density). The largepored scaffolds showed significantly higher functional vessel density in the border zone and in the center (days 8 and 12) compared with the scaffold with the small and mediumsized pores. These data correlated with a larger vessel diameter and a higher red blood cell velocity in the largepored scaffold group. Interestingly, during the evaluation period the microcirculatory parameters on the edge of the scaffolds returned to values similar to those found in the surrounding tissue. In the center of the scaffold, however, neovascularization was still active 20 days after implantation. Plasma leakage and vessel diameter were higher in the center of the scaffold. Red blood cell velocity and functional vessel density were 50% lower than in the surrounding tissue. In conclusion, the dorsal skinfold chamber model in mice allows long-term study of blood vessel growth and remodeling in porous biomedical materials. The rate of vessel ingrowth into poly(ether ester) block-copolymer scaffolds is influenced by pore size and was highest in the scaffold with the largest pores. The data generated with this model contribute to knowledge about the development of functional vessels and tissue ingrowth into biomaterials.

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Section: Surgical Proceduresmentioning

confidence: 99%

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Druecke

Langer

Lamme³

et al. 2003

J Biomedical Materials Res

169

138

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“…4c and d). In addition, individual necrotic cell death can be detected by the use of propidium-iodide (Harris et al, 1997), whereas apoptotic cells can be identifi ed by nuclear condensation, margination and fragmentation following local bisbenzimide staining (Vollmar et al, 2001). Besides conventional epi-illumination fl uorescence microscopy, imaging in the dorsal skinfold chamber model has increasingly been performed in recent years by means of confocal microscopy techniques (Isaka et al, 2004;Makale, 2007;Strieth et al, 2008).…”

Section: Mw Laschke Et Almentioning

confidence: 99%

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

143

128

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The implantation of biomaterials into the human body has become an indispensable part of almost all fi elds of modern medicine. Accordingly, there is an increasing need for appropriate approaches, which can be used to evaluate the suitability of different biomaterials for distinct clinical indications. The dorsal skinfold chamber is a sophisticated experimental model, which has been proven to be extremely valuable for the systematic in vivo analysis of the dynamic interaction of small biomaterial implants with the surrounding host tissue in rats, hamsters and mice. By means of intravital fl uorescence microscopy, this chronic model allows for repeated analyses of various cellular, molecular and microvascular mechanisms, which are involved in the early infl ammatory and angiogenic host tissue response to biomaterials during the initial 2-3 weeks after implantation. Therefore, the dorsal skinfold chamber has been broadly used during the last two decades to assess the in vivo performance of prosthetic vascular grafts, metallic implants, surgical meshes, bone substitutes, scaffolds for tissue engineering, as well as for locally or systemically applied drug delivery systems. These studies have contributed to identify basic material properties determining the biocompatibility of the implants and vascular ingrowth into their surface or internal structures. Thus, the dorsal skinfold chamber model does not only provide deep insights into the complex interactions of biomaterials with the surrounding soft tissues of the host but also represents an important tool for the future development of novel biomaterials aiming at an optimisation of their biofunctionality in clinical practice.

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“…Free radicals and cytokines generated by IR injury trigger neutrophil recruitment and adhesion, capillary plugging, endothelial cell swelling, and the eventual collapse of the capillary lumen, leading to a phenomenon called capillary no-reflow. 36,60 Capillary no-reflow results in arterial venous shunting, 42 and a significant reduction of nutritive blood flow within the affected capillary. Importantly, arterial venous shunting and collapsed capillaries have been observed in the limbs of patients with CRPS.…”

Section: Role Of Microvascular Dysfunctionmentioning

confidence: 99%

Complex Regional Pain Syndrome: What's in a Name?

Coderre

2011

The Journal of Pain

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Within a two year period in the 1940's, two Boston physicians published dramatically opposing views on the underlying nature of a syndrome now known as complex regional pain syndrome (CRPS). Evans suggested, in several papers in [1946][1947], that sympathetic reflexes maintain pain and dystrophy in affected limbs. Foisie, in 1947, suggested arterial vasospasms were key in the etiology of this pain syndrome. Evans' hypothesis established the nomenclature for this syndrome for 60 years, and his term "reflex sympathetic dystrophy" guided clinical treatment and research activities over the same period. Foisie's proposed nomenclature was unrecognized, and had virtually no impact on the field. Recent evidence suggests that Evans' contribution to the field may have in fact lead clinicians and researchers astray all those years. This focus article on CRPS compares recent observations with these 2 earlier theories and asks the question --what if we had adopted Foisie's nomenclature from the beginning?

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Role of leukocyte plugging and edema in skeletal muscle ischemia-reperfusion injury

Cited by 35 publications

References 0 publications

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

Neovascularization of poly(ether ester) block‐copolymer scaffolds in vivo: Long‐term investigations using intravital fluorescent microscopy

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Complex Regional Pain Syndrome: What's in a Name?

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