Role of lactone ring of aflatoxin B1 in toxicity and mutagenicity

Lee, L. S.; Dunn, John J.; DeLucca, Anthony J.; Ciegler, A.

doi:10.1007/bf01965543

Cited by 96 publications

(58 citation statements)

References 11 publications

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“…Chromophore compound of AFB1 is able to be detected by fluorescence-based detection method. However the toxic moieties of AFB1 do not have fluorescence properties (Lee et al, 1981). Therefore, AFB1 reduction product had been also confirmed with bio-analysis related to its safety.…”

Section: Detoxification Of Afb1 By Aspergillus Oryzae Kkb4mentioning

confidence: 94%

The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

Carolina¹,

Setyabudi²,

Sardjono³

2017

MJM

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Aims:The growth and metabolic activity of Aspergillus oryzae KKB4 in AFB1-contaminated corn and later coincided with AFB1 reduction and detoxification were investigated. Methodology and results:The decreasing of AFB1 amount by A. oryzae KKB4 could be clearly observed if the initial AFB1 concentration in corn was increased. Thus, moist-heated corn was artificially inoculated with Aspergillus flavus FNCC 62C7 to increase AFB1 content. AFB1-contaminated corn was applied as solid substrate and then inoculated with A. oryzae KKB4. During fermentation periods, the growth, metabolic activity, and AFB1 decline were investigated by glucosamine content, water content loss, and AFB1 concentration, respectively. The maximum growth was occurred in 4 th day at 1.499 ± 0.028 g glucosamine/ 100 g dry matter. The metabolic activity was going on up to the end of fermentation days, as shown as water content loss at 0.175 ± 0.007 g/g dry matter. In accordance with the growth and metabolic activity, the amount of AFB1 reduction was 37.04 ng AFB1/g dry matter during 5 days fermentation. According to toxicity analysis, it was found that the residues of AFB1 were not toxic to Bacillus megaterium cells. Conclusion, significance and impact of study: A. oryzae KKB4 is able to be applied in solid substrates as AFB1 reduction and detoxification agent. These lucrative effects are also important in relation with food and feed safety.

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Section: Detoxification Of Afb1 By Aspergillus Oryzae Kkb4mentioning

confidence: 94%

The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

Carolina¹,

Setyabudi²,

Sardjono³

2017

MJM

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“…48). The mutagenicity (Ames test-TA100) and toxicity (chick embryo test) of aflatoxin D I compared to aflatoxin BI are reduced by 450-fold and 18-fold, respectively (Lee et al, 1981;Park et al, 1988). This process effectively decontaminates aflatoxin-containing feeds.…”

Section: Aflatoxin Absorption and Removal From Feeds And Foodsmentioning

confidence: 96%

Natural Toxicants in Foods

Beier

Nigg

1992

Phytochemical Resources for Medicine and Agriculture

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“…La présence d'une double liaison sur le cycle dihydrofurane de l'AFB1 et de l'AFG1 serait à l'origine de la différence de toxicité avec leurs homologues AFB2 et AFG2. Par contre la différence de toxicité entre les aflatoxines du groupe B et G proviendrait de la substitution du cycle cyclopentone par un cycle lactone (Lee et al, 1981). Ces aflatoxines sont structurellement proches constitués par un assemblage d'une coumarine et de 3 furanes.…”

Section: Description Et Origine Des Aflatoxinesunclassified

Contamination des céréales par l’aflatoxine en Afrique : revue des méthodes de lutte existantes

Diémé¹,

Fall²,

Sarr³

et al. 2017

Int. J. Bio. Chem. Sci

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RESUME Les aflatoxines sont des toxines produites principalement par les moisissures du genre Aspergillus (Aspergillus flavus et Aspergillus parasiticus).Les molécules les plus abondantes de la famille des aflatoxines sont l'AFB1, l'AFB2, l'AFG1 et l'AFG2. C'est dans les pays africains, d'Asie du Sud et d'Amérique du Sud, où le climat est chaud et humide, que la contamination des céréales par l'aflatoxine est plus fréquente. Dans la plus part des pays africains, près de la moitié de la production céréalière a une teneur en aflatoxine supérieure aux standards internationaux. L'estimation de la quantité d'aflatoxine dans le maïs en 2016 a donné des intervalles de [1,06-852,2ppm] au Sénégal, de [8,0-1081ppm] en Tanzanie, avec un taux d'échantillons dépassant la limite maximale qui varie entre 2-85% de 622 échantillons. Au niveau africain la contamination des céréales par cette toxine a des conséquences économiques, alimentaires et surtout sanitaires. Pour lutter contre la présence de l'aflatoxine dans des céréales en Afrique des méthodes chimiques, physiques et biologiques sont employées, bien qu'elles soient limitées. © 2016 International Formulae Group. All rights reserved.Mot clés : Aflatoxine, Contamination, Céréale, Lutte, Prévalence, Afrique. African crops contamination by aflatoxin: review of existing methods of control ABSTRACTAflatoxins are toxins produced mainly by Aspergillus (Aspergillus flavus and Aspergillus parasiticus). The most abundant molecules of aflatoxins family are AFB1, AFB2, AFG1 and AFG2. It is in African countries, South Asia and South America, where the climate is hot and humid, that contamination of crops with aflatoxin is more common. In most African countries, nearly half of crops production has higher aflatoxin content compared to international standards. The estimate of the quantity of aflatoxin in crops on the year 2016 E. DIEME et al. / Int. J. Biol. Chem. Sci. 10(5): 2285-2299, 2016 2286 has yielded intervals [1. 06-852.2ppb] in Senegal, [8.0-1081ppb] in Tanzania, with a sample rate exceeding the maximum limit that varies between 2-85% of 622 samples. The level of grain aflatoxin contamination in Africa has economic and food consequences; and especially impact the human health. To fight against the contamination of Africa crops by aflatoxin, physical, chemical and biological methods are employed with obvious limits.

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Role of lactone ring of aflatoxin B1 in toxicity and mutagenicity

Abstract: Cleavage of the lactone ring of aflatoxin B1 results in a nonfluorescent compound that has greatly reduced biological activity. Mutagenicity, as measured by the Ames test, is reduced 450-fold compared to that of B1, and toxicity, as measured by the chick embryo test, is reduced 18-fold.

Cited by 96 publications

References 11 publications

The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

Natural Toxicants in Foods

Contamination des céréales par l’aflatoxine en Afrique : revue des méthodes de lutte existantes

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