Role of Iron in the Suppression of Bacterial Plant Pathogens by Fluorescent Pseudomonads

Bakker, Peter A. H. M.; Raaijmakers, J.M.; Schippers, B.

doi:10.1016/b978-0-12-079870-4.50017-x

Cited by 25 publications

(11 citation statements)

References 54 publications

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“…Efficient nutrient scavenging systems, combined with an ability to utilize a diverse array of nutrient sources, are likely to be crucial for competitive ability in the rhizosphere (Bakker et al ., 1993; Bolton et al ., 1993; Oresnik et al ., 1998). Studies of exudate collected from roots show the presence of various sugars, purines, nucleosides, organic acids, amino acids and vitamins (including pantothenate) (Rovira, 1969).…”

Section: Discussionmentioning

confidence: 99%

Adaptation of Pseudomonas fluorescens to the plant rhizosphere

Rainey

1999

Environmental Microbiology

341

336

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Saprophytic Pseudomonas are common root-colonizing bacteria that can improve plant health. Efficient exploitation of these bacteria in agriculture requires knowledge of traits that enhance ecological performance in the rhizosphere. Here, I describe the development and application of a promoter-trapping technology (IVET) that enables the isolation of Pseudomonas fluorescens genes that show elevated levels of expression in the rhizosphere. Using IVET, 20 P. fluorescens genes were identified that are induced during rhizosphere colonization, and their patterns of expression were analysed in laboratory media and in the rhizosphere. Fourteen genes showed significant homology to sequences in GenBank that are involved in nutrient acquisition, stress response, or secretion; six showed no homology. Seven of the rhizosphere-induced (rhi) genes have homology to known non-Pseudomonas genes. One of the rhi genes (hrcC) is a component of a type III secretion pathway, not previously known in non-parasitic bacteria. Together, these genes provide a view of the rhizosphere environment as perceived by a rhizosphere colonist, and suggest that the nature of the association between P. fluorescens and the plant root may be more complex and intimate than previously thought.

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Section: Discussionmentioning

confidence: 99%

Adaptation of Pseudomonas fluorescens to the plant rhizosphere

Rainey

1999

Environmental Microbiology

341

336

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“…Pseudomonas putida WCS358 is a PGPR originally isolated from the rhizosphere of potato (Geels and Schippers, 1983a, b). It can suppress soil‐borne plant diseases by siderophore‐mediated competition for iron (Bakker et al ., 1986, 1993). WCS358 can also induce systemic resistance in Arabidopsis thaliana against Pseudomonas syringae pv.…”

Section: Introductionmentioning

confidence: 99%

Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants

Meziane

Sluis

Loon

et al. 2005

Molecular Plant Pathology

287

173

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SUMMARY Pseudomonas putida WCS358 is a plant growth-promoting rhizobacterium originally isolated from the rhizosphere of potato. It can suppress soil-borne plant diseases by siderophore-mediated competition for iron, but it has also been reported to result in induced systemic resistance (ISR) in Arabidopsis thaliana. Bacterial determinants of this strain involved in inducing systemic resistance in Arabidopsis were investigated using a Tn5 transposon mutant defective in biosynthesis of the fluorescent siderophore pseudobactin, a non-motile Tn5 mutant lacking flagella, and a spontaneous phage-resistant mutant lacking the O-antigenic side chain of the lipopolysaccharides (LPS). When using Pseudomonas syringae pv. tomato as the challenging pathogen, purified pseudobactin, flagella and LPS all triggered ISR. However, the mutants were all as effective as the parental strain, suggesting redundancy in ISR-triggering traits in WCS358. The Botrytis cinerea-tomato, B. cinerea-bean and Colletotrichum lindemuthianum-bean model systems were used to test further the potential of P. putida WCS358 to induce ISR. Strain WCS358 significantly reduced disease development in all three systems, indicating that also on tomato and bean WCS358 can trigger ISR. In both tomato and bean, the LPS mutant had lost the ability to induce resistance, whereas the flagella mutant was still effective. In bean, the pseudobactin mutant was still effective, whereas this mutant has lost its effectivity in tomato. In both bean and tomato, flagella isolated from the parental strain were not effective, whereas LPS or pseudobactin did induce systemic resistance.

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“…These siderophores are sought to deprive pathogens of iron, copper, and/or zinc, thus suppress their growth (HAAS & DÉFAGO, 2005). Pseudomonas putida WCS358 was found to be able to produce pseudobactin, and via the siderophore-mediated competition for iron it could suppress soil-borne plant diseases (BAKKER et al, 1993;MEZIANE et al, 2005). MANNINEN and MATTILA-SANDHOLM (1994) detected Pseudomonas siderophores by analysing the absorption spectrum, and observed that both P. putida strains tested had an absorbance peak at 400 nm.…”

Section: Antagonistic Effect Of the Isolates On L Monocytogenesmentioning

confidence: 99%

Endophytic bacteria fromCapsicum annuumvar.grossumcultivars and their inhibitory effects onListeria monocytogenes

et al. 2014

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Endophytic microorganisms living inside plant tissues might have numerous positive effects on the host plants. Endophytes can promote the growth and yield of the plant, help to remove contaminants from the tissues, and can suppress growth of pathogens; however, some enteric human pathogenic bacteria have also been isolated as endophytes. The aims of our study were the characterisation and identifi cation of endophytic coliform bacteria isolated from different cultivars of sweet pepper (Capsicum annuum var. grossum) using a selective (VRBL) agar medium, and determination of antagonistic interactions between these endophytes and Listeria monocytogenes. The bacterial isolates showed heterogeneity based on their phenotypic and genotypic properties. Results of identifi cation by molecular biological methods also confi rmed the presence of different genera/species. When the antagonistic effect of the isolated endophytic bacteria was tested it was found that one isolate -identifi ed as Pseudomonas putida -showed signifi cant inhibition on the growth of Listeria monocytogenes.

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Role of Iron in the Suppression of Bacterial Plant Pathogens by Fluorescent Pseudomonads

Cited by 25 publications

References 54 publications

Adaptation of Pseudomonas fluorescens to the plant rhizosphere

Adaptation of Pseudomonas fluorescens to the plant rhizosphere

Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants

Endophytic bacteria fromCapsicum annuumvar.grossumcultivars and their inhibitory effects onListeria monocytogenes

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