Endophytic bacteria fromCapsicum annuumvar.grossumcultivars and their inhibitory effects onListeria monocytogenes

Abstract

Endophytic microorganisms living inside plant tissues might have numerous positive effects on the host plants. Endophytes can promote the growth and yield of the plant, help to remove contaminants from the tissues, and can suppress growth of pathogens; however, some enteric human pathogenic bacteria have also been isolated as endophytes. The aims of our study were the characterisation and identifi cation of endophytic coliform bacteria isolated from different cultivars of sweet pepper (Capsicum annuum var. grossum) using a selective (VRBL) agar medium, and determination of antagonistic interactions between these endophytes and Listeria monocytogenes. The bacterial isolates showed heterogeneity based on their phenotypic and genotypic properties. Results of identifi cation by molecular biological methods also confi rmed the presence of different genera/species. When the antagonistic effect of the isolated endophytic bacteria was tested it was found that one isolate -identifi ed as Pseudomonas putida -showed signifi cant inhibition on the growth of Listeria monocytogenes.

Colonization ability of Escherichia coli and Listeria monocytogenes in the endosphere of sweet pepper (Capsicum annuum var. grossum)

“…Seeds of Karpia cultivar of sweet pepper (Capsicum annuum L. var. grossum) were surfacedisinfected (BELÁK et al, 2014) and inoculated with E. coli ATCC 8739 and L. monocytogenes CCM 4699 separately, as described in NIRANJAN RAJ and co-workers (2003). Non-inoculated seeds served as sterile controls, while non-disinfected ones without bacterization were used as non-sterile controls.…”