Role of interleukin-1 in augmenting serum neutralization of bacterial lipopolysaccharide

Riveau, G R; Novitsky, Thomas J.; Roslansky, Priscilla F.; Dinarello, Charles A.; Warren, H. Shaw

doi:10.1128/jcm.25.5.889-892.1987

Cited by 29 publications

(5 citation statements)

References 16 publications

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“…These proteins could inhibit the synthesis or neutralize the action of mediators involved in the genesis of fever, and the treatment with galactosamine, simultaneously with the first injection of MDP or endotoxin, would inhibit their synthesis and consequently the development of pyrogenic tolerance. In support of this hypothesis, it has been proposed that a seric component, probably of hepatic origin, neutralizes endotoxin (29,41) and that serum with a high concentration of APP reduces the lethality induced by endotoxin (38). Directly related to the phenomenon investigated in the present study, it has been demonstrated that serum amyloid A, an APP produced by the liver, inhibits fever and hypothalamic production of prostaglandin E 2 induced by cytokines in mice (36).…”

Section: Discussionmentioning

confidence: 99%

“…Beeson (2) and Cooper and Cranston (4) suggested that the tolerance to the pyrogenic effect of endotoxin would result from an increase of its clearance by the reticuloendothelial system resulting in a faster elimination of endotoxin from the circulation. Tolerant animals may also produce a seric component that neutralizes endotoxin, as suggested by Riveau et al (29) and Warren et al (41). However, the aspect that has been most investigated to explain the development of tolerance is the production of the pyrogenic cytokines.…”

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confidence: 99%

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Role of the hepatic function in the development of the pyrogenic tolerance to muramyl dipeptide

Ferreira

Coelho

Pelá

2001

American Journal of Physiology-Regulatory, Integrative and Comp

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We have demonstrated that the hepatic function may have an important role in the development of tolerance to the pyrogenic effect induced by endotoxin. To further investigate if the role of the hepatic function in the development of tolerance also extends to that induced by other pyrogenic stimuli, we investigated the effect of galactosamine, a specific inhibitor of the hepatic protein synthesis, on the development of tolerance to the pyrogenic effect induced by muramyl dipeptide (MDP) in rats. Pyrogenic tolerance was observed after the second intravenous or intraperitoneal injection of MDP (500 microgram/kg), 24 h after the first injection, similar to what was observed with endotoxin. Pyrogenic tolerance was abolished when galactosamine (300 mg/kg ip) was injected simultaneously with MDP (500 microgram/kg iv) on the first day. When uridine (600 mg/kg ip) was administered simultaneously with galactosamine (300 mg/kg ip) and the first injection of MDP (500 microgram/kg ip), pyrogenic tolerance was again observed after the second injection of the peptidoglycan. In conclusion, the hepatic function may not be important only for the development of tolerance to endotoxin, but also to a totally different pyrogenic stimulus such as MDP.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Role of the hepatic function in the development of the pyrogenic tolerance to muramyl dipeptide

Ferreira

Coelho

Pelá

2001

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Tìm kiếm thuốc kháng nội độc tố Trong nghiên cứu nhiễm trùng gram âm và nhiễm trùng huyết, nội độc tố đóng một vai trò quan trọng trong đáp ứng sinh lý bệnh của vật chủ [24]. Giả định rằng nếu các tác động bất lợi của nội độc tố có thể được loại trừ, khả năng sống trong bệnh nhiễm khuẩn có thể được cải thiện.…”

Section: ứNg Dụng Của Lal Trong Nghiên Cứu Y Dược Họcunclassified

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Bui

Hải²,

Huyen³

2018

MPS

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Biomemetics (or bionics) as a scientific discipline has potential applications in various research fields as well as in improving human lives. In the field of medicine and pharmacy, biomimetic methods are of great value in developing drugs, developing methods for the diagnosis, prevention and treatment of diseases. Biomimetics of immune system in medicine and pharmacy is a great subject with the potential to make remarkable progress. The immune system of the species is rich, diverse and has different mechanisms. This study investigates the biomimetics of Limulus immune system and its practical application prospects.

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“…In another study we found that sera drawn from rabbits made tolerant to smooth LPS neutralize more heterologous LPS than do normal sera (33). Further investigation has revealed that injection of macrophage supernatants containing interleukin-1 and recombinant interleukin-1 induces increased serum neutralization of LPS (21). In light of these experiments and the controversies surrounding the protective mechanism(s) of passively transferred antisera to gram-negative organisms, we used the limulus inhibition assay to study the amounts of LPS neutralized by rabbit antisera directed to rough and smooth gram-negative organisms.…”

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confidence: 94%

Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other gram-negative bacteria

Warren¹,

Novitsky²,

Bucklin³

et al. 1987

Infect Immun

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To study the mechanisms of protection against endotoxin challenge offered by antisera to smooth and rough gram-negative organisms, we have developed an assay to quantitate endotoxin neutralization based on inhibition of the Limulus amoebocyte lysate test. Dilutions of different bacterial lipopolysaccharides (LPSs) were incubated with hyperimmune rabbit sera against Escherichia coli 0113, E. coli 018, and rough mutants E. coli J5 and Salmonella minnesota Re595 and were then combined with limulus lysate. The gelation reaction induced by LPS in the lysate was monitored spectrophotometrically, and the concentration of LPS resulting in a 50% lysate response was determined and correlated with antibody titers measured by enzyme-linked immunosorbent assay. Antisera to smooth organisms neutralized homologous LPS markedly and heterologous LPSs only minimally relative to neutralization by preimmune serum. Neutralization of homologous LPS occurred immediately without preincubation of serum and LPS. Antisera to rough mutants neutralized more heterologous LPS than did antisera to smooth organisms. However, this heterologous neutralization required preincubation of serum and LPS and did not appear to be correlated with antibody concentrations. We conclude that antisera to LPS rapidly neutralize the biological activity of the homologous LPS, as detected by limulus lysate, and that neutralization is at least in part antibody mediated. Antisera to rough-mutant organisms slowly neutralized the activity of heterologous LPSs, but this effect appeared not to be correlated with concentrations of antibody to the LPS of the rough mutant, as measured by enzyme-linked immunosorbent assay.

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Role of interleukin-1 in augmenting serum neutralization of bacterial lipopolysaccharide

Abstract: We have previously described an assay to quantify the serum neutralization of bacterial lipopolysaccharide which is based on a spectrophotometric Limulus amoebocyte lysate test (T.

Cited by 29 publications

References 16 publications

Role of the hepatic function in the development of the pyrogenic tolerance to muramyl dipeptide

Role of the hepatic function in the development of the pyrogenic tolerance to muramyl dipeptide

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Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other gram-negative bacteria

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