Role of interferon in mice in protection against influenza A virus by bacterial ribosomes together with membranal glycoproteins of Klebsiella pneumoniae as adjuvant

Abstract

Nonspecific protection against infectious aerosols of influenza A virus was obtained in Swiss mice after vaccination by aerosols of bacterial ribosomes together with membranal glycoproteins extracted from Klebsiella pneumoniae as the adjuvant. It was shown that repeated stimulant aerosols were necessary to obtain this protection. Routine estimation of serum interferon levels after administration of the association of ribosomes plus membranal glycoproteins to the animals by aerosol or intravenous route showed that there was no correlation between protection and the presence of serum interferon. It was shown that the serum interferon-inducer activity was due to ribosomes. No induction of serum interferon was obtained with membranal glycoproteins used separately. Local liberation of interferon in the mucous membrane of the upper respiratory tract was not investigated.

Requirement of the conformational stability of a Salmonella ribosomal vaccine for its mouse protection

“…Butler et al [30] reported that nuclease digestion of salmonella ribosomal preparations destroyed their ability to stimulate the production of immuno-enhancing factors in serum of treated mice. Another report showed that bacterial RNA contributes to the serum interferon-inducer activity [31]. These reports implicate the important role of RNA in producing the helper factor(s) including interleukins, as previously reported [32].…”

Innate Immune Signaling Activated by MDR Bacteria in the Airway

“…This may be clinically relevant as subtherapeutic concentrations of antibiotics actually increase production of capsular proteins (166), an observation that has been made in other pathogens of the carbapenemase resistant Enterobacteriaceae (CRE) family (137). Furthermore, upregulation of interferons has long been known to be increased in response to capsular proteins (198,199), which have been used as an adjuvant to help clear airway infection with influenza virus (347).…”