Role of immunohistochemical markers in breast cancer and their correlation with grade of tumour, our experience

Abstract

Objectives: To study the immunohistochemical markers in invasive carcinoma of breast and to assess the relationship of hormonal receptors and Her2Neu oncoprotein with grade of tumour. Materials and methods: This was a comparative cross sectional study conducted at Chughtais Lahore lab from Jan 2011-Oct 2011. Eighty two biopsy proven cases of breast carcinoma were reviewed. Immunohistochemistry was used to evaluate the expression of Estrogen receptors(ER) and progesterone receptors (PR) and Her2Neu oncoprotein. Grade of tumour was assessed by Scarf Bloom Richerdson Grading system. Results: This study comprised 82 cases of invasive breast carcinoma. Out of these 82 cases, 2 were males and 80 were females. Majority of the cases were between 25-45 years age group. Fifty seven cases (69.5%) were of grade 2 and 25 cases were of grade 3(30.4% cases). There was no grade 1 tumour in our study. Considering ER, PR and Her2Neu oncoprotein overexpression and grade of tumour, twenty five cases (30.4%) were ER+ PR+ and Her2Neu negative and were all grade 2. Eleven cases (13%) were ER+PR-and Her2Neu-and were grade 2.Only one case(1.2%) was ER-PR+ and Her2Neu-and was again grade 2. Eight cases (9.7%) were tripple negative with grade 3 morphology. Twenty seven cases were ER-PR-and Her2Neu positive (32.9%). Eleven cases were ER-PR-and Her2Neu+ with grade 2 morphology and 16 cases were grade 3. Ten cases (12.1%) were tripple positive having 9 cases with grade 2 morphology and one case with grade 3 morpholgy. Conclusion: Reactivity of steroid receptors decreases with increasing grade. Tripple negative tumours usually exhibit higher grade. Her2 Neu positivity is also associated with higher grade and poor survival rate.

Epidemiological profile and distribution of prognostic factors in invasive breast cancer among Algerian women

“…Regarding the heterogeneity of breast cancer [17,18], the latest classifications established by the World Health Organization take into account the variability of the morphological, phenotypic and molecular profile of this type of cancer [6,9]. The state of hormone receptors has become a predictor of response to hormonal therapy with tamoxifen in women with hormone-dependent breast cancer [19].…”

Profile of Histopathological Type and Molecular Subtypes of Mammary Cancer of DMBA-induced Rat and its Relevancy to Human Breast Cancer

“…The previous research used a 5 mg dose of DMBA induction and 65 mg/kg BW single dose which showed that the most frequent image was grade 1, then grade 2, and the fewest was grade 3 [37], [39]. In general, in human breast cancer, the most frequent is grade 2 [40], [41]. Histopathological grading showed cell differentiation level and it had a relation with a bad prognosis of human breast cancer [42].…”

Retrospective Study Correlation Between Grade and Amount of Mitosis With Er, Pr, and Her2 Expression in Invasive Breast Carcinoma of No Special Type

“…PR expression is strongly dependent on ER expression. Tumors expressing PR but not expressing ER are very rare (<1% of breast carcinoma cases) (16). ER negative breast carcinomas increasingly do not resemble the cell of origin and tend to have more severe genetic mutations, such as p53, HER2, BRCA1 mutations and higher genomic instability (2,3).…”