Role of Hydroxyapatite and Ellagic Acid in the Osteogenesis

Wardhana, Agung Satria; Nirwana, Intan; Budi, Hendrik Setia; Surboyo, Meircurius Dwi Condro

doi:10.1055/s-0040-1714039

Cited by 18 publications

(20 citation statements)

References 32 publications

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“…Inhibition of this inflammatory process will lead to an increase in the process of tissue regeneration. In the previous study, ellagic acid is able to increase the number of osteoblast cells, the expression of OCN and OPG in the bone regeneration process (Wardhana et al, 2020). Ellagic acid has also shown that this material is not toxic to normal cells of the body, such as fibroblast (Nirwana et al, 2021).…”

Section: Discussionmentioning

confidence: 88%

“…The antiinflammatory properties works by inhibit the pro-inflammatory cytokines that affected on inhibition of bone formation or osteogenesis (Al-obaidi et al, 2014). The osteogenesis stimulation by ellagic acid involve the increased the osteocalcin (OCN), osteoprotegerin (OPG) and stimulate the osteoblast during bone regeneration (Wardhana et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The Role of Ellagic Acid on Inflammatory Protein NFKB1 and HSP70 with STITCH Prediction

Saputera¹,

Nirwana²,

Kridanto³

et al. 2022

CMUJNS

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The objective of this study was to determine the intervention mechanism of Ellagic Acid (ES) against inflammatory proteins NFKB1 and HSP70. Target Protein Analysis using SeaTarget (http://sea.bkslab.org). The compound is then analyzed for its target protein in the human body using the STITCH database (http://stitch.embl.de/). Then analyzed the interactions between proteins using the STRINGdb database (https://string-db.org/cgi/input.pl). In the analysis results with SEA, Ellagic Acid can target HSP70. Based on the results of interactions using STITCH, ellagic acid can target the Alpha Protein Kinase Catalytic Sub-unit (PRKCA). Protein Kinase Catalytic Sub-unit Alpha facilitates ellagic acid interactions with Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) and Heat Shock 60kDa Protein 1 (HSPD1). Where NFKB1 is a pleiotropic transcription factor in various processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. Ellagic acid can help reduce inflammation. It is predicted that the active compound in ellagic acid acts as an anti-inflammatory. The NFKB1 and HSP70 pathways play a role in inflammation and have activity for inflammation regulation. Keywords: Ellagic acid, HSP70, Human & Health; Inflammation, NFKB1, STITCH

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

The Role of Ellagic Acid on Inflammatory Protein NFKB1 and HSP70 with STITCH Prediction

Saputera¹,

Nirwana²,

Kridanto³

et al. 2022

CMUJNS

Self Cite

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“…31 Wardhana AS, et al use ellagic-hydroxyapatite acid to promotes osteogenesis in bone defects by increasing the amounts of osteoblasts and therefore the expression of osteoprotegerin and osteocalcin. 32 HA is a scaffold that can bond with MMPs and reduce its activities in vitro. Secretion and MMP activities are regulated well, and in normal tissues, MMP is expressed on a fundamental level.…”

Section: Discussionmentioning

confidence: 99%

Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

Damayanti

Rachmawati

2022

F1000Res

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Background: Tissue engineering technology has been used globally and proven to accelerate wound healing. This study aimed to analyse the effect of adding hydroxyapatite (HA) as a scaffold to platelet-rich fibrin (PRF) as a growth factor in accelerating the wound healing process as seen from the expression of matrix metalloproteinase-13 (MMP-13). Methods: This research is an animal experiment conducted on 18 rabbits (Oryctolagus cuniculus). Rabbits were randomly divided into the following three groups of treatment: (G1) the application of PRF group, (G2) the application of PRF+HA group and (C) the control group without any application. Furthermore, each treatment group was split randomly into three groups of observation time. Periodontal tissue biopsy was performed to analyse the histopathological features that were examined on the basis of the level of MMP-13 immunoexpression. Results: MMP-13 immunoexpression in the PRF+HA group showed better histoscore results, indicating a substantial reduction in MMP-13 values compared with other groups. The healing process was shown to increase with increasing observation time (p<0.05), and the PRF+HA group outperformed the PRF and control groups. On day 3, MMP-13 exhibited a dark brown colour of Immunohistochemistry (IHC), which indicated an increase in the expression value of MMP-13 in the early stages of healing, namely, inflammation. On day 14, light brown IHC was seen, especially in group 2, as a reference that the remodeling process had begun. Conclusions: This study indicates that the application of HA can accelerate the socket healing process by decreasing the level of immunoexpression of MMP-13. HA is an alloplastic material that has inherent bioactive properties that support osteoconduction, which functions as a scaffold in the form of a fibrin matrix that can bind MMPs so that it can accelerate the wound healing process.

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“…It has been reported that free oxygen radicals are produced in the inflammatory phase by phagocytic cells on the bone surface. In the study of Wardhana et al [ 29 ], an increase in osteoclast formation and bone resorption was observed, which is thought to prevent free radicals from remineralizating in the bone defect and tooth eruption.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Effects of Topical Ellagic Acid and Graft Application on Bone Regeneration: an Experimental Study

Gül¹,

Dündar²,

Yiğin³

et al. 2022

JOMR

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Objectives:The aim of this experimental animal study is to investigate the effect of bone graft and topical ellagic acid application on bone regeneration in rats with critical-sized calvarial bone defects. Material and Methods: A total of 24 male Wistar rats were divided into three groups, and 7 mm critical-sized calvarial bone defects were created surgically in them. In the first group, the created defect was left empty, and this acted as a control group. In the second group, only a bone graft was placed in the created defect. In the third group, in addition to placing a bone graft in the created defect, 0.325 mg/kg ellagic acid (EA) was applied topically to the defect. Results: As a result of semiquantitative scoring, osteoblast counts were 2 (SD 0.82) in the control group, 2.71 (SD 0.76) in the graft group, and 1.14 (SD 0.69) in the EA + graft group. The number of osteocytes was 2.29 (SD 0.76) in the control group, 2.71 (SD 1.11) in the graft group, and 1.43 (SD 0.54) in the EA + graft group. When inflammations were evaluated, values of 1.71 (SD 0.75), 1.14 (SD 0.69), and 3 (SD 0.82) were obtained in the control, graft, and EA + graft groups, respectively. Conclusions: Topical ellagic and graft applications show different effects at different doses under topical and systemic conditions. The dose amount of ellagic acid applied, especially in topical applications, has critical importance in bone healing.

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Role of Hydroxyapatite and Ellagic Acid in the Osteogenesis

Cited by 18 publications

References 32 publications

The Role of Ellagic Acid on Inflammatory Protein NFKB1 and HSP70 with STITCH Prediction

The Role of Ellagic Acid on Inflammatory Protein NFKB1 and HSP70 with STITCH Prediction

Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

Evaluation of the Effects of Topical Ellagic Acid and Graft Application on Bone Regeneration: an Experimental Study

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