Role of hyaluronidase inhibitors in the neutralization of toxicity of Egyptian horned viper Cerastes cerastes venom

Abstract

Hyaluronidase ''venom spreading factor'' is a common component of snake venoms and indirectly potentiates venom toxicity. It may cause permanent local tissue destruction at the bite site/systemic collapse of the envenomated victim. The present study was performed to assess the benefits of inhibiting the hyaluronidase activity of Egyptian horned viper, Cerastes cerastes (Cc). The aqueous extracts of some medicinal plants were screened for their inhibitory effect on hyaluronidase activity of Cc venom. The results revealed that the Rosmarinus officinalis (Ro) extract is the most potent hyaluronidase inhibitor among the tested extracts. The Ro extract is more potent inhibitory effect on the hyaluronidase activity than the prepared rabbit monoclonal antiserum of previously purified hyaluronidase enzyme from Cc venom (anti-CcHaseII). In addition, the Ro extract is efficiently inhibited the activity of hemorrhagic toxin previously purified from Cc venom, and it also neutralized the edema inducing activity of the Cc venom in vivo. Furthermore, the Ro extract markedly increased the survival time of experimental mice injected with lethal dose of Cc venom up to 7 h in compared to mice injected with venom alone or with venom/anti-CcHaseII (15 ± 5, 75 ± 4 min), respectively. Our findings imply the significance of plant-derived hyaluronidase inhibitor in the neutralization of local effects of Cc venom and retardation of death time. Therefore, it may use as a therapeutic value in complementary snakebite therapy.

Honey shows potent inhibitory activity against the bovine testes hyaluronidase

“…HA inhibitors have also led to new therapeutic concepts in pathophysiological conditions, such as laryngeal or breast cancer treatment, that are associated with the hyaluronanhyaluronidase system 3,4,7 . Since the partial control of hyaluronidases possesses several important physiological functions, they have recently attracted considerable scientific interest as synthetic and natural inhibitors 4,[8][9][10][11][12] . Honey and bee products are natural materials whose antioxidant, anti-inflammatory and anti-tumoral efficacies vary depending on the flora from which they are produced.…”

Rosemary leaves extract: Anti-snake action against Egyptian Cerastes cerastes venom

“…Wahby et al. 14 proved that the RMAE, a potent hyaluronidase inhibitor, inhibited the spreading property of the Cc venom.…”

“… 10 , 11 , 12 , 13 Previously, we demonstrated that the rosemary leaves aqueous extract had potent hyaluronidase inhibitory effect among several medicinal plants tested in this study. 14 From this standpoint, the present study aims to evaluate the anti-venom properties of rosemary leaf aqueous extract against Egyptian Cerastes cerastes viper venom toxicity using both in vitro and in vivo assays.…”

Caracterização de enzimas em peçonhas animais : identificação de fosfolipases do escorpião Hadrurus gerstchi e atividades enzimáticas da arraia Potamotrygon falkneri

“…Hialuronidase (TFU/mg) 4,72 x 10 5 ± 0,007 X -Reynals, 1936;Girish et al, 2004;Wahby et al, 2012b). Estudos recentes suportam a ideia da utilização de inibidores de hialuronidase ou ainda um soro específico para combater os efeitos desta enzima, em uma tentativa de diminuir os efeitos locais e sistêmicos do envenenamento (Girish e Kemparaju, 2006;Wahby et al, 2012a) A hialuronidase é uma enzima comumente encontrada na peçonha de várias espécies. Nas peçonhas de peixes já foi descrita em peixe-pedrae outras espécies de arraias (Poh et al, 1992;Magalhães et al, 2008;Madokoro et al, 2011), porém estas peçonhas, em geral, exibem uma atividade muito mais alta do que nas peçonhas de outras espécies.…”

“…Dessa forma pode se inferir que as proteases juntamente com a hialuronidase, com atividade tão alta, pode ser o principal fator atuante nos sintomas locais do envenenamento por arraias de água doce. Estudos recentes suportam a ideia da utilização de inibidores de hialuronidase ou ainda um soro específico para combater os efeitos desta enzima, em uma tentativa de diminuir os efeitos locais e sistêmicos do envenenamento (Girish e Kemparaju, 2006;Wahby et al, 2012a) (Oliveira Jr., 2014).…”