Role of HSP70 in cellular thermotolerance

Beckham, Josh T.; Wilmink, Gerald J.; Mackanos, Mark A.; Takahashi, Keiko; Contag, Christopher H.; Takahashi, Takamune; Jansen, E.

doi:10.1002/lsm.20713

Cited by 42 publications

(41 citation statements)

References 51 publications

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“…For example, cells exposed to 45°C for 15 min were observed to undergo transient G 2 block, while when exposed to 45°C for 30 min, they exhibited a long lasting G 2 phase and a late S-phase block [73]. The CSR mechanism is well documented in the literature [66][67][68][69][74][75][76][77], and the general consensus is that cells activate these mechanisms to combat and survive hyperthermic stress. CSR mechanisms involve many intracellular pathways, including redox, DNA sensing and repair, molecular chaperones, proteolysis, energy metabolism, and apoptosis [78].…”

Section: Thermal Effects On Mammalian Cellsmentioning

confidence: 98%

“…These proteins, collectively referred to as minimal stress proteins, are significantly upregulated by cells immediately after exposure to stress. The most widely studied family of minimal stress proteins are the heat shock proteins (Hsps), which includes Hsp70, Hsp40, Hsp60, and Hsp105 [66][67][68][69][74][75][76][77] (Fig. 4a, c).…”

Section: Thermal Effects On Mammalian Cellsmentioning

confidence: 99%

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Invited Review Article: Current State of Research on Biological Effects of Terahertz Radiation

Wilmink

Grundt

2011

J Infrared Milli Terahz Waves

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272

186

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In recent years, terahertz radiation sources are increasingly being exploited in military and civil applications. However, only a few studies have so far been conducted to examine the biological effects associated with terahertz radiation. In this study, we evaluated the cellular response of mesenchymal mouse stem cells exposed to THz radiation. We apply low-power radiation from both a pulsed broad-band (centered at 10 THz) source and from a CW laser (2.52 THz) source. Modeling, empirical characterization, and monitoring techniques were applied to minimize the impact of radiation-induced increases in temperature. qRT-PCR was used to evaluate changes in the transcriptional activity of selected hyperthermic genes. We found that temperature increases were minimal, and that the differential expression of the investigated heat shock proteins (HSP105, HSP90, and CPR) was unaffected, while the expression of certain other genes (Adiponectin, GLUT4, and PPARG) showed clear effects of the THz irradiation after prolonged, broad-band exposure.

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Section: Thermal Effects On Mammalian Cellsmentioning

confidence: 98%

Section: Thermal Effects On Mammalian Cellsmentioning

confidence: 99%

Invited Review Article: Current State of Research on Biological Effects of Terahertz Radiation

Wilmink

Grundt

2011

J Infrared Milli Terahz Waves

Self Cite

272

186

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“…It was found that the Hsp70 expression, as indicated by the reporter gene, contributes significantly to cellular thermotolerance. 13 However, extremely short durations, corresponding to thermal confinement times (on the order of microseconds), were not evaluated.…”

Section: Pulse Duration Determines Levels Of Hsp70 Induction In Tissumentioning

confidence: 99%

Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation

Mackanos

Contag

2011

J. Biomed. Opt.

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Abstract. Induction of heat shock protein (Hsp) expression correlates with cytoprotection, reduced tissue damage, and accelerated healing in animal models. Since Hsps are transcriptionally activated in response to stress, they can act as stress indicators in burn injury or surgical procedures that produce heat and thermal change. A fast in vivo readout for induction of Hsp transcription in tissues would allow for the study of these proteins as therapeutic effect mediators and reporters of thermal stress/damage. We used a transgenic reporter mouse in which a luciferase expression is controlled by the regulatory region of the inducible 70 kilodalton (kDa) Hsp as a rapid readout of cellular responses to laser-mediated thermal stress/injury in mouse skin. We assessed the pulse duration dependence of the Hsp70 expression after irradiation with a CO 2 laser at 10.6 μm in wavelength over a range of 1000 to 1 ms. Hsp70 induction varied with changes in laser pulse durations and radiant exposures, which defined the ranges at which thermal activation of Hsp70 can be used to protect cells from subsequent stress, and reveals the window of thermal stress that tissues can endure. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“…Increased expression of Hsp70 protects proteins from the damaging effects of stress [45,46]. This has been observed in several types of tumours, including breast and cervical cancers [33,47], and its mechanism may be involved in cell proliferation, prognosis, and drug resistance [26,48,49].…”

Section: Introductionmentioning

confidence: 96%

Measurement and mathematical modeling of thermally induced injury and heat shock protein expression kinetics in normal and cancerous prostate cells

Rylander

Feng

Zimmermann

et al. 2010

International Journal of Hyperthermia

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Role of HSP70 in cellular thermotolerance

Abstract: The data shows that HSP70 contributes significantly to cellular thermotolerance but there are other pathways that provide residual thermotolerance in cells deficient in Hsp70.

Cited by 42 publications

References 51 publications

Invited Review Article: Current State of Research on Biological Effects of Terahertz Radiation

Invited Review Article: Current State of Research on Biological Effects of Terahertz Radiation

Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation

Measurement and mathematical modeling of thermally induced injury and heat shock protein expression kinetics in normal and cancerous prostate cells

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