Measurement and mathematical modeling of thermally induced injury and heat shock protein expression kinetics in normal and cancerous prostate cells

Rylander, Marissa Nichole; Feng, Yusheng; Zimmermann, Kristen A.; Diller, Kenneth R.

doi:10.3109/02656736.2010.486778

Cited by 36 publications

(33 citation statements)

References 67 publications

(96 reference statements)

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“…These proteins are beneficial in that they are directly visualized by the fluorescent microscopy and bioimaging analyzers. In contrast, we chose the HSP70 promoter as a model promoter for gene expression, because its functions are relatively well understood in various HSP family members (14)(15)(16)(17)(18). In addition, many previous researchers have examined regulation of gene expression by the HSP70 promoter (1-8).…”

Section: Resultsmentioning

confidence: 99%

“…In particular, optical regulation of gene expression (1)(2)(3)(4)(5)(6)(7)(8) and optogenetic control of receptor and channel proteins on the cell surface (9-13) have received much research attention. Heat shock proteins (HSPs) are critical components of a cell's defense mechanism against injury associated with adverse stresses (14)(15)(16)(17)(18). Promoters that contain heat shock elements (HSEs) upstream of the HSP coding region, known as heat shock promoters, can be turned on by various stresses besides heat shock, including heavy ions, radicals, alcohol, and viral infection, suggesting that they are driven by almost all kinds of stresses (1)(2)(3)(4)(5)(6)(7)(8)(14)(15)(16)(17)(18).…”

mentioning

confidence: 99%

“…Heat shock proteins (HSPs) are critical components of a cell's defense mechanism against injury associated with adverse stresses (14)(15)(16)(17)(18). Promoters that contain heat shock elements (HSEs) upstream of the HSP coding region, known as heat shock promoters, can be turned on by various stresses besides heat shock, including heavy ions, radicals, alcohol, and viral infection, suggesting that they are driven by almost all kinds of stresses (1)(2)(3)(4)(5)(6)(7)(8)(14)(15)(16)(17)(18). Given the simple and highly controllable nature of heat shock treatment, the heat shock response is ubiquitous in all organisms from bacteria to higher vertebrates.…”

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confidence: 99%

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Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Miyako

Deguchi

Nakajima

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The development of optical methods to control cellular functions is important for various biological applications. In particular, heat shock promoter-mediated gene expression systems by laser light are attractive targets for controlling cellular functions. However, previous approaches have considerable technical limitations related to their use of UV, short-wavelength visible (vis), and infrared (IR) laser light, which have poor penetration into biological tissue. Biological tissue is relatively transparent to light inside the diagnostic window at wavelengths of 650–1,100 nm. Here we present a unique optical biotechnological method using carbon nanohorn (CNH) that transforms energy from diagnostic window laser light to heat to control the expression of various genes. We report that with this method, laser irradiation within the diagnostic window resulted in effective heat generation and thus caused heat shock promoter-mediated gene expression. This study provides an important step forward in the development of light-manipulated gene expression technologies.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Miyako

Deguchi

Nakajima

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…15,16 In terms of the role of HSPs in bone repair, HSP27 and HSP70 have been shown to be highly expressed in osteoblasts of new bone-generating areas, along with type I collagen, as well as in rat tibia and in preosteoblasts after identical heating conditions in vitro. 32,34,35 HSP27, in particular, plays an important role in bone regeneration through the up-regulation of TGF-b, 36 estrogen, 37 endothelin-1, 38 and prostaglandins. 39 In summary, this indicates that HSPs are crucial for tissue regeneration.…”

Section: Et Almentioning

confidence: 99%

Mild Heat Stress Enhances Angiogenesis in a Co-culture System Consisting of Primary Human Osteoblasts and Outgrowth Endothelial Cells

Fuchs²,

Böse³

et al. 2014

Tissue Engineering Part C: Methods

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The repair and regeneration of large bone defects, including the formation of functional vasculature, represents a highly challenging task for tissue engineering and regenerative medicine. Recent studies have shown that vascularization and ossification can be stimulated by mild heat stress (MHS), which would offer the option to enhance the bone regeneration process by relatively simple means. However, the mechanisms of MHS-enhanced angiogenesis and osteogenesis, as well as potential risks for the treated cells are unclear. We have investigated the direct effect of MHS on angiogenesis and osteogenesis in a co-culture system of human outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs), and assessed cytotoxic effects, as well as the levels of various heat shock proteins (HSPs) synthesized under these conditions. Enhanced formation of microvessel-like structures was observed in co-cultures exposed to MHS (41°C, 1 h), twice per week, over a time period of 7-14 days. As shown by real-time polymerase chain reaction (PCR), the expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), and tumor necrosis factor-alpha was up-regulated in MHS-treated co-cultures 24 h post-treatment. At the protein level, significantly elevated VEGF and Ang-1 concentrations were observed in MHS-treated co-cultures and pOB mono-cultures compared with controls, indicating paracrine effects associated with MHS-induced angiogenesis. MHS-stimulated co-cultures and OEC mono-cultures released higher levels of Ang-2 than untreated cultures. On the other hand MHS treatment of cocultures did not result in a clear effect regarding osteogenesis. Nevertheless, real-time PCR demonstrated that MHS increased the expression of mitogen-activated protein kinase, interleukin-6, and bone morphogenetic protein 2, known as HSP-related molecules in angiogenic and osteogenic regulation pathways. In agreement with these observations, the expression of some selected HSPs also increased at both the mRNA and protein levels in MHS-treated co-cultures.

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“…Longer treatment time is needed for lower temperature, or vice versa. For example, cell necrosis requires 135 min at 43˚C, but only 5 sec at 57˚C [34,35]. Different temperature is needed depending on the treatment protocol or treatment objective.…”

Section: Introductionmentioning

confidence: 99%

Photothermal Effect of PEG-Functionalized Single-Walled Carbon Nanotubes

Zeng¹

2017

NTMB

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Measurement and mathematical modeling of thermally induced injury and heat shock protein expression kinetics in normal and cancerous prostate cells

Cited by 36 publications

References 67 publications

Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Mild Heat Stress Enhances Angiogenesis in a Co-culture System Consisting of Primary Human Osteoblasts and Outgrowth Endothelial Cells

Photothermal Effect of PEG-Functionalized Single-Walled Carbon Nanotubes

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