Role of HD2 genes in seed germination and early seedling growth in Arabidopsis

Colville, Adam; Alhattab, Reem; Hu, Ming; Labbé, Hélène; Xing, Tim; Miki, Brian

doi:10.1007/s00299-011-1105-z

Cited by 56 publications

(49 citation statements)

References 54 publications

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“…In conclusion, APO and AMPO can inhibit different aspects of plant growth at concentrations comparable to the effective concentration of commercial herbicides. The root growth defects were comparable to those seen in plants lacking function of HDA6 and HDA19 (RPD3/HDA1 superfamily; Supplemental Figure 9) and of HDT1 and HDT3 (plant-specific HD2 family) (Colville et al, 2011), supporting the hypothesis that this class of allelochemicals affects the germination and development of surrounding competitors by inhibiting one or several HDACs.…”

Section: Similar Phytotoxicity Of Hdac Inhibitors and Conventional Hesupporting

confidence: 54%

“…Of note, the plant-derived aminophenoxazinone APO showed the clearest correlation between effects on H3ac and gene expression in Arabidopsis, in agreement with APO effects being physiologically most relevant. Characterized mutants in single HDAC genes show less severe slowdown of root growth than plants treated with high concentrations of APO (Supplemental Figure 9; Colville et al, 2011), which suggests that the allelochemical either acts on other HDACs or that it inhibits more than one HDAC. Elucidating the specificity of the allelochemicals APO and AMPO in inhibiting different plant HDACs, and the consequences for different histones and for modifications at different histone residues, should therefore allow a better understanding of their direct chromatin targets.…”

Section: Discussionmentioning

confidence: 99%

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Plants Release Precursors of Histone Deacetylase Inhibitors to Suppress Growth of Competitors

et al. 2015

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To secure their access to water, light, and nutrients, many plant species have developed allelopathic strategies to suppress competitors. To this end, they release into the rhizosphere phytotoxic substances that inhibit the germination and growth of neighbors. Despite the importance of allelopathy in shaping natural plant communities and for agricultural production, the underlying molecular mechanisms are largely unknown. Here, we report that allelochemicals derived from the common class of cyclic hydroxamic acid root exudates directly affect the chromatin-modifying machinery in Arabidopsis thaliana. These allelochemicals inhibit histone deacetylases both in vitro and in vivo and exert their activity through locus-specific alterations of histone acetylation and associated gene expression. Our multilevel analysis collectively shows how plant-plant interactions interfere with a fundamental cellular process, histone acetylation, by targeting an evolutionarily highly conserved class of enzymes.

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Section: Similar Phytotoxicity Of Hdac Inhibitors and Conventional Hesupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Plants Release Precursors of Histone Deacetylase Inhibitors to Suppress Growth of Competitors

et al. 2015

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“…Histone acetylation has been demonstrated to function in different plant hormones (23,(26)(27)(28)(29), and several histone deacetylases are known to be involved in the regulation of gene expression (30)(31)(32)(33)(34)(35)(36)(37). The data presented here showed that the C-terminal domain of EIN2 (EIN2-C) is the key factor that drives alterations in histone acetylation during the ethylene response.…”

Section: Discussionmentioning

confidence: 64%

EIN2 mediates direct regulation of histone acetylation in the ethylene response

Zhang

Wang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

131

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Ethylene gas is essential for developmental processes and stress responses in plants. Although the membrane-bound protein EIN2 is critical for ethylene signaling, the mechanism by which the ethylene signal is transduced remains largely unknown. Here we show the levels of H3K14Ac and H3K23Ac are correlated with the levels of EIN2 protein and demonstrate EIN2 C terminus (EIN2-C) is sufficient to rescue the levels of H3K14/23Ac of ein2-5 at the target loci, using CRISPR/dCas9-EIN2-C. Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) and ChIP-reChIP-seq analyses revealed that EIN2-C associates with histone partially through an interaction with EIN2 nuclear-associated protein1 (ENAP1), which preferentially binds to the genome regions that are associated with actively expressed genes both with and without ethylene treatments. Specifically, in the presence of ethylene, ENAP1-binding regions are more accessible upon the interaction with EIN2, and more EIN3 proteins bind to the loci where ENAP1 is enriched for a quick response. Together, these results reveal EIN2-C is the key factor regulating H3K14Ac and H3K23Ac in response to ethylene and uncover a unique mechanism by which ENAP1 interacts with chromatin, potentially preserving the open chromatin regions in the absence of ethylene; in the presence of ethylene, EIN2 interacts with ENAP1, elevating the levels of H3K14Ac and H3K23Ac, promoting more EIN3 binding to the targets shared with ENAP1 and resulting in a rapid transcriptional regulation.T he plant hormone ethylene is essential for a myriad of physiological and developmental processes. It is important in responses to stress, such as drought, cold, flooding, and pathogen infection (1, 2), and modulates stem cell division (3). The common aquatic ancestor of plants possessed the ethylene signaling pathway and the mechanism has been elucidated by analysis of Arabidopsis (4). Ethylene is perceived by a family of receptors bound to the membrane of the endoplasmic reticulum (ER) that are similar in sequence and structure to bacterial two-component histidine kinases (5-9). Each ethylene receptor has an N-terminal transmembrane domain, and the receptors form dimers that bind ethylene via a copper cofactor RAN1 (10, 11). Signaling from one of the receptors, ETR1, induces its physical association with the ER-localized protein RTE1 (12). The ethylene receptors function redundantly to negatively regulate ethylene responses (5) via a downstream Raf-like protein kinase called CTR1 (13,14).In the absence of ethylene, both the ethylene receptors and CTR1 are active, and CTR1 is associated with the ER membrane through a direct interaction with ETR1 (13). The CTR1 downstream factor, EIN2, is localized to the ER membrane, where it interacts with ETR1 (15). The protein stability of EIN2 is regulated by two F-box proteins, ETP1 and ETP2, which mediated its degradation by the ubiquitin-proteasome pathway (16). In the absence of ethylene, the CTR1-mediated phosphorylation at the C-terminal end of EIN2 (EIN2-C) leads to ...

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“…They found that AtHD2A prevents germination while AtHD2C enhances germination in the presence of Glc. In contrast to AtHD2A and AtHD2C, AtHDA7 is not induced by Glc (Colville et al, 2011), thereby making it unlikely that sugar sensing is involved in the seed germination defects observed in Athda7-2. The similarity between the Athda7-2 phenotype and TSA treatment (Tanaka et al, 2008) suggests a role for AtHDA7 in Arabidopsis seed germination.…”

Section: Discussionmentioning

confidence: 97%

Histone Deacetylase AtHDA7 Is Required for Female Gametophyte and Embryo Development in Arabidopsis

et al. 2013

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Histone modifications are involved in the regulation of many processes in eukaryotic development. In this work, we provide evidence that AtHDA7, a HISTONE DEACETYLASE (HDAC) of the Reduced Potassium Dependency3 (RPD3) superfamily, is crucial for female gametophyte development and embryogenesis in Arabidopsis (Arabidopsis thaliana). Silencing of AtHDA7 causes degeneration of micropylar nuclei at the stage of four-nucleate embryo sac and delay in the progression of embryo development, thereby bringing the seed set down in the Athda7-2 mutant. Furthermore, AtHDA7 down-and up-regulation lead to a delay of growth in postgermination and later developmental stages. The Athda7-2 mutation that induces histone hyperacetylation significantly increases the transcription of other HDACs (AtHDA6 and AtHDA9). Moreover, silencing of AtHDA7 affects the expression of ARABIDOPSIS HOMOLOG OF SEPARASE (AtAESP), previously demonstrated to be involved in female gametophyte and embryo development. However, chromatin immunoprecipitation analysis with acetylated H3 antibody provided evidence that the acetylation levels of H3 at AtAESP and HDACs does not change in the mutant. Further investigations are essential to ascertain the mechanism by which AtHDA7 affects female gametophyte and embryo development.

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Role of HD2 genes in seed germination and early seedling growth in Arabidopsis

Cited by 56 publications

References 54 publications

Plants Release Precursors of Histone Deacetylase Inhibitors to Suppress Growth of Competitors

Plants Release Precursors of Histone Deacetylase Inhibitors to Suppress Growth of Competitors

EIN2 mediates direct regulation of histone acetylation in the ethylene response

Histone Deacetylase AtHDA7 Is Required for Female Gametophyte and Embryo Development in Arabidopsis

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