Histone Deacetylase AtHDA7 Is Required for Female Gametophyte and Embryo Development in Arabidopsis

Cigliano, Riccardo Aiese; Cremona, Gaetana; Paparo, Rosa; Termolino, Pasquale; Perrella, Giorgio; Gutzat, Ruben; Consiglio, Federica; Conicella, Clara

doi:10.1104/pp.113.221713

Cited by 60 publications

(48 citation statements)

References 51 publications

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“…Recently, a role for HISTONE DEACETYLASE7 (HDA7) during megagametogenesis and embryo development has been demonstrated (Cigliano et al, 2013). In hda7-2 mutants at the four-nucleate stage of megagametogenesis, the two nuclei located at the micropylar pole degenerate, suggesting that histone deacetylation is required for survival and possibly for fate determination of the micropylar nuclei (Table 3; Cigliano et al, 2013). Other important gene regulatory control mechanisms involve the storage of mRNAs in mRNA-protein complexes, mRNA processing and mRNA degradation (reviewed by Hafidh et al, 2011).…”

Section: Epigenetic and Post-transcriptional Regulation Of Gametophytmentioning

confidence: 99%

Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction

2015

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The life cycle of flowering plants alternates between two heteromorphic generations: a diploid sporophytic generation and a haploid gametophytic generation. During the development of the plant reproductive lineages -the germlines -typically, single sporophytic (somatic) cells in the flower become committed to undergo meiosis. The resulting spores subsequently develop into highly polarized and differentiated haploid gametophytes that harbour the gametes. Recent studies have provided insights into the genetic basis and regulatory programs underlying cell specification and the acquisition of reproductive fate during both sexual reproduction and asexual (apomictic) reproduction. As we review here, these recent advances emphasize the importance of transcriptional, translational and post-transcriptional regulation, and the role of epigenetic regulatory pathways and hormonal activity.

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Section: Epigenetic and Post-transcriptional Regulation Of Gametophytmentioning

confidence: 99%

Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction

2015

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“…The 18 HDACs identified in Arabidopsis (2) can be categorized into three groups based on phylogenetic analysis: reduced potassium dependency-3/histone deacetylase-1 (RPD3/HDA1), histone deacetylase-2 (HD2), and silent information regulator-2 (SIR2)-like (3). Twelve HDACs belong to the RPD3/HDA1 group (3) and are involved in various biological processes, such as organ development, reproductive processes, hormone signaling, and DNA methylation (4)(5)(6)(7)(8)(9). They can be further classified into three classes based on sequence homology (3).…”

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confidence: 99%

POWERDRESS and HDA9 interact and promote histone H3 deacetylation at specific genomic sites in Arabidopsis

Kim

Wang

Gao

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

129

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Histone acetylation is a major epigenetic control mechanism that is tightly linked to the promotion of gene expression. Histone acetylation levels are balanced through the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). Arabidopsis HDAC genes (AtHDACs) compose a large gene family, and distinct phenotypes among AtHDAC mutants reflect the functional specificity of individual AtHDACs. However, the mechanisms underlying this functional diversity are largely unknown. Here, we show that POWERDRESS (PWR), a SANT (SWI3/DAD2/N-CoR/TFIII-B) domain protein, interacts with HDA9 and promotes histone H3 deacetylation, possibly by facilitating HDA9 function at target regions. The developmental phenotypes of pwr and hda9 mutants were highly similar. Three lysine residues (K9, K14, and K27) of H3 retained hyperacetylation status in both pwr and hda9 mutants. Genome-wide H3K9 and H3K14 acetylation profiling revealed elevated acetylation at largely overlapping sets of target genes in the two mutants. Highly similar gene-expression profiles in the two mutants correlated with the histone H3 acetylation status in the pwr and hda9 mutants. In addition, PWR and HDA9 modulated flowering time by repressing AGAMOUS-LIKE 19 expression through histone H3 deacetylation in the same genetic pathway. Finally, PWR was shown to physically interact with HDA9, and its SANT2 domain, which is homologous to that of subunits in animal HDAC complexes, showed specific binding affinity to acetylated histone H3. We therefore propose that PWR acts as a subunit in a complex with HDA9 to result in lysine deacetylation of histone H3 at specific genomic targets.SANT domain | POWERDRESS | HDA9 | histone deacetylation | AGL19 P osttranslational modifications of histones-including acetylation, methylation, phosphorylation, and ubiquitinationplay important roles in plant development, genome integrity, and stress responses. Histone acetylation/deacetylation, a reversible process, promotes/represses gene expression (1) and occurs at lysine residues within histone N-terminal tails. The histone acetylation status is regulated by counteracting enzymes: histone acetyltransferases (HATs) and histone deacetylases (HDACs). The 18 HDACs identified in Arabidopsis (2) can be categorized into three groups based on phylogenetic analysis: reduced potassium dependency-3/histone deacetylase-1 (RPD3/HDA1), histone deacetylase-2 (HD2), and silent information regulator-2 (SIR2)-like (3). Twelve HDACs belong to the RPD3/HDA1 group (3) and are involved in various biological processes, such as organ development, reproductive processes, hormone signaling, and DNA methylation (4-9). They can be further classified into three classes based on sequence homology (3). The HD2 group is plant-specific and includes four HDACs that act in plant development and stress responses (10-13). The two HDACs encoded by the SIR2 family genes in Arabidopsis, SRT1 and SRT2, regulate mitochondrial energy metabolism and cellular dedifferentiation, respectively (14,15).I...

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“…In contrast to the decreased expression in gcn5 mutants, FRD3 expression increased in hda7 and hda14 mutants. It has been reported that GCN5 and HDA7 acted on the same sites of H3K9 and H3K14 but with opposite effects (Imoberdorf et al, 2006;Jin et al, 2011;Cigliano et al, 2013). Remarkably, the H3K9ac levels at the FRD3 locus were up-regulated in hda7 mutants, indicating that HDA7 might antagonistically act with GCN5 to regulate FRD3 expression.…”

Section: Complex Mechanisms Underlie the Regulation Of Iron Homeostasmentioning

confidence: 69%

“…9A). Because HDA7 mainly catalyzes deacetylation in Lys-9 and Lys-14 sites of histone 3 in Arabidopsis (Cigliano et al, 2013), we designed five pairs of primers spanning the core promoter and gene body regions and examined the H3K9/14ac modifications at the FRD3 locus by performing ChIP-PCR experiments (Fig. 9B).…”

Section: Hdacs Negatively Regulate Frd3 Expressionmentioning

confidence: 99%

GENERAL CONTROL NONREPRESSED PROTEIN5-Mediated Histone Acetylation of FERRIC REDUCTASE DEFECTIVE3 Contributes to Iron Homeostasis in Arabidopsis

et al. 2015

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Iron homeostasis is essential for plant growth and development. Here, we report that a mutation in GENERAL CONTROL NONREPRESSED PROTEIN5 (GCN5) impaired iron translocation from the root to the shoot in Arabidopsis (Arabidopsis thaliana). Illumina high-throughput sequencing revealed 879 GCN5-regulated candidate genes potentially involved in iron homeostasis. Chromatin immunoprecipitation assays indicated that five genes (At3G08040, At2G01530, At2G39380, At2G47160, and At4G05200) are direct targets of GCN5 in iron homeostasis regulation. Notably, GCN5-mediated acetylation of histone 3 lysine 9 and histone 3 lysine 14 of FERRIC REDUCTASE DEFECTIVE3 (FRD3) determined the dynamic expression of FRD3. Consistent with the function of FRD3 as a citrate efflux protein, the iron retention defect in gcn5 was rescued and fertility was partly restored by overexpressing FRD3. Moreover, iron retention in gcn5 roots was significantly reduced by the exogenous application of citrate. Collectively, these data suggest that GCN5 plays a critical role in FRD3-mediated iron homeostasis. Our results provide novel insight into the chromatinbased regulation of iron homeostasis in Arabidopsis.

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Histone Deacetylase AtHDA7 Is Required for Female Gametophyte and Embryo Development in Arabidopsis

Cited by 60 publications

References 51 publications

Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction

Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction

POWERDRESS and HDA9 interact and promote histone H3 deacetylation at specific genomic sites in Arabidopsis

GENERAL CONTROL NONREPRESSED PROTEIN5-Mediated Histone Acetylation of FERRIC REDUCTASE DEFECTIVE3 Contributes to Iron Homeostasis in Arabidopsis

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