Role of granulosa and theca cells on in vitro progesterone production in preovulatory follicles of the Japanese quail.

Mori, Masataka; Kohmoto, Kaoru; Shoda, Yoichi

doi:10.2141/jpsa.21.206

Cited by 20 publications

(3 citation statements)

References 17 publications

(2 reference statements)

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“…Progesterone concentrations in the medium removed at 66 h of culture and in the medium during the additional 3 h of incubation were measured by validated radioimmunoassay (Mori et al, 1984). The overall intra-assay and interassay coefficients of variation were 10·4% and 12·5%, respectively, and the lower limit of assay sensitivity was 5 pg/tube.…”

Section: Methodsmentioning

confidence: 99%

Effects of oestradiol-17 beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

Sasanami¹,

Mori²

1999

British Poultry Science

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In order to study the effects of steroid hormones on steroidogenesis in the avian ovary, quail granulosa cells were cultured with follicle stimulating hormone (FSH), oestradiol-17beta or testosterone. The progesterone content of the medium during the culture period of 66 h and the following 3 h of incubation with luteinising hormone (LH), was measured by radioimmunoassay. When FSH, oestradiol-17beta or testosterone were added during the 66 h culture, subsequent progesterone production by the cells during 3 h of incubation with LH was significantly increased. However, testosterone also stimulated progesterone production in the medium during the 66 h culture period, whereas FSH oroestradiol-17beta did not. Addition of staurosporine during culture inhibited both LH-stimulated progesterone production and testosterone-stimulated progesterone production. These results indicate that the processes during which granulosa cells acquired responsiveness to LH, and testosterone stimulates progesterone production might both be mediated by a staurosporine-sensitive protein kinase C-dependent pathway in quail granulosa cells.

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Section: Methodsmentioning

confidence: 99%

Effects of oestradiol-17 beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

Sasanami¹,

Mori²

1999

British Poultry Science

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“…In the smaller pre-ovulatory follicles, the second (F2) and the third (F3) largest follicles, P4 is rapidly metabolised due to their significant higher amounts of conversion enzymes in the theca cells (Huang & Nalbandov 1979, Mori et al 1984, 1985, Mori 1987.…”

Section: Progesterone (P4) Assaymentioning

confidence: 99%

Contribution of progesterone, follicle stimulating hormone and glucocorticoids in survival of serum-free cultured granulosa cell explants

Mussche

D’Herde²

2001

Journal of Endocrinology

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To investigate the role of progesterone (P4) as a survival factor in quail granulosa cell explants, P4 content was determined under various conditions and correlated with apoptotic indexes (AIs) evaluated by 2 ,6 -diamidino-2-phenylindole (DAPI)-staining. Analysis of serum-free cultures from 24 to 96 h shows decreased P4 levels in the medium paralleled by increasing AI. Inhibiting apoptosis by gonadotropic support (FSH, 100 ng/ml) stimulates a 3-fold increase of the P4 level in the medium (83·49 8·69 vs 26·31 1·61 ng/ml in serum-free controls) together with a significant decrease in AI from 8·81 1·06% in serum-free controls to 3·50 0·72%. Substantial evidence for P4 as an autocrine/paracrine survival factor can be inferred from experiments with aminoglutethimide (AG, 1 mM) and RU486 (20 µM). Blocking P4 synthesis by AG causes a 2-fold increase in apoptosis from 6·08 0·67% in serum-free controls to 12·53 1·60%. Blocking P4 receptors by RU486 causes a similar increase in AI (3·02 0·98% in serum-free controls to 17·07 3·20%) and about a 50% decrease in P4. The effect of RU486 could be attenuated by exogenous P4 but not by dexamethasone indicating selective binding of P4 to the progesterone receptor. Dexamethasone treatment promotes survival without affecting P4 levels. In further support of an autocrine/paracrine action for P4 in the granulosa cells, both the A and B form of the avian P4 receptor (PR) are identified in vivo and in vitro by Western blotting. Exogenous administration of P4 only affects survival when endogenous P4 synthesis is blocked or after 48 h of serum-free culture when endogenous P4 production is very low. Because FSH also affects survival when its stimulatory effect on P4 synthesis is blocked by AG (AI decrease from 6·08 0·67% in serum-free controls to 1·64 0·71% in FSH+AG treated) it is proposed that (1) P4 is an autocrine/paracrine survival factor in the preovulatory granulosa and (2) FSH mediates both P4-dependent and P4-independent survival pathways.

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“…,1964;OPEL,1966). Granulosa layer isolated from the largest preovulatory follicle as described previously (GILSERT et al, 1977;MORI et al, 1984) Most of our understanding of polarity of cells has come from studies using bicameral chamber system in which the cells reach confluency after culture for several days (CARSON et al, 1988;GLASSER et al, 1988). Apically directed secretion was observed for inhibin by rat Sertoli cells (HANDELSMAN et al, 1989), and for various proteins and proteoglycans by rat uterine epithelial cells (JULIAN et al, 1992), while transferrin was secreted basally by rat Sertoli cells (ONODA et al, 1990).…”

mentioning

confidence: 97%

Polarized Secretion of Progesterone by Granulosa Cells of the Japanese Quail(Coturnix coturnix japonica).

Mori

Sudo

1993

Jpn. poult. sci.

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Role of granulosa and theca cells on in vitro progesterone production in preovulatory follicles of the Japanese quail.

Cited by 20 publications

References 17 publications

Effects of oestradiol-17 beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

Effects of oestradiol-17 beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

Contribution of progesterone, follicle stimulating hormone and glucocorticoids in survival of serum-free cultured granulosa cell explants

Polarized Secretion of Progesterone by Granulosa Cells of the Japanese Quail(Coturnix coturnix japonica).

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