Effects of oestradiol-17 beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

Sasanami, Tomohiro; Mori, Masataka

doi:10.1080/00071669987322

Cited by 19 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, it has been demonstrated that culture of hen granulosa cells with oestradiol significantly increased progesterone production in response to LH (Kamiyoshi et al, Acta Veterinaria Hungarica 57, 2009 1992; Caicedo et al, 1997). Similar effects were observed in respect to granulosa cells of Japanese quails by Sasanami and Mori (1999). It cannot be excluded that the decrease in progesterone concentration in ovarian follicles of TAM-treated hens is a result of regression of the preovulatory follicles, which was also observed in earlier studies (Sechman et al, 2004b;Proszkowiec-Węglarz et al, 2005).…”

Section: (A) (B)supporting

confidence: 63%

“…They stimulate the growth of the oviduct, induce synthesis of several egg proteins in the oviduct and the liver, and are involved in egg shell formation (Sakimura et al, 2001;Dougherty and Sanders, 2005). Moreover, oestrogens influence P4 production by granulosa cells of the hen ovarian follicles Caicedo et al, 1997;Sasanami and Mori, 1999). The effects of these steroids in the target cells are mediated by oestrogen receptors (ERs) which are expressed not only in the ovary and oviduct but also in several other tissues, including the hypothalamus and pituitary gland (Gasc and Baulieu, 1988;Hrabia et al, 2004).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of tamoxifen on sex steroid concentrations in chicken ovarian follicles

Rzasa

Sechman

Paczoska-Eliasiewicz

et al. 2009

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

The aim of the present investigation was to study the effect of tamoxifen (TAM), an oestrogen receptor antagonist, on the concentrations of sex hormones in chicken ovarian follicles. The experiment was carried out on Hy-line hens which were randomly divided into two groups (control and experimental). TAM was given at a dose of 4 mg/hen (per os) at first once a day for 7 consecutive days, and subsequently four times a day for the next 6 days. Control hens received placebo. Birds were killed on the day after the last TAM treatment. From the dissected ovaries the following compartments were isolated: stroma with follicles < 1 mm, white non-hierarchical (1-4 mm and 4-8 mm) and yellow hierarchical follicles (F6-F1; 18-35 mm). The concentrations of the sex steroids progesterone (P4), testosterone (T) and oestradiol (E2) in the ovarian follicles were determined by radioimmunoassay. In the TAM-treated group, a gradual decrease in egglaying rate was observed from the 4th day of the experiment. Eventually, egg laying stopped entirely on the 12th day of the experiment. TAM significantly decreased the weight of the ovary and affected the sex hormone concentrations in the ovarian follicles. Following TAM treatment (1) a significant increase in E2 and T concentrations in the stroma, white follicles and the F4 and F1 follicles, (2) a significant decrease in E2 and T concentrations in the F2 follicle, and (3) a significant decline of P4 in the F4 to F1 follicles were observed. The results indicate that the blockade of oestrogen receptors by TAM significantly modulates the process of chicken ovarian steroidogenesis.

show abstract

Section: (A) (B)supporting

confidence: 63%

mentioning

confidence: 99%

Effect of tamoxifen on sex steroid concentrations in chicken ovarian follicles

Rzasa

Sechman

Paczoska-Eliasiewicz

et al. 2009

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

show abstract

“…This observation is consistent with the presence of androgen receptors in these cells (Yoshimura et al 1993). Our finding confirmed that of Sasanami & Mori (1999) who demonstrated that 1 mg/ml of testosterone stimulated progesterone output by cultured quail granulosa cells and increases their sensitivity to LH. Taken together, these observations suggest that the pre-ovulatory release of testosterone may facilitate the pre-ovulatory release of progesterone from granulosa cells in the F1 pre-ovulatory follicle.…”

Section: Introductionsupporting

confidence: 81%

Testosterone stimulates progesterone production and STAR, P450 cholesterol side-chain cleavage and LH receptor mRNAs expression in hen (Gallus domesticus) granulosa cells

Rangel¹,

Rodríguez²,

Rojas³

et al. 2009

Reproduction

View full text Add to dashboard Cite

The chicken ovary is organized into a hierarchy of yellow yolky follicles that ovulate on successive days. Active or passive immunization of laying hens against testosterone blocks ovulation without affecting follicle development. Testosterone may play a role in pre-ovulatory follicle maturation by stimulating granulosa progesterone production. We assessed whether this stimulus is dose-related and depends on the maturity of the donor follicle, and if it does so by stimulating granulosa cell STAR, P450 cholesterol side-chain cleavage (P450scc), and LH receptor (LHCGR) mRNAs expression. Progesterone production by granulosa cells from F1, F3, and F4 follicles, cultured for 3 h without testosterone was greater in cells collected 11-14 h than 1-4 h after ovulation. These differences in progesterone production were less pronounced after granulosa cells had been cultured for 24 h. Culture of granulosa cells for 3 or 24 h with testosterone (1-100 ng/ml) stimulated progesterone production in cells collected from F4, F3, or F1 follicles 1-4, or 11-14 h after ovulation. Testosterone (0-4000 ng/ml) alone or in combination with LH (0-100 ng/ml) increased progesterone production by F1 granulosa cells, collected 1-4 and 11-14 h after ovulation and cultured for 3 h. Finally, testosterone (10 or 100 ng/ml) increased STAR, P450scc, and LHCGR mRNAs, when added to 3 h cultures of F1 granulosa cells. In conclusion, testosterone stimulates granulosa cell progesterone production in hen pre-ovulatory hierarchical follicles irrespective of maturational state, acting alone or additively with LH. We propose that testosterone promotes granulosa cell maturation to facilitate the pre-ovulatory release of LH.

show abstract

“…Evidence from both mammalian and avian models suggests that the effects of LH are partially mediated by active PKC signaling (Morris & Richards 1993, 1995, Jamaluddin et al 1994, Sasanami & Mori 1999.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the well-documented role of cAMP in LH signaling, accumulating evidence from both mammalian and avian granulosa cells suggests that the actions of LH are, at least in part, dependent upon protein kinase C (PKC) activity. Studies incorporating the use of PKC inhibitors indicate that PKC is required for LH-induced progesterone synthesis in rat, hen, and quail preovulatory follicles (Jamaluddin et al 1994, Morris & Richards 1995, Sasanami & Mori 1999. Moreover, in the rat, phorbol ester activation of the PKC pathway promotes luteinization in the presence of subovulatory doses of LH, indicating a supportive role for PKC in final differentiation.…”

Section: Introductionmentioning

confidence: 99%

Protein kinase C activity mediates LH-induced ErbB/Erk signaling in differentiated hen granulosa cells

Woods

Johnson

2007

Reproduction

View full text Add to dashboard Cite

While there is accumulating evidence that mitogen-activated protein kinase/Erk and protein kinase C (PKC) signaling inhibits premature differentiation of granulosa cells in hen prehierarchal follicles, it has only recently been established that these signaling pathways play an important facilitory role in promoting steroidogenesis in differentiated granulosa cells from preovulatory follicles. The present studies were conducted with differentiated granulosa cells to establish the ability of LH to initiate PKC activity, and the subsequent requirement for PKC activity in promoting the ErbB/Erk signaling cascade that ultimately facilitates LH-induced progesterone production. Incubation of differentiated granulosa cells with LH increases PKC activity within 15 min, and latently promotes Erk phosphorylation (P-Erk) by 180 min. Inhibition of PKC activity with GF109203X attenuates LH-and 8-bromo-cAMP (8-br-cAMP)-induced P-Erk, but not P-Erk promoted by an epidermal growth factor (EGF) family ligand (e.g., transforming growth factor a). Importantly, inhibition of PKC activity also blocks the LH-induced increase in the autocrine expression of mRNA encoding the EGF family ligands, such as EGF, amphiregulin, and betacellulin. Furthermore, inhibition of EGF ligand shedding at the level of the cell membrane using the matrix metalloprotease activity inhibitor, GM6001, prevents both LH-and 8-br-cAMP-induced P-Erk and progesterone production. These findings provide evidence for a facilitory role of PKC and ErbB/Erk signaling in LH-induced progesterone production, place the requirement for PKC activation upstream of ErbB/Erk activity, and demonstrate for the first time in a non-mammalian vertebrate the requirement for PKC activity in LH-induced expression of EGF family ligands in granulosa cells.

show abstract

Effects of oestradiol-17 beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

Cited by 19 publications

References 27 publications

Effect of tamoxifen on sex steroid concentrations in chicken ovarian follicles

Effect of tamoxifen on sex steroid concentrations in chicken ovarian follicles

Testosterone stimulates progesterone production and STAR, P450 cholesterol side-chain cleavage and LH receptor mRNAs expression in hen (Gallus domesticus) granulosa cells

Protein kinase C activity mediates LH-induced ErbB/Erk signaling in differentiated hen granulosa cells

Contact Info

Product

Resources

About