Role of glyoxalase I in the proliferation and apoptosis control of human LNCaP and PC3 prostate cancer cells

Abstract

Our data represent the first systematic demonstration that GLOI cannot be considered a general marker of cell proliferation and that acts as a pro-survival factor in invasive PC3 cells by elusing apoptosis. GLOI may be involved in prostate cancer progression, via the control of key molecules in the mitochondrial apoptotic mechanism, through NF-kB signaling pathway.

Glyoxalase I inhibition induces apoptosis in irradiated MCF-7 cells via a novel mechanism involving Hsp27, p53 and NF-κB

“…Apoptosis was detected by evaluating DNA fragmentation by TdT-mediated dUTP nick-end labelling (TUNEL) assay (ApoAlert DNA Fragmentation Assay, Clontech Laboratories, Inc., Santa Clara, CA, USA) and agarose gel electrophoresis (Antognelli et al , 2013). …”

Andrographolide induces apoptosis via down-regulation of glyoxalase 1 and HMG-CoA reductase in HL-60 cells

“…Recently, Andro has been reported to induce apoptosis in nude mice with xenografted Hep3B tumors through the regulation of cellular GSH homeostasis (Ji, Shen, Jiang, Morahan, & Wang, 2011). A novel mechanism of MG cytotoxicity in prostate cancer cells has also been proposed (Antognelli, Mezzasoma, Fettucciari, Mearini, & Talesa, 2013). The authors silenced GLO1, and found that the resulting excess MG was cytotoxic on prostate LNCaP and PC3 cancer cells and that such cytotoxicity occurred via the key cell survival NF-κB signaling pathway.…”

Glyoxalase I in Tumor Cell Proliferation and Survival and as a Potential Target for Anticancer Therapy

“…Hyperactivation of AP-2α, E2F4 and Nrf2 has been identified in human tumors, which induces GLO1 overexpression [29,30]. The high expression of GLO1 enhances transcriptional activities of NF-κB and AP-1, and thus activates PI3K/Akt signaling for cell survival and proliferation [20,23]. Moreover, abnormal expression of GLO1 accelerates methylglyoxal metabolism, which in turn inhibits cell apoptosis.…”

“…Since a positive correlation between the level of GLO1 in prostate cancer tissues and the rate of cell proliferation was found, we considered that GLO1 may represent a risk factor for the development and progression of prostate cancer [22]. GLOI has also been shown to serve as a pro-survival factor in invasive prostate cancer PC3 cells by eluding apoptosis through the NF-κB signaling pathway [23]. Overexpression and nuclear translocation of GLO1 occurs in murine fibrosarcoma, while GLO1 siRNA inhibits tumor cell proliferation and migration [24].…”