Abstract:Wound healing (WH) and the role fibroblasts play in the process, as well as healing impairment and fibroblast dysfunction, have been thoroughly reviewed by other authors. We treat these topics briefly, with the only aim of contextualizing the true focus of this review, namely, the microgravity-induced changes in fibroblast functions involved in WH. Microgravity is a condition typical of spaceflight. Studying its possible effects on fibroblasts and WH is useful not only for the safety of astronauts who will fac… Show more
“…Fibroblasts have a crucial role in normal wound healing as they organize the whole repair process by producing regulatory molecules and crosstalk with the other types of cells involved in the healing mechanisms. 30 Previous studies have also shown the safety and non-toxicity of chlorogenic acid and polyvinyl alcohol. 31,32 These results could support the use of CGA hydrogel as a wound dressing.Figure 4.Comparison of MTT cell viability on the NIH3T3 cell lines after exposure to different concentrations of CGA hydrogel after 24 h, 48 h and 72 h. The results are shown as the means ± SD ( n = 3).…”
Section: Resultsmentioning
confidence: 99%
“…Fibroblasts have a crucial role in normal wound healing as they organize the whole repair process by producing regulatory molecules and crosstalk with the other types of cells involved in the healing mechanisms. 30 Previous studies have also shown the safety and non-toxicity of chlorogenic acid and polyvinyl alcohol. 31,32 These results could support the use of CGA hydrogel as a wound dressing.…”
Chlorogenic acid (CGA) is a phenolic compound widely found in plants. Several studies have shown that CGA possesses antioxidant, antibacterial, anti-inflammatory and wound healing properties. Because of their three-dimensional structure, good permeability, excellent biocompatibility and moisturizing properties, hydrogels are ideal candidates for wound dressing. The aim of the present study was to preparation and characterization of Polyvinyl alcohol (PVA) hydrogel containing CGA microspheres and evaluation its wound healing activity. The double-emulsion solvent evaporation technique was applied for preparing the CGA containing microspheres. The microspheres were characterized using scanning electron microscopy (SEM) and Fourier transformation infrared spectroscopy (FTIR) and subsequently incorporated in the structure of a PVA hydrogel. The effects of prepared hydrogel on NIH3T3 cell line viability were evaluated using MTT method and wound healing activity was investigated in full thickness wound model in rabbit. SEM images showed formation of homogenous CGA microspheres with diameters in the range of 1–2 μm, embedded in the porous structure of the hydrogel. Infra-red results indicated successful incorporation of CGA microspheres into PVA hydrogel. The NIH3T3 cell viability percentage in CGA 2.5% hydrogel treated group significantly ( p < .05) increased after 24 h and 48 h comparing to control group. In vivo studies showed that CGA hydrogel significantly ( p < .001) stimulated the rate of wounds closures. Histological studies revealed that administration of CGA hydrogel significantly increased epithelialization and production of collagen fibers compared to the control group. It can be concluded that the CGA microsphere loaded PVA hydrogel has the potential for wound healing.
“…Fibroblasts have a crucial role in normal wound healing as they organize the whole repair process by producing regulatory molecules and crosstalk with the other types of cells involved in the healing mechanisms. 30 Previous studies have also shown the safety and non-toxicity of chlorogenic acid and polyvinyl alcohol. 31,32 These results could support the use of CGA hydrogel as a wound dressing.Figure 4.Comparison of MTT cell viability on the NIH3T3 cell lines after exposure to different concentrations of CGA hydrogel after 24 h, 48 h and 72 h. The results are shown as the means ± SD ( n = 3).…”
Section: Resultsmentioning
confidence: 99%
“…Fibroblasts have a crucial role in normal wound healing as they organize the whole repair process by producing regulatory molecules and crosstalk with the other types of cells involved in the healing mechanisms. 30 Previous studies have also shown the safety and non-toxicity of chlorogenic acid and polyvinyl alcohol. 31,32 These results could support the use of CGA hydrogel as a wound dressing.…”
Chlorogenic acid (CGA) is a phenolic compound widely found in plants. Several studies have shown that CGA possesses antioxidant, antibacterial, anti-inflammatory and wound healing properties. Because of their three-dimensional structure, good permeability, excellent biocompatibility and moisturizing properties, hydrogels are ideal candidates for wound dressing. The aim of the present study was to preparation and characterization of Polyvinyl alcohol (PVA) hydrogel containing CGA microspheres and evaluation its wound healing activity. The double-emulsion solvent evaporation technique was applied for preparing the CGA containing microspheres. The microspheres were characterized using scanning electron microscopy (SEM) and Fourier transformation infrared spectroscopy (FTIR) and subsequently incorporated in the structure of a PVA hydrogel. The effects of prepared hydrogel on NIH3T3 cell line viability were evaluated using MTT method and wound healing activity was investigated in full thickness wound model in rabbit. SEM images showed formation of homogenous CGA microspheres with diameters in the range of 1–2 μm, embedded in the porous structure of the hydrogel. Infra-red results indicated successful incorporation of CGA microspheres into PVA hydrogel. The NIH3T3 cell viability percentage in CGA 2.5% hydrogel treated group significantly ( p < .05) increased after 24 h and 48 h comparing to control group. In vivo studies showed that CGA hydrogel significantly ( p < .001) stimulated the rate of wounds closures. Histological studies revealed that administration of CGA hydrogel significantly increased epithelialization and production of collagen fibers compared to the control group. It can be concluded that the CGA microsphere loaded PVA hydrogel has the potential for wound healing.
“…The participation of fibroblasts in the healing process is critical since they are responsible for creating protein structural products that will be utilized throughout the tissue regeneration process. 11 Normal fibroblast division activity is limited, but when there is damage, these cells seem to be more active in creating extracellular matrix. Interleukin-Ib (IL-Ib), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF).…”
UV exposure causes inflammation and the generation of reactive oxygen species, both of which contribute to skin aging. The purpose of this research was to determine how a combination of Phaleria macrocarpa extract and soybean extract affected the number of fibroblasts, VEGF, IL-6, and TNF alpha expression, and blood levels of IL-6 and TNF alpha in UV-B-exposed mice. In this study, mice were placed into four groups: one control group, three treatment groups, and a combination of Phaleria macrocarpa:soybeans at a 1:1 ratio (com group). The mice were euthanized on days 5 and 21 for histological preparations and then examined under a light microscope. Using an Olympus C-21 microscope with an Optilab Advances camera at 1000x magnification, the fibroblast was studied by counting the number of fibroblast cells per field of view. The immunohistochemical approach was performed to analyze the expression of VEGF, IL-6, and TNF-in skin tissue. The ELISA technique was used to quantify the levels of IL-6 and TNF-alpha. SPSS ver 21 was used to analyze the data. On days 5 and 21, the number of fibroblasts and expression of VEGF, IL-6, and TNF alpha were significantly higher in the combination group than in the control, Phaleria macrocarpa, and soybean treatment groups. However, there was no significant change in IL-6 and TNF alpha levels across groups on days 5 and 21 (p > 0.05). Finally, a 1:1 mixture of Phaleria macrocarpa and soybeans reduced the number of fibroblasts and the production of VEGF, IL-6, and TNF alpha on days 5 and 21, but not in serum levels.
“…Fibroblasts are critical in supporting normal wound healing, involved in key processes such as breaking down the fibrin clot, creating new extra cellular matrix (ECM) and collagen structures to support the other cells associated with effective wound healing, as well as contracting the wound (Bainbridge, 2013). Healing problems such as fibrosis and delayed healing may occur if fibroblast dysfunction is present (Cialdai et al, 2022).…”
V esicovaginal fistula, one of the complications of gynecological and obstetric procedures, is an abnormal opening between the bladder and vagina. These cases are still common in developing countries, with an estimate reaching at least three million women. Medical records in Africa show that 30,000-130,000 cases of vesicovaginal research Article Abstract | Background: One of the complications of gynecological and obstetric procedures is a vesicovaginal fistula. Surgery is the gold standard but the success rate has not yet reached 100%. The wound healing process needs to be supported by certain ingredients such as regenerative medicine. Amnion is easy to find and rich in growth substances. Aim: This study analyzed the comparison of tissue structure in the remodeling phase of the vesicovaginal fistula between the use of freeze-dried amnion and primary suturing on day 21. Materials and Methods: This experimental study used New Zealand rabbits which were female and weighed 3-4.5 kg. The first stage of the study created a model of a vesicovaginal fistula. The second stage of the study closed the vesicovaginal fistula which was successfully created in the first stage. The study used a post-test-only control group design. The histological examination included fibroplasia, angiogenesis, re-epithelialization, and collagen deposition on day 21. Data were analyzed descriptively and statistically.
results:The mean scores of angiogenesis, fibroplasia, re-epithelialization, and collagen deposition in a New Zealand rabbit model of vesicovaginal fistula sutured with human amniotic stem cell seeding were higher than rabbits without amniotic stem cell seeding, the stem cell injection group, and the control group. There was a significant difference between groups only in angiogenesis at day 21. conclusion: Freeze-dried amnion plays a role in the wound healing process, especially in angiogenesis in the vesicovaginal fistula repair model. The use of freeze-dried amnion can be applied to assist the wound healing process in patients but still needs to be studied in humans.
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