Role of ethylene in auxin-induced flower bud formation in tobacco explants

Smulders, M.J.M.; Kemp, A.; Barendse, G. W. M.; Croes, A. F.; Wullems, G. J.

doi:10.1034/j.1399-3054.1990.780201.x

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In recent years there has been increasing evidence that the occurrence of morphogenesis in cultured plant cells may be associated with ethylene. The presence of ethylene was found to be important for embryogenesis from anther cultures of Hordeum vulgaris (6) and flower bud formation from thin-layer explants of Nicotiana tabacum (28). In contrast, shoot regeneration from callus cultures of Helianthus annuus (18) and N. tabacum (10) was inhibited by ethylene.…”

mentioning

confidence: 89%

Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro

Chi¹,

Pua²,

Goh³

1991

Plant Physiol.

View full text Add to dashboard Cite

The promotive effect of AgNO3 and aminoethoxyvinylglycine (AVG) on in vitro shoot regeneration from cotyledons of Brasska campestris ssp. peklnensls in relation to endogenous 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, ACC, and ethylene production was investigated. AgNO3 enhanced ACC synthase activity and ACC accumulation, which reached a maximum after 3 to 7 days of culture. ACC accumulation was concomitant with increased emanation of ethylene which peaked after 14 days. In contrast, AVG was inhibitory to endogenous ACC synthase activity and reduced ACC and ethylene production. The promotive effect of AVG on shoot regeneration was reversed by 2-chloroethylphosphonic acid at 50 micromolar or higher concentrations, whereas explants grown on AgNO3 medium were less affected by 2-chloroethylphosphonic acid. The distinctive effect of AgNO3 and AVG on endogenous ACC synthase, ACC, and ethylene production and its possible mechanisms are discussed.Plants usually exhibit enhanced ethylene production when wounded or under environmental stresses or pathogen attack (31). Ethylene biosynthesis in plants occurs via SAM' and ACC intermediates using methionine as a precursor (31), but the molecular mechanism of its action is not clear. It has been known for some time that cultured plant cells evolve ethylene (1 1 Cotyledonary explants excised from 3-d-old aseptically germinated seedlings of Brassica campestris ssp. pekinensis cv Shantung and cv Wong Bok were used. Because our preliminary investigations showed that explants cultured in 50-mL Erlenmyer flasks and 100-x 25-mm Petri dishes exhibited the similar capacity of shoot regeneration, both culture containers were used in this study. Explants were cultured either in a flask containing 25 mL medium or in a Petri dish containing 30 mL medium consisting of all constituents of Murashige and Skoog's medium (16)

show abstract

mentioning

confidence: 89%

Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro

Chi¹,

Pua²,

Goh³

1991

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Ethylene stimulated shoot morphogenesis in rice callus (Adkins et al 1990) and Pinus radiata cotyledons (Kumar et al1987), embryogenesis in anther culture of barley (Cho and Kasa 1989) and flower bud formation in tobacco explants (Smulders et al 1990). In contrast, ethylene appeared to inhibit to in vitro shoot regeneration in Nicotiana (Huxter et al 1981), Triticum (Purnhauser et al1987), Zea mays (Songstad et al 1988) and Brassica (Chi et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

2007

View full text Add to dashboard Cite

The interaction between plant hormones throughout plant regeneration and development is complex. In the present study, we extended our investigation and disclosed the capacity of de novo shoot organogenesis from cotyledon explants of bottle gourd in relation to plant hormones and ethylene inhibitor. Synergistic effect of kinetin and benzyl adenine (BA) enhanced the shoot regeneration efficiency (80.6%) from cotyledon explants of bottle gourd, compared with BA or kinetin used separately, even in the presence of AgNO 3 . The use of BA or kinetin separately or in synergistic combination led to a characteristic brownish color of the explants at the bud formation stage which was related to ethylene production. In the cotyledon explants cultured on MS medium supplemented with kinetin, the production of ethylene was lower than when BA or the combination of BA and kinetin was used. However, the regeneration capacity was very low and the emergence of shoot buds was delayed. BA promoted ethylene production while the synergistic effect of kinetin and BA decreased ethylene production and played a role in high frequency of regeneration from cotyledon explants of bottle gourd. The physiological relevance of the hormonal interaction in relation to the regulation of ethylene action and effect on regeneration was discussed.

show abstract

“…Explants were exposed to an elevated ethylene concentration by placing the culture dishes in a desiccator. Sterile ethylenecontaining air, 10 ML L-', was daily flushed through (21).…”

Section: Materials and Methods Plant And Tissue Culturementioning

confidence: 99%

“…Ethylene endogenously produced in untransformed explants has been found to reduce their sensitivity to NAA, especially at low auxin concentrations (21). To check whether a decrease in ethylene production might account for the increased responsiveness to NAA of the transformed tissues, we compared ethylene accumulation in T' and untransformed explants.…”

Section: Ethylene Production and Effectmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition by Ethylene of Auxin-Promotion of Flower Bud Formation in Tobacco Explants Is Absent in Plants Transformed by Agrobacterium rhizogenes

Smulders¹,

Croes²,

Kemp³

et al. 1991

Plant Physiol.

View full text Add to dashboard Cite

The in vitro regeneration of flower buds was studied in pedicel explants from tobacco (Nicotiana tabacum L., cv Petit Havana) transformed with Agrobacterium rhizogenes, pRi 1855 (agropine type). At a low concentration (0.1 micromolar) of 1-naphthaleneacetic acid, pedicel strips from phenotypically aberrant plants regenerated two to three times more flower buds than explants from untransformed tobacco. Intermediate bud numbers were observed in transformants with a less extreme phenotype. The results can be explained by an increased sensitivity of the transformed explants to auxin with respect to flower bud regeneration. The effect of transformation on the auxin response is fully accounted for by the absence of a negative interaction of endogenous ethylene with 1-naphthaleneacetic acid, a phenomenon normally encountered in untransformed tissues. Three observations led to this conclusion. Application of 1 micromolar AgNO3 to untransformed explants increased the number of flower buds to the level observed in transformed tissues but had no effect on transformed pedicel strips; exposure to 10 microliters per liter ethylene strongly reduced the response to auxin at all concentrations in untransformed explants but was almost ineffective in the transformed tissues; and endogenous ethylene synthesis occurred at the same rate in both types of explants.Agrobacterium rhizogenes causes the hairy root syndrome in dicotyledonous plants, which is characterized by neoplastic growth of numerous adventitious roots at the site of the bacterial infection (7). When a plant is infected, a piece of DNA (transferrable or T-DNA) is dissected from the bacterial Ri plasmid and transferred to the plant where it is incorporated in the nuclear genome (5,29). In some species, plants regenerate spontaneously from the transformed roots (1,5,23,25). The regenerants often display inheritable morphological abnormalities, for instance wrinkled leaves, shortened internodes, reduced apical dominance, and an overdeveloped, partially nongeotropic root system (1,6,26). In regenerants of tobacco, the flowers are smaller and show heterostyly (10,11,23,26).In the case of the agropine type of A. rhizogenes, the T-DNA is divided in a TL and a TR region. The gene products of genes located on the TL-DNA are generally considered to be the determinants of the altered phenotype (8,10,13,16,25,26), whereas the genes on the TR-DNA would only play a role in root induction (3, 4, 12). The mechanism that causes 1131 the aberrant phenotype as a result of transformation is not clear. Recently, a higher sensitivity of the tissue to exogenously supplied auxin with respect to a number of hormonemediated processes-such as cellular proton extrusion, rhizogenesis, and root elongation (14, 15, 24)-has been proposed. Theoretically, an increased responsiveness to the hormone could be caused by many factors, such as higher uptake of the exogenously applied auxin, lower conversion of auxin into biologically nonactive compounds, a change in the interaction between ethylene an...

show abstract

Role of ethylene in auxin-induced flower bud formation in tobacco explants

Cited by 7 publications

References 0 publications

Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro

Role of Ethylene on de Novo Shoot Regeneration from Cotyledonary Explants of Brassica campestris ssp. pekinensis (Lour) Olsson in Vitro

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

Inhibition by Ethylene of Auxin-Promotion of Flower Bud Formation in Tobacco Explants Is Absent in Plants Transformed by Agrobacterium rhizogenes

Contact Info

Product

Resources

About