Role of Endothelial Nitric Oxide Synthase in Glucocorticoid- Induced Hypertension: An Overview of Experimental Data

Blecharz-Lang, Kinga G; Burek, Malgorzata

doi:10.5772/67025

Cited by 2 publications

(2 citation statements)

References 110 publications

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“…however, when compared to the research that has been carried out by Ugusman et al, 2020 which used a dose of dexamethasone 0.02 mg/kg BW without the administration of a 5% NaCl solution instead of drinking water and caused hypertension after 28 days of administration, the addition of salt solution in this study did not accelerate the occurrence of hypertension. On the other hand, dexamethasone causes a decrease in nitric oxide (NO) levels only through a downregulation of eNOS mRNA (Blecharz-Lang & Burek, 2017). Purpose of the addition of a 5% NaCl solution in this study is to cause the uncoupling of eNOS so that it actually lowers the level of nitric oxide and causes endothelial dysfunction (Li et al, 2016) as a result of the research that has been done by Satoh et al, 2010(Satoh et al, 2010.…”

Section: Resultsmentioning

confidence: 94%

Dexamethasone and 5% NaCl Solution Induce Hypertension in Sparangue dawly Male Rats

Syafi’i

2022

fitofarmaka JIF

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High blood pressure can be caused by excess salt intake Dexamethasone is a potent anti-inflammatory drug, but the long-term administration can cause hypertension. This study aims to determine the accurate time and dose that cause hypertension in rat models by administration of a combination of dexamethasone and 5% NaCl solution. The control group was administrated with aquadest orally, 3 test groups were administrated with dexamethasone at a doses of 0.02mg/kg BW, 0.03mg/kg BW, and 0.5mg/kg BW intraperitonially (i.p) for 28 days. A 5% NaCl solution were administrated instead of drinking water to the test group. Blood pressure and body weight were measured weekly for 28 days. Results of the study show that Dexamethasone at a dose of 0.02mg/kg BW caused hypertension on day-28 where the rats blood pressure increase to 148mmHg/103mmHg (Â±1.9/Â±3.1) (P<0.05) compared to control rats and the weight decline by 90 grams. Dexamethasone 0.03mg/kg BW caused hypertension on day-21, with the increase of rats blood pressure at144mmHg/101mmHg (Â±2,6/Â±3.2) (P<0.05) compared to control and the weight decrease by 83 grams.Â Dexamethasone 0.5mg/kg BW caused hypertension on day-7, the rats blood pressure rise to 146mmHg/103mmHg (Â±1.6/1.9) (P<0.05) compared to control and the weight decrease by 69 grams. As conclusion, Dexamethasone at dose of 0.5mg/kg BW and NaCl 5% cause hypertension faster on day 14 compared to dexamethasone at dose of 0.02mg/kg BW and NaCl 5% which cause hypertension slower after 28 days of administration.

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Section: Resultsmentioning

confidence: 94%

Dexamethasone and 5% NaCl Solution Induce Hypertension in Sparangue dawly Male Rats

Syafi’i

2022

fitofarmaka JIF

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“…The attenuation of the insulin-mediated vasodilation in our study is due to reduction of NO bioavailability, mediated by a decrease in eNOS expression. Studies have demonstrated that GC treatment significantly down-regulates eNOS expression [16,34]. These changes in eNOS expression following GC treatment may have been caused by uncoupling of eNOS, through inhibiting essential cofactors, becoming inactive and reducing NO production [35].…”

Section: Plos Onementioning

confidence: 99%

Effects of high doses of glucocorticoids on insulin-mediated vasodilation in the mesenteric artery of rats

Araújo

Miguel-dos-Santos

Macedo³

et al. 2020

PLoS ONE

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Several pathological conditions predict the use of glucocorticoids for the management of the inflammatory response; however, chronic or high dose glucocorticoid treatment is associated with hyperglycemia, hyperlipidemia, and insulin resistance and can be considered a risk factor for cardiovascular disease. Therefore, we investigated the mechanisms involved in the vascular responsiveness and inflammatory profile of mesenteric arteries of rats treated with high doses of glucocorticoids. Wistar rats were divided into a control (CO) group and a dexamethasone (DEX) group, that received dexamethasone for 7 days (2mg/kg/day, i.p.). Blood samples were used to assess the lipid profile and insulin tolerance. Vascular reactivity to Phenylephrine (Phe) and insulin, and O 2 •production were evaluated. The intracellular insulin signaling pathway PI3K/AKT/eNOS and MAPK/ET-1 were investigated. Regarding the vascular inflammatory profile, TNF-α, IL-6, IL-1β and IL-18 were assessed. Dexamethasone-treated rats had decreased insulin tolerance test and endothelium-dependent vasodilation induced by insulin. eNOS inhibition caused vasoconstriction in the DEX group, which was abolished by the ET-A antagonist. Insulin-mediated relaxation in the DEX group was restored in the presence of the O 2 .scavenger TIRON. Nevertheless, in the DEX group there was an increase in Phe-induced vasoconstriction. In addition, the intracellular insulin signaling pathway PI3K/AKT/eNOS was impaired, decreasing NO bioavailability. Regarding superoxide anion generation, there was an increase in the DEX group, and all measured proinflammatory cytokines were also augmented in the DEX group. In addition, the DEX-group presented an increase in low-density lipoprotein cholesterol (LDL-c) and total cholesterol (TC) and reduced high-density lipoprotein cholesterol (HDL-c) levels. In summary, treatment with high doses of dexamethasone promoted changes in insulin-PLOS ONE PLOS ONE | https://doi.org/10.1371/journal.pone.0230514 March 18, 2020 1 / 16 OPEN ACCESS Citation: Araujo JEdS, Miguel-dos-Santos R, Macedo FN, Cunha PS, Fontes MT, Murata GM, et al. (2020) Effects of high doses of glucocorticoids on insulin-mediated vasodilation in the mesenteric artery of rats. PLoS ONE 15(3): e0230514. https://

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Role of Endothelial Nitric Oxide Synthase in Glucocorticoid- Induced Hypertension: An Overview of Experimental Data

Cited by 2 publications

References 110 publications

Dexamethasone and 5% NaCl Solution Induce Hypertension in Sparangue dawly Male Rats

Dexamethasone and 5% NaCl Solution Induce Hypertension in Sparangue dawly Male Rats

Effects of high doses of glucocorticoids on insulin-mediated vasodilation in the mesenteric artery of rats

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