Role of DNA and bacteriophage in campylobacter auto-agglutination

Ritchie, A. E.; Bryner, John H.; Foley, John W.

doi:10.1099/00222615-16-3-333

Cited by 12 publications

(8 citation statements)

References 8 publications

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“…Temperate bacteriophages were identified in C. fetus (originally characterized as Vibrio fetus) during the late 1960s and 1970s; however, their effect on Campylobacter typing methods was never addressed (2,20). In this study, we have identified the presence of bacteriophage genes in the genomes of three of four highly related C. jejuni strains isolated from a waterborne outbreak.…”

Section: Discussionmentioning

confidence: 87%

Temperate Bacteriophages Affect Pulsed-Field Gel Electrophoresis Patterns of Campylobacter jejuni

Barton

Tyler

et al. 2007

J Clin Microbiol

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The recently sequenced genome of Campylobacter jejuni RM1221 revealed the presence of three integrated bacteriophage-like elements. In this study, genes from the first element, a Mu-like bacteriophage, were amplified by PCR and used to probe pulsed-field gels of clinical C. jejuni strains obtained from a waterborne outbreak (Ontario, Canada, 2000). These highly similar strains differed only by their pulsed-field gel electrophoresis (PFGE) patterns due to an apparent insertion or deletion of a 40-kb fragment. Bacteriophage probes hybridized to these different bands in Southern blot analysis, indicating that homologues of bacteriophage genes were present in the outbreak strains. Investigation of the bacteriophage insertion sites in these isolates suggested that bacteriophage acquisition, loss, or transposition was responsible for the PFGE pattern variation. The bacteriophage gene sequences were similar, but not identical, in the outbreak strains and RM1221, indicating that differences may exist between the bacteriophages.

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Section: Discussionmentioning

confidence: 87%

Temperate Bacteriophages Affect Pulsed-Field Gel Electrophoresis Patterns of Campylobacter jejuni

Barton

Tyler

et al. 2007

J Clin Microbiol

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“…Consistent with this the Campylobacter bacteriophage reported here possessed 140 kb ds-DNA genomes with icosahedral heads and tail morphologies under the electron microscope that place them in the family Myoviridae. The most common C. jejuni bacteriophage found by Ritchie et al (1983) possessed contractile tails with head diameters of 60-70 nm, and overall lengths of 180-210 nm. The phage CP1-8 were distinguishable from these but of similar dimensions to those reported from retail poultry by Atterbury et al (2003a), and very similar to each other with head diameters in the range of 92-96 nm and overall lengths of 190-200 nm.…”

Section: Discussionmentioning

confidence: 99%

Free-range layer chickens as a source of Campylobacter bacteriophage

Loc-Carrillo

Connerton

Pearson

et al. 2007

Antonie van Leeuwenhoek

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Bacteriophage specific for Campylobacter were isolated from chicken excreta collected from established free-range layer breed stock. Bacteriophage were either propagated on a Campylobacter jejuni host with broad susceptibility to bacteriophage (NCTC 12662) or on Campylobacter isolates from the same samples. Campylobacters were confirmed as being C. jejuni and or C. coli, using a combination of standard biochemical tests and PCR analysis with genus and species specific primers. The bacteriophage displayed differential patterns of susceptibility against reference NCTC strains and contemporary C. jejuni /C. coli isolates from chicken excreta. Electron microscopy demonstrated that the phage possessed icosahedral heads and rigid contractile tails. Pulsed-field gel electrophoresis revealed the bacteriophage genomes to be double stranded DNA in the range of 140 kb in size and the restriction enzyme patterns of the DNAs indicate they are genetically related members of the Myoviridae family. This study showed that Campylobacter bacteriophage could easily be isolated from free-range chickens and form part of their normal microbiological biota of environmentally exposed birds.

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“…1982). Campylobacter bacteriophages were also reported to play a role in the auto‐agglutination of cells, which interfered with attempts to serotype Campylobacter isolates (Ritchie et al. 1983).…”

Section: The Bacteriophages Of Campylobactermentioning

confidence: 99%

Campylobacter bacteriophages and bacteriophage therapy

Connerton¹,

Timms²,

Connerton³

2011

Journal of Applied Microbiology

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Summary Members of the genus Campylobacter are frequently responsible for human enteric disease with occasionally very serious outcomes. Much of this disease burden is thought to arise from consumption of contaminated poultry products. More than 80% of poultry in the UK harbour Campylobacter as a part of their intestinal flora. To address this unacceptably high prevalence, various interventions have been suggested and evaluated. Among these is the novel approach of using Campylobacter‐specific bacteriophages, which are natural predators of the pathogen. To optimize their use as therapeutic agents, it is important to have a comprehensive understanding of the bacteriophages that infect Campylobacter, and how they can affect their host bacteria. This review will focus on many aspects of Campylobacter‐specific bacteriophages including: their first isolation in the 1960s, their use in bacteriophage typing schemes, their isolation from the different biological sources and genomic characterization. As well as their use as therapeutic agents to reduce Campylobacter in poultry their future potential, including their use in bio‐sanitization of food, will be explored. The evolutionary consequences of naturally occurring bacteriophage infection that have come to light through investigations of bacteriophages in the poultry ecosystem will also be discussed.

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Role of DNA and bacteriophage in campylobacter auto-agglutination

Cited by 12 publications

References 8 publications

Temperate Bacteriophages Affect Pulsed-Field Gel Electrophoresis Patterns of Campylobacter jejuni

Temperate Bacteriophages Affect Pulsed-Field Gel Electrophoresis Patterns of Campylobacter jejuni

Free-range layer chickens as a source of Campylobacter bacteriophage

Campylobacter bacteriophages and bacteriophage therapy

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