Role of Cyclooxygenase (COX)-2 in Healing of Gastric Ulcers in Rats

Shigeta, Jun-ichi; Takahashi, Satoru; Okabe, Susumu

doi:10.1016/s0021-5198(19)40604-5

Cited by 14 publications

(18 citation statements)

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“…These results were clearly demonstrated by the immunohistochemical stain of COX-2 in this study showing that I3C-treated ulcerated rats produced an increase in the COX-2 production in the stomach tissue verifying the healing process of I3C. These results were in agreement with Gilroy et al (1999) who explained that COX-2 produces prostaglandins that exert anti-inflammatory actions and play an important role in the healing of gastric ulcers (Shigeta et al, 1998). On the basis of the results made by Hatazawa et al (2007), they brought about that endogenous prostaglandin subtype 2 derived from COX-2, which plays an important part in the spontaneous healing of gastric ulcers and the upregulation of COX-2 appears to be a defensive and anti-inflammatory response aimed at enhancing mucosal defense.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of antiulcer activity of indole-3-carbinol and/or omeprazole on aspirin-induced gastric ulcer in rats

El-Shinnawy

Alshailabi

2012

Toxicol Ind Health

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The present work is an attempt to elucidate the antiulcer activity of indole-3-carbinol (I3C), which is one of the anticarcinogenic phytochemicals found in the vegetables of Cruciferae family such as broccoli and cauliflower, alone or in combination with omeprazole (OMP), a proton pump inhibitor, to diminish the effects of induced acute gastric ulcer by aspirin (ASA) in male albino rats. A total of 48 adult male albino rats were used in the present study. Animals were divided into eight experimental groups (six animals each group). They were given different experimental inductions of ASA at a dose of 500 mg/kg/body weight, OMP at a dose of 20 mg/kg/body weight and I3C at a dose of 20 mg/kg/body weight either alone or in combination with each other orally for a duration of 7 days. Inner stomach features, ulcer index, pH activity, body weight, stomach weight, hematological investigations, serum total protein albumin and reduced glutathione activity were investigated in addition to the histological, histochemical and immunohistochemical stain of cyclooxygenase-2 to the stomach tissue of normal control, ulcerated and treated ulcerated rats. The results of this study revealed that oral administration of ASA to rats produced the expected characteristic mucosal lesions. OMP accelerated ulcer healing but the administration of I3C either alone or in combination with OMP to ASA-ulcerated rats produced a profound protection to the gastric mucosa from injury induced by ASA. Our results suggested that administration of antiulcer natural substances such as I3C in combination with the perused treatment such as OMP is a very important initiative in the development of new strategies in ulcer healing.

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Section: Discussionsupporting

confidence: 91%

Evaluation of antiulcer activity of indole-3-carbinol and/or omeprazole on aspirin-induced gastric ulcer in rats

El-Shinnawy

Alshailabi

2012

Toxicol Ind Health

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“…Food consumption and body weight were recorded every day. We used acetate instead of acetic acid to prevent damage to the stomach because acute administration of acetic acid has been reported to induce gastric ulceration 18) . In the final week, blood glucose concentrations were measured using an auto-analyzer (Glutest Ace; Arkray, Inc., Kyoto, Japan) from the tail vein.…”

Section: Experiments 1: Long-term Experiments With Normal Fat Diet (Nfd)mentioning

confidence: 99%

Effects of acetate administration on endurance training-induced metabolic adaptations in mice fed high fat diet

Seike

Banjo

Nakano

et al. 2020

JPFSM

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We investigated the effects of chronic pre-exercise acetate administration on body weight and metabolic adaptations to endurance training in ICR mice fed with either a normal fat diet (NFD) or high fat diet (HFD), respectively. Mice were divided into a control group (Con), an acetate group (Ace), a training group (Tra), and an acetate+training group (Ace+Tra) with NFD or HFD, respectively. Mice received orally either water or acetate (72 mg/kg body weight/day) for 4 weeks. The mice in the training group were subjected to training using a treadmill (20-25 m/min×60 min, 5 times/week) immediately after administration. As a result, in the NFD mice, there was no effect of acetate in any of the measurements. In the HFD mice, the final body weight in the Ace, Tra and Ace+Tra groups was significantly lower than the Con group. Moreover, the acetate treatment tended to decrease blood glucose concentration at rest. Gastrocnemius muscle glycogen concentration in the Ace+Tra group was significantly higher than that of the Ace and Tra groups. Unexpectedly, a significant negative main effect of acetate treatment in the maximal activity of β-HAD was observed, though the endurance training increased enzyme activity of citrate synthase in the plantaris muscle. These findings show the possibility that acetate treatment with endurance training shifts the metabolic characteristics of mice toward a carbohydrate metabolism against a lipid metabolism with the HFD condition, but not with the NFD condition.

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“…PGE 2 has been known as an important mediator for bone formation,[ 7 8 ] gastric ulcer healing,[ 9 10 ] and dermal wound healing. [ 11 12 ] Additionally, PGE 2 has been used to treat gastric ulcer in spite of high price and low efficacy.…”

Section: Introductionmentioning

confidence: 99%

In-vitro wound healing effect of 15-hydroxyprostaglandin dehydrogenase inhibitor from plant

Karna¹

2017

Phcog Mag

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Background:Prostaglandins (PGs) have short existence in vivo because they are rapidly metabolized by NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) to 15-ketoprostaglandins. Inhibition of 15-PGDH causes elevated level of PGE2 in cellular system. It will be valuable for the therapeutic management of diseases requiring elevated PGE2 levels, like wound healing.Objective:Ninety-eight plant samples were screened for the discovery of potent 15-PGDH inhibitor. Among them, top five plant extracts as potent 15-PGDH inhibitor were chosen to determine PGE2 release from HaCaT (Keratinocyte cell line) cell line. Finally, top 15-PGDH inhibitor was selected to evaluate in vitro wound healing effect on HaCaT scratch model.Method:The inhibitory activity for 15-PGDH inhibitors was evaluated using fluorescence spectrophotometer by measuring the formation of NADH at 468 nm following excitation at 340 nm. Cell viability assay and PGE2 release was evaluated in HaCaT cell line after treatment of 15-PGDH inhibitors. Scratches were made using sterile 200 μL on HaCaT cell and wound-healing effect was evaluated after treatment of 15-PGDH inhibitor.Results:15-PGDH inhibitors elevated PGE2 levels in concentration-dependent manner. Ethanol extract of Artocarpus heterophyllus (EEAH), the most potent 15-PGDH inhibitor (IC50 = 0.62 µg/mL) with least cytotoxicity (IC50 = 670 µg/ml), elevated both intracellular and extracellular PGE2 levels. EEAH facilitated in-vitro wound healing in a HaCaT (Keratinocyte cell line) scratch model.Conclusion:EEAH might apply to treat dermal wounds by elevating PGE2 levels via COX-1 induction and 15-PGDH inhibition.SUMMARY Biological inactivation of 15-PGDH causes elevated level of PGE2 which will be useful for the management of disease that requires elevated level of PGE2. Abbreviations used: 15-PGDH: 15-hydroxyprostaglandin dehydrogenase, COX: Cyclooxygenase, DTT: Dithiothreitol, DMEM: Dulbecco's modified Eagle's media, EEAH: Ethanol extract of Artocarpus heterophyllus, MRP4: Multidrug resistance 4, PGs: Prostaglandins, PGT: Prostaglandin transporter, SDS: Sodium dodecylsulfate

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Role of Cyclooxygenase (COX)-2 in Healing of Gastric Ulcers in Rats

Cited by 14 publications

References 0 publications

Evaluation of antiulcer activity of indole-3-carbinol and/or omeprazole on aspirin-induced gastric ulcer in rats

Evaluation of antiulcer activity of indole-3-carbinol and/or omeprazole on aspirin-induced gastric ulcer in rats

Effects of acetate administration on endurance training-induced metabolic adaptations in mice fed high fat diet

In-vitro wound healing effect of 15-hydroxyprostaglandin dehydrogenase inhibitor from plant

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